FastQCFastQC Report
Thu 26 May 2016
SRR1364270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250409
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT156410.48120098116883137No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA146150.4496357227659658No Hit
TATCAACGCAGAGTACTTTTTTTTT117290.3608468964982561No Hit
GGTATCAACGCAGAGTACTTTTTTT85810.2639975461549608No Hit
GTACTTTTTTTTTTTTTTTTTTTTT77950.23981597392820408No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN74950.23058636620806797No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA49160.15124250517396426No Hit
GAGTACTTTTTTTTTTTTTTTTTTT44120.13573676420413552No Hit
ACGCAGAGTACTTTTTTTTTTTTTT41030.12623026825239533No Hit
AAACAAAAAAAAAAAAAAAAAAAAA37230.11453943180688954No Hit
GAACAAAAAAAAAAAAAAAAAAAAA32700.10060272414948396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51200.014.0278111
CGTTCGC551.9575506E-413.82120415
TATCACG2100.013.5727212
CTCAACG3950.013.4712994
TCGACCG1351.8189894E-1213.37246818
GTTCGCG500.001497516213.30311416
CGACCGT1352.7284841E-1112.668653519
GTTCGAC1405.2750693E-1112.21714616
CTTACGG701.0893787E-412.2169573
ACATGGT19300.012.0129893
CATGGTA14950.011.8219824
AACTCAT8400.011.5334276
GTATCAA136000.011.463251
ACCGTCC752.078666E-411.3979168
TATAACG2350.011.3202262
ACTCATA8100.011.1397677
GTATCAC3500.011.12894251
GGTCTCA4700.010.9152571
TACATGT22200.010.91322
TTGACCG1158.8080196E-710.74081518
Produced by FastQC (version 0.11.5)