##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364270_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3250409 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.044847894526505 28.0 18.0 31.0 2.0 33.0 2 24.69698797905125 30.0 23.0 31.0 2.0 34.0 3 24.388009632018615 30.0 22.0 31.0 2.0 34.0 4 27.299683824404866 33.0 23.0 35.0 2.0 37.0 5 27.806201927203624 33.0 26.0 35.0 2.0 37.0 6 28.32309287846545 35.0 27.0 35.0 2.0 37.0 7 28.257963843934718 35.0 27.0 35.0 2.0 37.0 8 26.50245830601626 32.0 17.0 35.0 2.0 37.0 9 26.309070643109838 32.0 17.0 37.0 2.0 39.0 10 27.979370903784723 34.0 24.0 37.0 2.0 39.0 11 28.35048943071472 34.0 25.0 38.0 2.0 39.0 12 27.077688377062703 33.0 17.0 38.0 2.0 39.0 13 26.765084332464006 33.0 17.0 37.0 2.0 39.0 14 27.24253747759128 33.0 17.0 38.0 2.0 40.0 15 26.813526236236733 32.0 17.0 38.0 2.0 40.0 16 27.58832288490464 33.0 17.0 38.0 2.0 40.0 17 28.1346627455191 34.0 20.0 39.0 2.0 40.0 18 28.6649243218315 34.0 25.0 39.0 2.0 41.0 19 29.30310462467954 36.0 27.0 40.0 2.0 41.0 20 29.306797698381956 36.0 27.0 40.0 2.0 41.0 21 29.47917292869913 36.0 27.0 40.0 2.0 41.0 22 29.37602529404761 36.0 27.0 40.0 2.0 41.0 23 29.051589200005292 36.0 26.0 40.0 2.0 41.0 24 29.013533989107216 36.0 26.0 40.0 2.0 41.0 25 28.74549941253547 36.0 25.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6297536236207293 1101 2 -0.8684775717034476 1101 3 -3.0381972594743836 1101 4 -3.0462359735741273 1101 5 -3.829588391729896 1101 6 -1.8866063417882835 1101 7 -1.4805090518150337 1101 8 -5.091620733420164 1101 9 -6.157069144646535 1101 10 -6.632964621077235 1101 11 -3.517866891000825 1101 12 -0.8763372436514594 1101 13 -0.432985959100602 1101 14 -7.3458027603626626 1101 15 -9.194770793289816 1101 16 -4.557701709853976 1101 17 -2.5970040786713646 1101 18 -6.75179709020469 1101 19 -3.5967086855935015 1101 20 -2.6266139265076163 1101 21 -2.511795327465343 1101 22 -2.544748297682066 1101 23 -7.00167298285351 1101 24 -3.9589743754967692 1101 25 -3.0205029279558495 1102 1 1.8045196490383688 1102 2 1.2978576023088948 1102 3 1.7520871936053446 1102 4 1.809501235411112 1102 5 1.4295868818154567 1102 6 0.8569257167044029 1102 7 0.5446971724069378 1102 8 1.9733024011880111 1102 9 -3.1925499368202424 1102 10 -0.49550169030721847 1102 11 0.1557585649080977 1102 12 -3.9767897439071085 1102 13 -5.489518665239608 1102 14 -5.7365554787728605 1102 15 -0.9574753131793727 1102 16 0.4351771632414305 1102 17 -3.991156514162384 1102 18 -1.452627100089817 1102 19 -0.7155654194108649 1102 20 -0.20785817438571996 1102 21 -0.04792035729116151 1102 22 0.002336015642406153 1102 23 0.19599378607310314 1102 24 0.2809419417817516 1102 25 0.3922216178395601 1103 1 -1.0459405907637347 1103 2 0.6765235213365415 1103 3 1.5489149501006452 1103 4 2.1131000470811436 1103 5 1.8638644579208616 1103 6 1.1438403932057604 1103 7 1.203512905554284 1103 8 2.656833437429416 1103 9 3.5568548848294625 1103 10 1.9669826105602226 1103 11 1.644790538593604 1103 12 2.7354848062548385 1103 13 2.9241864791001895 1103 14 -2.66286378807494 1103 15 1.4276289123322314 1103 16 1.861017581558972 1103 17 1.9353416806518418 1103 18 1.7088719086343538 1103 19 1.2418919420024679 1103 20 1.2763602565245833 1103 21 1.1982651770734272 1103 22 1.1824310436499168 1103 23 1.4976938498561907 1103 24 1.1964271522123227 1103 25 1.2942102657796504 1104 1 1.7221374508334328 1104 2 1.1413927081074569 1104 3 1.7409000328094493 1104 4 2.056947991973768 1104 5 1.2987191946306318 1104 6 0.7271663073010401 1104 7 0.6638469364981532 1104 8 1.9887175597397544 1104 9 -3.0776123425833504 1104 10 -0.4895860324054766 1104 11 0.14408577162600977 1104 12 1.6561838658674262 1104 13 1.715701603954848 1104 14 1.5447635552283927 1104 15 -3.3657962114639197 1104 16 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2.071937010435718 1106 4 2.17780567877319 1106 5 1.3987706544152587 1106 6 0.8744486532756426 1106 7 0.9471049594055003 1106 8 2.5152395035112853 1106 9 3.41558055732855 1106 10 1.6286128478889914 1106 11 0.8308329575241551 1106 12 1.9445602622641687 1106 13 -3.9839548684147843 1106 14 0.3175276434320935 1106 15 2.0851323622938587 1106 16 1.7404633245890793 1106 17 1.5629987997918207 1106 18 1.3031630874063396 1106 19 0.9654515900822815 1106 20 0.502025304647205 1106 21 0.1609210278968085 1106 22 0.2729869756406025 1106 23 0.5716396685941483 1106 24 0.4485878835824266 1106 25 0.5247759272694914 1107 1 1.7942888873487775 1107 2 1.2376620441984798 1107 3 2.0017989921517483 1107 4 1.8284164749959793 1107 5 1.5393679066406634 1107 6 0.6861501462058932 1107 7 0.361095968801024 1107 8 2.0372690313142527 1107 9 3.220078177471077 1107 10 1.6934705100056036 1107 11 0.6435444268833272 1107 12 2.074180638296813 1107 13 2.468795896564952 1107 14 -3.0373926221138134 1107 15 1.1034660929139655 1107 16 1.2544300675367133 1107 17 1.0333880673829192 1107 18 1.145130621302819 1107 19 0.8403332410706668 1107 20 0.8580965667342859 1107 21 0.4600348160753285 1107 22 0.2947970466170986 1107 23 0.8085341699257995 1107 24 0.96498324582927 1107 25 0.9809178711092628 1108 1 1.8686315202409318 1108 2 1.3371302527925018 1108 3 1.6328232255654385 1108 4 1.978548323620391 1108 5 1.3625924936830316 1108 6 0.7020134293245803 1108 7 0.47322516286531524 1108 8 -3.8634291391028768 1108 9 -5.012848275736438 1108 10 -1.752724522777747 1108 11 -0.1396004061045062 1108 12 1.0004650631577547 1108 13 2.135973913899324 1108 14 2.43010982842976 1108 15 -3.0945582296103993 1108 16 0.2185656001840499 1108 17 0.8298206504668997 1108 18 0.6794022353316542 1108 19 0.6731175862010019 1108 20 0.6520422584676311 1108 21 0.28892039327800134 1108 22 0.37318149352120855 1108 23 0.6576183818591268 1108 24 0.6403085584201413 1108 25 0.9856815988433176 1109 1 0.5025010141317274 1109 2 0.22030670804630503 1109 3 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1.6476495220892886 1110 15 2.2349715994324733 1110 16 1.8067747102177592 1110 17 1.2569433349939025 1110 18 0.8191983031988634 1110 19 0.1594957400487047 1110 20 0.11864660891922796 1110 21 -0.06009896202373355 1110 22 -0.3000409943945854 1110 23 0.06514571474873776 1110 24 0.28350140951583214 1110 25 0.49723545024711413 1111 1 1.0511241564285498 1111 2 0.9619009561481562 1111 3 1.0213272673658018 1111 4 1.7308009807722726 1111 5 -1.4405619873140978 1111 6 -0.25449307394593745 1111 7 -0.5408105667872185 1111 8 1.6908550033534198 1111 9 3.011591885706707 1111 10 1.5065412645036282 1111 11 1.3627425678915657 1111 12 2.4811844910347354 1111 13 2.7871416245833167 1111 14 2.8414057850273267 1111 15 3.242160016165851 1111 16 2.323777943467018 1111 17 1.7984491218149756 1111 18 1.201722826084488 1111 19 0.8209672900464327 1111 20 0.9447740268811664 1111 21 0.6808432580584949 1111 22 0.6258431655357448 1111 23 0.9046911376986806 1111 24 0.8018951195483659 1111 25 0.9037206218994314 1112 1 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-1.23522643307766 2204 4 1.6766775392933724 2204 5 1.9758642037856617 2204 6 1.7370328943388422 2204 7 1.7364238094793052 2204 8 3.2721581539823923 2204 9 -2.391363696558944 2204 10 0.4127774355422815 2204 11 1.444227710393907 2204 12 3.1371843776074897 2204 13 3.4150050271491814 2204 14 3.4781270795746053 2204 15 3.914809015825835 2204 16 3.5459317288739065 2204 17 2.6303268322646467 2204 18 2.5622387488351848 2204 19 2.0236969541332535 2204 20 1.9154757161713079 2204 21 1.632556611361089 2204 22 1.6154549153141744 2204 23 2.0315012452432235 2204 24 1.9550704269723127 2204 25 2.0136456930389137 2205 1 -2.774746934356422 2205 2 -3.376878513015882 2205 3 -0.12995145884586634 2205 4 -1.4778761152315987 2205 5 -2.389365657024655 2205 6 -0.8295688484095045 2205 7 -0.6638106224836022 2205 8 -4.186519802994791 2205 9 0.9000214493475731 2205 10 0.584065235653167 2205 11 0.38623169661178736 2205 12 2.0494784226264855 2205 13 -4.108367817690183 2205 14 -0.06626002758359206 2205 15 2.090260669282756 2205 16 1.754495127244116 2205 17 1.6740401479387153 2205 18 0.4247585301382095 2205 19 0.5448550686435567 2205 20 0.52771007046913 2205 21 0.38247302509133974 2205 22 0.5472557807801799 2205 23 0.7144875573961826 2205 24 0.29589528329840675 2205 25 0.7871338512733033 2206 1 -6.666474362807559 2206 2 -5.314229169058002 2206 3 -2.248040006123542 2206 4 -1.0709787554531616 2206 5 -0.8393498694630139 2206 6 -1.2088496040747039 2206 7 -1.7481394023522903 2206 8 -2.8086213499429746 2206 9 -5.477067903594122 2206 10 -2.4760445528593884 2206 11 -1.7545201729839448 2206 12 -7.2696874045879305 2206 13 -6.194372614984793 2206 14 -7.471322401017936 2206 15 -2.4852625302827853 2206 16 -6.038876345945383 2206 17 -2.8155554102005027 2206 18 -2.0917583773762125 2206 19 -2.1719959291760595 2206 20 -2.132135008385049 2206 21 -1.9524588100986158 2206 22 -2.0838134249099554 2206 23 -1.7535894079820196 2206 24 -1.6654766894590125 2206 25 -1.8790523778893942 2207 1 -6.722475290443118 2207 2 -4.751164668779033 2207 3 -0.8366836651550322 2207 4 0.10299671819453593 2207 5 0.5556844289745797 2207 6 0.02455174472951427 2207 7 0.1543444939198082 2207 8 1.5844899364854719 2207 9 -1.45594913156609 2207 10 -0.3394546238801901 2207 11 -0.17456010785591047 2207 12 -3.8573087002524105 2207 13 -4.238770552205342 2207 14 -0.7249651447408745 2207 15 -4.471799430120608 2207 16 -0.7809533682675074 2207 17 0.10710925403908789 2207 18 0.19470739451922014 2207 19 -0.030714338214263393 2207 20 0.06001832613183211 2207 21 -0.20917106324035828 2207 22 -0.17095123143895563 2207 23 -0.21837157203411195 2207 24 -0.302480869003265 2207 25 0.1544445166858388 2208 1 -3.7632054682829725 2208 2 -4.201316990611101 2208 3 -1.3723111147498948 2208 4 -0.29575397182283325 2208 5 -2.8362251140652397 2208 6 -1.158542630519861 2208 7 -0.6657692931983448 2208 8 -4.780301538804867 2208 9 -7.850990383846003 2208 10 -3.5842697842325215 2208 11 -1.4166582600704238 2208 12 0.15909179725077394 2208 13 0.9364923185113057 2208 14 0.9699132660966612 2208 15 1.264809238782096 2208 16 -4.442064148796028 2208 17 -6.326293696445955 2208 18 -2.5937175276064437 2208 19 -2.1493151875399725 2208 20 -1.2832226914226368 2208 21 -1.2213025647447147 2208 22 -0.948124288060491 2208 23 -0.6871195630807563 2208 24 -0.7639534338064564 2208 25 -0.5199752975845975 2209 1 0.8306053669821623 2209 2 0.7217251524017172 2209 3 -2.055423529139677 2209 4 0.22196458602887148 2209 5 0.5311111120183867 2209 6 0.3155829729620052 2209 7 0.18931963575755617 2209 8 1.6551011016553296 2209 9 -3.4021375112392924 2209 10 -0.5699673135067584 2209 11 0.0678043686338512 2209 12 -3.9504622040569046 2209 13 0.3334006421030722 2209 14 1.3706954974770582 2209 15 2.2296894009874535 2209 16 -2.7455881526794457 2209 17 -0.1047366306722104 2209 18 0.4449442781830193 2209 19 0.3097000885591754 2209 20 0.49649926111776566 2209 21 0.38689831628927607 2209 22 0.19841293133282178 2209 23 0.6772136183501658 2209 24 0.30097811104108274 2209 25 0.4633174183644968 2210 1 -0.17926105614946763 2210 2 -2.7589695522858086 2210 3 -0.7830343139628262 2210 4 -2.7705355845506254 2210 5 -3.210009149151947 2210 6 -1.3917824923001376 2210 7 -0.7577535874601509 2210 8 -5.028615799361322 2210 9 -4.351644090359311 2210 10 -1.7045205264372107 2210 11 -0.7559820763319571 2210 12 0.8057684089270793 2210 13 -6.818298959528192 2210 14 -1.6315792982211867 2210 15 -4.873232494596898 2210 16 -1.2919486685118464 2210 17 -0.6420656181647963 2210 18 -0.24951398757458776 2210 19 -0.5558258998834091 2210 20 -0.5066536253497667 2210 21 -0.6452487284865285 2210 22 -0.49913654984307243 2210 23 -0.12538979219007373 2210 24 -0.2774940740035561 2210 25 -0.19642004666670587 2211 1 0.48166780284924116 2211 2 0.40652347902576125 2211 3 -3.2238148781017095 2211 4 -2.2958908090299346 2211 5 -0.42677348818683924 2211 6 -0.531093165644041 2211 7 -0.7324334623559494 2211 8 -4.479432799994367 2211 9 0.4518856950121872 2211 10 0.23987245285206882 2211 11 -5.616694802571455 2211 12 -0.28398497617783036 2211 13 0.9771183783095587 2211 14 1.4098722280793154 2211 15 -3.766592862806057 2211 16 -0.6635264272078203 2211 17 -5.618830876469037 2211 18 -1.8192596842449547 2211 19 -1.0068750160268216 2211 20 -0.4921951899044146 2211 21 -0.4227875310546345 2211 22 -0.3275772809964863 2211 23 -0.10851848363104821 2211 24 -0.10305456714977979 2211 25 0.21574460283051522 2212 1 1.970293317390066 2212 2 1.251683592433622 2212 3 2.193307982271822 2212 4 2.548113502251251 2212 5 -0.8652909421914856 2212 6 0.7065520058881347 2212 7 1.2407689172038978 2212 8 -1.0978384614752414 2212 9 2.888719855509933 2212 10 2.165859897777402 2212 11 1.826322203037666 2212 12 3.367484145802461 2212 13 3.465932441775628 2212 14 3.6654278764703854 2212 15 4.117631412937694 2212 16 3.319655120063093 2212 17 2.702405246942778 2212 18 2.2983887179630464 2212 19 1.8744597544002382 2212 20 1.747588076308844 2212 21 1.4637308030306677 2212 22 1.5646242574736249 2212 23 1.7087127157852002 2212 24 1.76559924365516 2212 25 2.0699152382153265 2213 1 2.023389523174572 2213 2 1.6677413776937158 2213 3 2.169576427272066 2213 4 2.2888028858079643 2213 5 1.2654580640660775 2213 6 -0.4454089213661163 2213 7 0.6626885371068738 2213 8 -5.789971350741936 2213 9 0.5330194151959375 2213 10 0.7920806719312772 2213 11 0.8083980755359619 2213 12 2.488253058283366 2213 13 -1.9385925790515302 2213 14 1.1348971262829686 2213 15 -3.313728510995656 2213 16 0.017959233855968648 2213 17 0.9969884878442556 2213 18 1.2275991167408407 2213 19 1.1137502394617123 2213 20 1.3263716521427718 2213 21 1.130687321239158 2213 22 1.1260004260368497 2213 23 1.3701571332024258 2213 24 1.339751014489611 2213 25 1.6473119855244072 2214 1 1.8804845253270948 2214 2 1.1594525626783003 2214 3 0.8036012645822055 2214 4 1.4995017564377946 2214 5 1.3163954631249268 2214 6 0.1291238957161447 2214 7 0.4013989595284606 2214 8 1.8534829532157815 2214 9 2.6326810245391172 2214 10 0.9542123148900217 2214 11 0.9957972674213984 2214 12 2.2517044726967086 2214 13 0.4959540525007 2214 14 2.0215403803675756 2214 15 2.987147691451252 2214 16 1.9214928858728761 2214 17 1.0314682554461285 2214 18 0.5872489444058608 2214 19 0.5502326794930248 2214 20 0.13086005112765164 2214 21 0.20749465674399659 2214 22 0.14707600704546664 2214 23 -5.451175899291911 2214 24 -2.2710687327524184 2214 25 -1.0528943268795068 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 377657.0 3 3.0 4 2502.0 5 17663.0 6 28032.0 7 26334.0 8 21118.0 9 16830.0 10 14957.0 11 13822.0 12 12581.0 13 12199.0 14 11685.0 15 10978.0 16 10314.0 17 10827.0 18 13519.0 19 19474.0 20 28472.0 21 38043.0 22 47714.0 23 56247.0 24 65312.0 25 74344.0 26 82986.0 27 90040.0 28 96488.0 29 109503.0 30 118551.0 31 128396.0 32 159743.0 33 196610.0 34 233739.0 35 265867.0 36 294333.0 37 285715.0 38 257811.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.65017432865578 20.221661415010768 15.959954992856595 20.168209263476854 2 13.958644253092334 23.92423048709237 41.09816807466146 21.018957185153837 3 14.794369596710702 32.32564481617402 31.702700935109934 21.17728465200534 4 8.77774745662463 19.4131560327913 40.04150773786224 31.767588772721826 5 9.543411637917071 42.733328664204365 33.96075525115029 13.762504446728272 6 25.657085206042503 40.78790660151393 19.822071668218534 13.732936524225037 7 24.697444317224395 37.85718915015292 21.711606892103337 15.733759640519345 8 23.447362776580167 36.072735240699394 22.891571470675828 17.588330512044614 9 24.94855511400903 18.632231181758556 23.44547243335005 32.973741270882364 10 14.391171813057785 34.686329439192484 30.490002521474974 20.432496226274758 11 30.15413550542323 28.414517168415156 23.42315260840903 18.008194717752588 12 20.84508189840351 32.15657359992494 29.95587806841245 17.042466433259097 13 25.40385376268688 26.997603203300578 27.68713747909824 19.911405554914296 14 20.35960206202875 26.158008631288915 29.875561058468588 23.60682824821375 15 20.731998236000102 32.36581827406374 27.411805405752382 19.490378084183778 16 20.177692271845906 32.85730024895682 26.71454463925025 20.25046283994703 17 18.973434580780392 32.26484687897523 27.862551838088024 20.899166702156354 18 18.778409082141884 30.55060500601627 30.73397850437958 19.93700740746227 19 20.66382996581826 30.526544465733245 30.555119253451267 18.254506314997226 20 22.06128797618033 28.984125984407555 30.089218784539938 18.865367254872176 21 23.254558718966642 28.652962118069958 29.08118297090715 19.01129619205625 22 22.299839642101908 29.152454809070406 29.13794976440104 19.409755784426643 23 21.063163999122256 29.520903694645973 29.748549514045923 19.667382792185848 24 20.53551394802265 29.64249174013484 30.080847143498662 19.741147168343844 25 20.386587963560206 28.9982636842115 30.59631614650234 20.018832205725953 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 24928.0 1 24928.0 2 24423.0 3 23918.0 4 23918.0 5 23918.0 6 29905.5 7 35893.0 8 35893.0 9 35893.0 10 36546.0 11 37199.0 12 37199.0 13 37199.0 14 43148.0 15 49097.0 16 49097.0 17 49097.0 18 68820.5 19 88544.0 20 88544.0 21 88544.0 22 120720.5 23 152897.0 24 152897.0 25 152897.0 26 196331.5 27 239766.0 28 239766.0 29 239766.0 30 269018.0 31 298270.0 32 298270.0 33 298270.0 34 314930.0 35 331590.0 36 331590.0 37 331590.0 38 344398.5 39 357207.0 40 357207.0 41 357207.0 42 367668.0 43 378129.0 44 378129.0 45 378129.0 46 373451.5 47 368774.0 48 368774.0 49 368774.0 50 337684.0 51 306594.0 52 306594.0 53 306594.0 54 265596.5 55 224599.0 56 224599.0 57 224599.0 58 188516.0 59 152433.0 60 152433.0 61 152433.0 62 121682.0 63 90931.0 64 90931.0 65 90931.0 66 69095.0 67 47259.0 68 47259.0 69 47259.0 70 35262.0 71 23265.0 72 23265.0 73 23265.0 74 16732.5 75 10200.0 76 10200.0 77 10200.0 78 7586.0 79 4972.0 80 4972.0 81 4972.0 82 3626.0 83 2280.0 84 2280.0 85 2280.0 86 1660.5 87 1041.0 88 1041.0 89 1041.0 90 777.5 91 514.0 92 514.0 93 514.0 94 299.5 95 85.0 96 85.0 97 85.0 98 54.5 99 24.0 100 24.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2540295698172138 2 0.2671356127798071 3 0.272734908130023 4 0.3388188994061978 5 0.2849487556796699 6 0.3146680925385082 7 0.31316058994421936 8 0.3126991095582125 9 0.3240822924130471 10 0.3150988075654479 11 0.31605253369652864 12 0.31679090231413953 13 0.315375695797052 14 0.3108839533732524 15 0.3090687971882923 16 0.3103609422691114 17 0.30860731680228554 18 0.30851502072508413 19 0.3011928652671095 20 0.35087892016050903 21 0.3084227246478828 22 0.31248375204474266 23 0.3165140140825355 24 0.31823687419029417 25 0.31426814287063565 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3250409.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.65296112270802 #Duplication Level Percentage of deduplicated Percentage of total 1 92.74032690944544 68.30599692368618 2 4.055360092957287 5.9737855853032915 3 1.2677794091905634 2.801271226118469 4 0.5989439847226288 1.7645599208582239 5 0.3229723199354163 1.1893933861957018 6 0.19519121596412875 0.8625846624540048 7 0.13845827662312557 0.7138503445668154 8 0.09953075114170935 0.5864587635483396 9 0.07144937613217361 0.4736212310254253 >10 0.433345441523662 5.893360271157477 >50 0.041165277805449735 2.1048061447930215 >100 0.03182715309074605 4.439606923288341 >500 0.0021734713254675077 1.1244141341542195 >1k 0.0012302667852218013 1.7771584793972375 >5k 1.2302667852218013E-4 0.7210059835767948 >10k+ 1.2302667852218013E-4 1.2681260198764917 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 15641 0.48120098116883137 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 14615 0.4496357227659658 No Hit TATCAACGCAGAGTACTTTTTTTTT 11729 0.3608468964982561 No Hit GGTATCAACGCAGAGTACTTTTTTT 8581 0.2639975461549608 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 7795 0.23981597392820408 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 7495 0.23058636620806797 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 4916 0.15124250517396426 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 4412 0.13573676420413552 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4103 0.12623026825239533 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 3723 0.11453943180688954 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 3270 0.10060272414948396 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.076535906712048E-5 0.0 5 0.0 0.0 0.0 3.076535906712048E-5 0.0 6 0.0 0.0 0.0 3.076535906712048E-5 0.0 7 0.0 0.0 0.0 3.076535906712048E-5 0.0 8 0.0 0.0 0.0 3.076535906712048E-5 0.0 9 0.0 0.0 0.0 3.076535906712048E-5 0.0 10 0.0 0.0 0.0 3.076535906712048E-5 0.0 11 0.0 0.0 0.0 6.153071813424096E-5 0.0 12 0.0 0.0 0.0 6.153071813424096E-5 0.0 13 0.0 0.0 0.0 6.153071813424096E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5120 0.0 14.027811 1 CGTTCGC 55 1.9575506E-4 13.821204 15 TATCACG 210 0.0 13.572721 2 CTCAACG 395 0.0 13.471299 4 TCGACCG 135 1.8189894E-12 13.372468 18 GTTCGCG 50 0.0014975162 13.303114 16 CGACCGT 135 2.7284841E-11 12.6686535 19 GTTCGAC 140 5.2750693E-11 12.217146 16 CTTACGG 70 1.0893787E-4 12.216957 3 ACATGGT 1930 0.0 12.012989 3 CATGGTA 1495 0.0 11.821982 4 AACTCAT 840 0.0 11.533427 6 GTATCAA 13600 0.0 11.46325 1 ACCGTCC 75 2.078666E-4 11.397916 8 TATAACG 235 0.0 11.320226 2 ACTCATA 810 0.0 11.139767 7 GTATCAC 350 0.0 11.1289425 1 GGTCTCA 470 0.0 10.915257 1 TACATGT 2220 0.0 10.9132 2 TTGACCG 115 8.8080196E-7 10.740815 18 >>END_MODULE