FastQCFastQC Report
Thu 26 May 2016
SRR1364270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364270_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250409
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT582401.7917745120690967No Hit
TATCAACGCAGAGTACTTTTTTTTT499281.5360528475031912No Hit
GGTATCAACGCAGAGTACTTTTTTT399321.228522318268255No Hit
GTACTTTTTTTTTTTTTTTTTTTTT282770.8699520583409658No Hit
ACGCAGAGTACTTTTTTTTTTTTTT193050.5939252567907608No Hit
GAGTACTTTTTTTTTTTTTTTTTTT173580.5340251026870771No Hit
GCAGAGTACTTTTTTTTTTTTTTTT76820.2363394883536195No Hit
ATCAACGCAGAGTACTTTTTTTTTT73740.22686375776094636No Hit
GTGGTATCAACGCAGAGTACTTTTT64960.19985177250001462No Hit
GTGGTATCAACGCAGAGTACATGGG59100.181823272086682No Hit
GGTATCAACGCAGAGTACATGGGAT49950.15367296854026677No Hit
GGTATCAACGCAGAGTACATGGGAG35730.10992462794682145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3250.015.7528752
TATAACG3900.015.5583942
GCGTATC551.1524855E-515.5118071
ATCACGC3500.014.6247433
TCACGCA3400.014.4975194
GGTATCA99550.014.2072541
GGACCGA1257.2759576E-1213.6501816
ATAACGC4600.013.6003053
CTATTCG500.00152998813.2652949
TAACGCA4900.012.9612824
GTGGTAT25000.012.6645291
AGGACCG1750.012.4584985
TAAACGC3700.012.2972714
TGGTATC25000.012.2113952
CGACCGT1302.417437E-911.75237419
GTATCAC5350.011.694261
TCGCAAT907.687808E-611.580811510
ACCGAGT1251.8948413E-811.3749768
CGGTTTG1950.011.26129414
CGTTCAA2050.011.17770214
Produced by FastQC (version 0.11.5)