FastQCFastQC Report
Thu 26 May 2016
SRR1364269_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364269_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575761
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA154290.9791459491636104No Hit
GTATCAACGCAGAGTACTTTTTTTT97940.6215409570359972No Hit
TATCAACGCAGAGTACTTTTTTTTT67560.42874522214980576No Hit
GGTATCAACGCAGAGTACTTTTTTT62430.39618952366507354No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA49800.31603777476406636No Hit
GAACAAAAAAAAAAAAAAAAAAAAA49370.3133089345401999No Hit
AAACAAAAAAAAAAAAAAAAAAAAA48420.30728010148747176No Hit
GTACTTTTTTTTTTTTTTTTTTTTT40260.2554955986345645No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN37210.23613987146527934No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29440.18683036323401836No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27200.1726150095096909No Hit
GAAAACAAAAAAAAAAAAAAAAAAA19490.12368626968176011No Hit
GTATCAACTCAGAGTACTTTTTTTT17640.11194591057907893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG402.7558836E-416.6291542
CCGAGTA456.7708094E-414.77535210
CGCGCCA551.9629048E-413.81591310
ACATGGT8550.013.2259473
GGTCTCA2500.012.9228161
GAACAAA14750.012.4332171
TAACGCA1001.4371653E-712.3534794
TACATGA8250.012.3242892
ACTCATA2550.012.2917077
GTTCGAC2750.012.09238816
TACGCTG550.003072979212.0885385
GGTATCA34400.012.0433221
CCTCTAG2850.012.0026151
TACATGT11850.011.7877542
CGACCGT2750.011.74614319
TTGCGCG5500.011.74614318
TCGACCG2750.011.74614318
CATGGTA7950.011.713994
CTAAGTG658.004568E-411.6956013
GACGTCT658.0174115E-411.69336615
Produced by FastQC (version 0.11.5)