FastQCFastQC Report
Thu 26 May 2016
SRR1364269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575761
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT427642.713863333335449No Hit
TATCAACGCAGAGTACTTTTTTTTT356652.2633508507952667No Hit
GGTATCAACGCAGAGTACTTTTTTT316182.0065225627490464No Hit
GTACTTTTTTTTTTTTTTTTTTTTT167311.061772692686264No Hit
ACGCAGAGTACTTTTTTTTTTTTTT166211.0547919386252103No Hit
GAGTACTTTTTTTTTTTTTTTTTTT129690.8230309037982282No Hit
GCAGAGTACTTTTTTTTTTTTTTTT58460.3709953476447253No Hit
ATCAACGCAGAGTACTTTTTTTTTT54790.347705013641028No Hit
GTGGTATCAACGCAGAGTACTTTTT52040.33025312848839383No Hit
GTGGTATCAACGCAGAGTACATGGG32570.20669378160774382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA30780.19533419090839282No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT28160.17870730396297407No Hit
CAACGCAGAGTACTTTTTTTTTTTT23520.14926121410543858No Hit
GGTATCAACGCAGAGTACATGGGAT22200.14088430923217418No Hit
GGTCGGGAGTGGGTAATTTGCGCGC20510.1301593325383735No Hit
AACGCAGAGTACTTTTTTTTTTTTT19100.12121127506011381No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT17110.10858245634966215No Hit
TCAACGCAGAGTACTTTTTTTTTTT16860.10699592133578634No Hit
GGTATCAACGCAGAGTACATGGGAG16370.10388631270858968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC250.00611738618.9538751
AGGCGTA350.002211823416.23947713
CAAGACT1650.014.9343034
TATCACG1300.014.5826822
CGACCGT2200.014.32912519
TATAACG1400.014.2181142
TCGACCG2400.013.93109318
ATAACGC1650.013.7855113
CGTTTAA852.7801434E-713.3792061
GTTCGAC2550.013.11161816
TTTAGAC802.0532934E-613.0316163
GGTATCA77450.013.0193181
TAGTCAA2650.012.8694289
GTATAAA2800.012.8615581
CCTCTAG3050.012.7394891
TTCGACC2700.012.736999517
AGATAGT3000.012.6367196
ATCACGC1501.8189894E-1212.6367193
AAACGCA2100.012.6367195
TGCACCG751.5150328E-512.6367195
Produced by FastQC (version 0.11.5)