##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364269_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1575761 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.58283204115345 31.0 28.0 33.0 23.0 34.0 2 28.54474377776833 31.0 28.0 33.0 22.0 34.0 3 28.57626442081001 31.0 28.0 34.0 22.0 34.0 4 32.006191928852154 35.0 32.0 37.0 24.0 37.0 5 31.383640666319323 35.0 32.0 37.0 17.0 37.0 6 31.332703373163824 35.0 32.0 37.0 17.0 37.0 7 30.914608243255163 35.0 31.0 37.0 11.0 37.0 8 30.691213959477356 35.0 31.0 37.0 10.0 37.0 9 30.943207758029295 37.0 30.0 39.0 8.0 39.0 10 30.99868888746453 37.0 30.0 39.0 8.0 39.0 11 31.50941925837738 37.0 30.0 39.0 8.0 39.0 12 31.354595652513293 37.0 30.0 39.0 8.0 39.0 13 31.387996022239413 37.0 30.0 39.0 7.0 39.0 14 31.721874700541516 37.0 30.0 40.0 7.0 41.0 15 31.99607745083169 37.0 30.0 40.0 2.0 41.0 16 32.03715347695494 37.0 31.0 40.0 2.0 41.0 17 32.19761943594238 38.0 31.0 40.0 2.0 41.0 18 32.14590156756005 38.0 31.0 40.0 2.0 41.0 19 32.07581987369912 38.0 31.0 40.0 2.0 41.0 20 31.807063380804575 38.0 30.0 40.0 2.0 41.0 21 31.3871253318238 37.0 30.0 40.0 2.0 41.0 22 31.40736571091682 37.0 30.0 40.0 2.0 41.0 23 31.294512302309805 37.0 30.0 40.0 2.0 41.0 24 31.27404663524481 37.0 30.0 40.0 2.0 41.0 25 31.143631553262203 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.47519198253685957 1101 2 0.41276386692106826 1101 3 0.5374516343670628 1101 4 0.5391756056797554 1101 5 0.8085894631294188 1101 6 0.5686168737886774 1101 7 0.7707722992289838 1101 8 0.8314567168423679 1101 9 1.6619795593267987 1101 10 1.701562314804086 1101 11 0.821822847001183 1101 12 0.8912938479217232 1101 13 0.7366718771742562 1101 14 1.3537867365107452 1101 15 1.0527467616942872 1101 16 0.8459973063258097 1101 17 0.6897493623967819 1101 18 0.9225935724360568 1101 19 0.7881903326586226 1101 20 1.481885194980137 1101 21 1.9220152659378265 1101 22 1.8127404499023463 1101 23 1.6561287660969448 1101 24 1.4544120014432735 1101 25 1.6785933310045706 1102 1 0.3640348035493375 1102 2 0.6843503315089023 1102 3 0.47801025343639836 1102 4 0.4521098320598078 1102 5 0.8521839133742226 1102 6 0.4823966551002812 1102 7 0.8295276876000273 1102 8 0.6892652847135423 1102 9 1.7289618328267586 1102 10 1.8146920364996717 1102 11 1.1238455199347577 1102 12 1.1247386236360768 1102 13 0.7277866402115727 1102 14 1.3915766343280076 1102 15 0.7991468712520771 1102 16 0.74750083617214 1102 17 0.8841591512342006 1102 18 0.7632724286628232 1102 19 0.881671267467965 1102 20 1.3338527749215174 1102 21 2.0368637382223476 1102 22 1.7676106829764855 1102 23 1.7444597311226353 1102 24 1.7367381444186947 1102 25 1.729204756424238 1103 1 0.47375014650614844 1103 2 0.6000620383590451 1103 3 0.5856955439073595 1103 4 0.5578485848623735 1103 5 0.7856238887052349 1103 6 0.46009434430952467 1103 7 0.7660885474171941 1103 8 0.7486353260212582 1103 9 1.4813930776472368 1103 10 1.551954420783467 1103 11 0.7901113154776347 1103 12 0.9269511238769894 1103 13 0.7551473792534615 1103 14 1.4675031669855123 1103 15 1.027795023326135 1103 16 0.8460226239031705 1103 17 0.9276472439484991 1103 18 0.4907276036906083 1103 19 0.6588010559185093 1103 20 1.3708536935845075 1103 21 2.072089003381894 1103 22 1.6831283784814595 1103 23 1.5616663532052364 1103 24 1.5478212031167615 1103 25 1.4180336859570062 1104 1 0.14005455630739405 1104 2 0.40130893211233953 1104 3 0.2632537132767965 1104 4 0.3601965034989014 1104 5 0.5145312263370272 1104 6 0.4096260909823357 1104 7 0.5287481360384696 1104 8 0.5669428194999213 1104 9 1.222044759890668 1104 10 1.4082118151948357 1104 11 0.7289839439206425 1104 12 0.5206405134609042 1104 13 0.5870958690117583 1104 14 1.1957973327058546 1104 15 0.26843922206890625 1104 16 0.15637705674884828 1104 17 0.060919125466867285 1104 18 0.5189079726865842 1104 19 0.6992130285692895 1104 20 1.351968901483282 1104 21 1.7572962607145364 1104 22 1.8305666455402765 1104 23 1.5867492656411493 1104 24 1.3701593338095677 1104 25 1.3963836809680004 1105 1 -0.3260831081361282 1105 2 0.13629956981056424 1105 3 0.08835988370929826 1105 4 0.41521257384163945 1105 5 0.6557077395069868 1105 6 0.44537174523349776 1105 7 0.16815642891443616 1105 8 0.47167367745353417 1105 9 1.397770579574665 1105 10 1.502402362165597 1105 11 0.7799278955841409 1105 12 1.0960422720293437 1105 13 0.5892166234009402 1105 14 0.8479407689601608 1105 15 0.9302226922977539 1105 16 0.3768609526246145 1105 17 0.4752019441953266 1105 18 0.4912995581972126 1105 19 0.5046255489588347 1105 20 1.0384847739629421 1105 21 1.8389174349595159 1105 22 1.3027577137113475 1105 23 1.4779494258619899 1105 24 1.3066554199813538 1105 25 1.0481584162658102 1106 1 0.2370498098058107 1106 2 0.2928960387402135 1106 3 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0.5128400266483908 1107 15 0.22694036133779605 1107 16 0.19768608013054134 1107 17 0.08912912694541575 1107 18 0.15138641372415407 1107 19 0.36449526566842394 1107 20 0.6423882229353239 1107 21 1.3711549747262204 1107 22 1.0547548858569833 1107 23 1.0931693420363047 1107 24 0.6833811686053011 1107 25 0.7749826348185564 1108 1 -0.2784774222183337 1108 2 -0.09197214552352762 1108 3 0.04208255646067727 1108 4 -0.9641799826629374 1108 5 -0.10878980913474479 1108 6 0.004693381939116392 1108 7 0.20887426061866776 1108 8 0.5625256400818834 1108 9 1.3408645328909365 1108 10 1.0541035122997293 1108 11 0.3588005791276636 1108 12 0.49105136826442575 1108 13 0.1291500204593099 1108 14 0.7905870025021464 1108 15 0.5435241631127141 1108 16 0.17381448473894423 1108 17 0.11595307737037786 1108 18 -0.1233315046747272 1108 19 0.23049400814911536 1108 20 0.6371200303534366 1108 21 1.1696448254192653 1108 22 1.121950461391041 1108 23 0.8195300123378892 1108 24 0.8542666561442864 1108 25 0.5181809255933061 1109 1 0.020733711795895005 1109 2 0.07139967537754544 1109 3 -0.26047064162372635 1109 4 0.19830843232086792 1109 5 0.24021380198066922 1109 6 0.05834122844346368 1109 7 0.16243872600310638 1109 8 0.1391175530431532 1109 9 1.2822119593900148 1109 10 1.4687252750819937 1109 11 0.4134731764960442 1109 12 0.16923558164570096 1109 13 0.0867428168264972 1109 14 0.6870505891301697 1109 15 0.1666005854737307 1109 16 0.19045041301258792 1109 17 -0.48216852267921695 1109 18 0.17835936524762275 1109 19 0.3412371540040837 1109 20 1.0119984458021918 1109 21 1.1621287458817342 1109 22 1.2005066351585292 1109 23 0.7990194967078033 1109 24 0.6291308131115692 1109 25 0.6676346087760479 1110 1 0.011155734001068396 1110 2 -0.053230790187548394 1110 3 0.05961309096412748 1110 4 -0.025985316953974547 1110 5 0.06264628711454989 1110 6 -0.24299366971246172 1110 7 -0.2507541377741056 1110 8 0.13405824347252704 1110 9 0.8765070390070484 1110 10 0.6599679787018005 1110 11 -0.3423529022201208 1110 12 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19 1.833978083105876 2203 20 2.201027742959745 2203 21 2.6057637387482764 2203 22 2.493447159893922 2203 23 2.4377491651814545 2203 24 2.1036318534317715 2203 25 2.3134530390863546 2204 1 0.818933373346443 2204 2 0.8717121356924267 2204 3 0.8261127613585266 2204 4 0.8559056774722684 2204 5 1.1965136575637594 2204 6 0.9314279996770303 2204 7 1.3476007003506467 2204 8 1.0791505710613052 2204 9 2.254710135720412 2204 10 2.472252119203283 2204 11 1.4223490576902442 2204 12 1.7759331005690413 2204 13 1.1139711638878005 2204 14 1.8539540129037313 2204 15 1.7571683004673986 2204 16 1.673863731855004 2204 17 1.5355235828248652 2204 18 1.5657729439814219 2204 19 1.681804130669974 2204 20 2.377973348564417 2204 21 2.6237856124777217 2204 22 2.5370027677976417 2204 23 2.690490629568412 2204 24 2.334797331128243 2204 25 2.4201105171407065 2205 1 0.14850875656261664 2205 2 0.1807752549001158 2205 3 0.2917657862930554 2205 4 0.1885756134694141 2205 5 0.4224937533469877 2205 6 -0.09744672249590636 2205 7 -0.367271767626395 2205 8 0.2861328366372753 2205 9 0.8488120200926765 2205 10 0.9893600651002821 2205 11 0.37512233300932607 2205 12 0.450921803751843 2205 13 0.32908047746046165 2205 14 0.3210965526119729 2205 15 -0.44634172255112503 2205 16 -0.5657439714546904 2205 17 -0.5577884143088703 2205 18 -0.07227404733308873 2205 19 0.08902571077747012 2205 20 0.6082824650815439 2205 21 0.6592675516373951 2205 22 0.19423649162476764 2205 23 -0.4690234149447825 2205 24 -0.3926455804706457 2205 25 -0.2466209160306505 2206 1 -0.7928785999831263 2206 2 -2.7032671971486693 2206 3 -0.6086737526438242 2206 4 -1.6342784017552603 2206 5 -0.09401303930485128 2206 6 -0.6004063918438654 2206 7 -0.32739312593822945 2206 8 0.18633764166181166 2206 9 1.3863539352928207 2206 10 0.880502809819177 2206 11 0.6393085065162438 2206 12 0.284920593283438 2206 13 0.4927338464321558 2206 14 1.144798197143448 2206 15 0.3583688306912123 2206 16 0.8799438887076967 2206 17 0.8272113728104529 2206 18 0.31339138437447644 2206 19 0.7834071806387755 2206 20 1.4422635459552282 2206 21 1.9162113864309411 2206 22 1.7839733572953058 2206 23 1.4000975461153864 2206 24 1.1301422596344892 2206 25 1.020938945198779 2207 1 0.3491311497178202 2207 2 0.34532655640084897 2207 3 0.18777849180681372 2207 4 0.2993915771054496 2207 5 0.5815607104267215 2207 6 0.2971144390080731 2207 7 0.2969385290622739 2207 8 0.6084071180392883 2207 9 0.9909762137648741 2207 10 1.537135667599074 2207 11 0.2412582207913445 2207 12 0.4896292964622653 2207 13 0.5913222721733646 2207 14 1.287944420442745 2207 15 0.7316684660030432 2207 16 -0.11959035165414278 2207 17 0.03830039314511424 2207 18 0.5694361876653886 2207 19 0.6490215309053369 2207 20 1.1353357258973418 2207 21 1.4513930571638518 2207 22 1.4946132477821727 2207 23 0.7042123586901177 2207 24 1.0904358425811864 2207 25 0.9860349605880501 2208 1 0.17111007808145118 2208 2 0.3456201273018529 2208 3 0.3533714981111551 2208 4 0.29652306551798446 2208 5 0.3505877240211177 2208 6 0.25794433524511007 2208 7 0.5064936337150527 2208 8 0.4958993839963135 2208 9 1.504690827348572 2208 10 1.610242083419955 2208 11 0.7837606481973829 2208 12 0.9257557834501711 2208 13 0.7530184042758883 2208 14 1.312710416656639 2208 15 0.9340070515699139 2208 16 0.9340328457721121 2208 17 0.7402425946018312 2208 18 0.42023877616082217 2208 19 0.7837099469456703 2208 20 1.5557999114000296 2208 21 2.032548871273878 2208 22 1.6619040551747624 2208 23 1.6852955350373655 2208 24 1.5818877080185203 2208 25 1.1466098293513696 2209 1 0.5302135907787004 2209 2 0.5202479600924441 2209 3 0.5161558848106083 2209 4 0.4886968469288284 2209 5 0.6972727405988017 2209 6 0.5569103528344428 2209 7 0.7633286986597092 2209 8 0.6897152424546249 2209 9 1.6122962562638996 2209 10 1.70241992617931 2209 11 0.636605069012159 2209 12 0.911282614195315 2209 13 0.6930572086148885 2209 14 1.0035614906987114 2209 15 0.8048210934978002 2209 16 0.6666733499700719 2209 17 0.34294302215106853 2209 18 0.6201669641348033 2209 19 0.48925941047845356 2209 20 1.2693843837570142 2209 21 1.8702592306663028 2209 22 1.7153864769199636 2209 23 1.4473355873179976 2209 24 1.0275219429712266 2209 25 1.0012033991290288 2210 1 0.1118094030251342 2210 2 0.2956777131087911 2210 3 0.39938228558004596 2210 4 0.30021622619373645 2210 5 0.05216414115836088 2210 6 0.30328124624985264 2210 7 0.548767923671619 2210 8 0.42277743459195705 2210 9 1.5866230875619998 2210 10 1.6023393503403796 2210 11 0.7679715184373599 2210 12 0.6537524689763003 2210 13 0.48073873238339004 2210 14 1.0032345790985282 2210 15 0.4929458300065974 2210 16 0.5935040093049118 2210 17 0.5306819892837922 2210 18 0.4986121972024691 2210 19 0.7527159405889989 2210 20 1.5333439425335769 2210 21 1.8631835222285638 2210 22 1.6595005624526316 2210 23 1.6629017352453808 2210 24 1.5872671481156644 2210 25 1.5219667342934926 2211 1 0.17383700858743723 2211 2 0.006107685481548941 2211 3 -0.1409619777590052 2211 4 -0.03041369929807658 2211 5 0.16632986576485465 2211 6 -0.18193047904584958 2211 7 0.1998738488725671 2211 8 0.34425139330872057 2211 9 1.0884944865247448 2211 10 1.1239626235248856 2211 11 0.527959125246209 2211 12 -0.17414928336853563 2211 13 -0.31705397884848097 2211 14 0.3424065025588341 2211 15 0.2677490361576673 2211 16 0.17871306988386593 2211 17 0.029913060423147897 2211 18 -0.16910394632911263 2211 19 -0.18356300376765233 2211 20 0.4451463343652726 2211 21 1.3835846388803752 2211 22 1.291069145713056 2211 23 1.1909661708064299 2211 24 0.9351549536039911 2211 25 0.7450333355014678 2212 1 0.662302490005569 2212 2 0.6614922632959406 2212 3 0.5992140701732396 2212 4 0.6815624840939911 2212 5 0.4940035899979591 2212 6 0.6824639213500632 2212 7 0.9255315740944567 2212 8 0.842690465543491 2212 9 -8.65184403345458 2212 10 -1.1866906586347383 2212 11 0.05464017517446962 2212 12 0.8851669684711112 2212 13 0.9506040164956495 2212 14 1.5123901407266054 2212 15 1.1248483918014998 2212 16 1.1279537622540055 2212 17 1.1818538288959957 2212 18 1.2849221957222952 2212 19 1.5287518291826743 2212 20 1.9570994925776617 2212 21 2.397232290835351 2212 22 2.278958748448968 2212 23 2.2616998218075075 2212 24 2.1181959120096536 2212 25 2.0244122437391496 2213 1 0.6738297838499356 2213 2 0.7281985245143971 2213 3 0.5711270149520367 2213 4 0.8421579861529089 2213 5 0.8910300391585544 2213 6 0.6250610599837856 2213 7 1.0659496925613574 2213 8 1.3902131664198514 2213 9 2.175166049957941 2213 10 -8.13177301530246 2213 11 -1.707833428457672 2213 12 -0.21544146780805917 2213 13 0.7130406652127839 2213 14 1.2963002346976786 2213 15 1.0110589292315524 2213 16 1.3290241349707195 2213 17 0.9012617583648961 2213 18 0.8453210448406523 2213 19 1.429073864870567 2213 20 2.18804923389326 2213 21 2.026388398954875 2213 22 2.315736807788003 2213 23 1.802136417731905 2213 24 1.8663389275307125 2213 25 1.570356441817495 2214 1 -1.8694126822186163 2214 2 -2.0916024876779176 2214 3 -1.9595924467456847 2214 4 -1.9979616365754111 2214 5 -1.9002714331211799 2214 6 -1.8767764141362946 2214 7 -9.94389617815408 2214 8 -3.5295562147625716 2214 9 -2.698232270722986 2214 10 -2.335390452615549 2214 11 -2.605501360364734 2214 12 -12.0202695101361 2214 13 -5.2913042853278185 2214 14 -12.755865051706738 2214 15 -6.566488804837924 2214 16 -4.496944924150512 2214 17 -3.6527898864476143 2214 18 -3.3922882259207903 2214 19 -3.7096769977933413 2214 20 -3.6615079319682557 2214 21 -12.767213510407906 2214 22 -15.277321623313906 2214 23 -9.878120081002855 2214 24 -8.654252859908897 2214 25 -15.894236955928077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80886.0 3 3.0 4 1039.0 5 10980.0 6 14750.0 7 11489.0 8 9408.0 9 6802.0 10 6037.0 11 5440.0 12 4721.0 13 4467.0 14 4417.0 15 4384.0 16 4450.0 17 4963.0 18 5823.0 19 7917.0 20 11137.0 21 14162.0 22 16775.0 23 19430.0 24 22011.0 25 24535.0 26 27137.0 27 29823.0 28 32497.0 29 38115.0 30 44153.0 31 53335.0 32 66301.0 33 80284.0 34 97566.0 35 116589.0 36 145922.0 37 200125.0 38 347888.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.97911364722746 19.269694982565085 13.92292325144561 20.82826811876185 2 16.943660765005124 23.85041366638211 36.75191759042109 22.454007978191672 3 18.87508020046467 29.82353041969514 30.674178909070598 20.62721047076959 4 11.905810529522261 18.914682438950095 34.92232319642585 34.25718383510179 5 11.463224705859206 40.71826265428945 31.96331006523487 15.85520257461647 6 27.304620219505164 38.597573435990775 17.874871646466453 16.2229346980376 7 24.847532146760066 36.63404288265465 20.927537629891187 17.5908873406941 8 27.51831722771774 33.09704003758682 19.603814554735813 19.78082817995962 9 27.219750579126075 15.858788436518262 20.29638561863358 36.62507536572208 10 16.469030013904902 32.34803766953544 28.591555642148297 22.59137667441136 11 31.834829953905057 25.07988710769639 22.230860572166222 20.85442236623233 12 24.29903101906737 30.445218433231197 28.00728068460775 17.248469863093682 13 29.440651795477464 22.969533080029368 27.373391914369975 20.21642321012319 14 22.96849366795391 22.83084187178519 30.415589113513548 23.78507534674735 15 24.42936877877589 28.524294703174036 27.613556676006105 19.432779842043974 16 20.347580517214027 28.96258924321752 28.82056163731556 21.869268602252895 17 19.65270192999079 28.169323357705462 30.452552409807154 21.725422302496593 18 18.713929783099562 23.861332981330747 36.33434576384772 21.090391471721965 19 20.10297501716673 24.381902391411682 37.53104925868903 17.98407333273255 20 20.94238559351862 22.632804950628106 37.57255396351024 18.852255492343037 21 21.65285783472691 22.375004839914663 37.08904363920959 18.88309368614883 22 21.327150777854502 22.824977659531257 37.01555455136277 18.832317011251472 23 20.157462823922163 22.832109468713703 37.68146939915334 19.32895830821079 24 20.271900581510838 23.29095913431485 37.53597554900195 18.90116473517236 25 19.58051721402507 22.90617166428685 38.4392828811677 19.074028240520388 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6487.0 1 6487.0 2 6389.5 3 6292.0 4 6292.0 5 6292.0 6 13626.5 7 20961.0 8 20961.0 9 20961.0 10 20813.5 11 20666.0 12 20666.0 13 20666.0 14 16156.5 15 11647.0 16 11647.0 17 11647.0 18 19518.0 19 27389.0 20 27389.0 21 27389.0 22 44053.0 23 60717.0 24 60717.0 25 60717.0 26 88365.5 27 116014.0 28 116014.0 29 116014.0 30 127123.5 31 138233.0 32 138233.0 33 138233.0 34 143648.0 35 149063.0 36 149063.0 37 149063.0 38 146066.5 39 143070.0 40 143070.0 41 143070.0 42 150444.5 43 157819.0 44 157819.0 45 157819.0 46 168310.0 47 178801.0 48 178801.0 49 178801.0 50 171682.5 51 164564.0 52 164564.0 53 164564.0 54 147326.0 55 130088.0 56 130088.0 57 130088.0 58 113710.0 59 97332.0 60 97332.0 61 97332.0 62 82051.5 63 66771.0 64 66771.0 65 66771.0 66 52440.0 67 38109.0 68 38109.0 69 38109.0 70 29786.0 71 21463.0 72 21463.0 73 21463.0 74 15582.5 75 9702.0 76 9702.0 77 9702.0 78 7855.0 79 6008.0 80 6008.0 81 6008.0 82 4324.0 83 2640.0 84 2640.0 85 2640.0 86 1950.0 87 1260.0 88 1260.0 89 1260.0 90 938.0 91 616.0 92 616.0 93 616.0 94 331.0 95 46.0 96 46.0 97 46.0 98 24.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03008070386308584 2 0.013707662519887217 3 0.001396150812210735 4 6.34614005550334E-5 5 0.004442298038852339 6 0.0022211490194261694 7 0.0012057666105456347 8 0.047024897811279755 9 0.007044215461608708 10 0.003680761232191938 11 0.006346140055503341 12 0.00539421904717784 13 0.0032999928288617373 14 0.002919224425531537 15 3.807684033302004E-4 16 6.980754061053675E-4 17 0.0034269156299718043 18 9.519210083255012E-4 19 0.002474994621646303 20 0.8333116506881437 21 0.02030764817761069 22 0.009201903080479844 23 0.009582671483810045 24 0.0024115332210912694 25 6.980754061053675E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1575761.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.17931236041159 #Duplication Level Percentage of deduplicated Percentage of total 1 73.03607373762617 25.693588515940046 2 11.364854783261512 7.9961555270214895 3 4.894895652185771 5.165971893595915 4 2.831089004699525 3.983830576658055 5 1.788603250413069 3.1460916217564407 6 1.1702083499205542 2.4700275041170205 7 0.8382761617701749 2.064298525743993 8 0.6538767780165579 1.8402348335251202 9 0.4584601434481133 1.4515481332044256 >10 2.603095919261427 16.687439455621274 >50 0.2168326415318533 5.290810106528135 >100 0.1293602244194906 8.232762132743082 >500 0.008888784367757878 2.124705683185274 >1k 0.0037824614330884587 2.3493183772962336 >5k 5.673692149632688E-4 1.0997106697156536 >10k+ 0.0011347384299265377 10.403506443347892 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 42764 2.713863333335449 No Hit TATCAACGCAGAGTACTTTTTTTTT 35665 2.2633508507952667 No Hit GGTATCAACGCAGAGTACTTTTTTT 31618 2.0065225627490464 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 16731 1.061772692686264 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 16621 1.0547919386252103 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 12969 0.8230309037982282 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 5846 0.3709953476447253 No Hit ATCAACGCAGAGTACTTTTTTTTTT 5479 0.347705013641028 No Hit GTGGTATCAACGCAGAGTACTTTTT 5204 0.33025312848839383 No Hit GTGGTATCAACGCAGAGTACATGGG 3257 0.20669378160774382 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 3078 0.19533419090839282 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 2816 0.17870730396297407 No Hit CAACGCAGAGTACTTTTTTTTTTTT 2352 0.14926121410543858 No Hit GGTATCAACGCAGAGTACATGGGAT 2220 0.14088430923217418 No Hit GGTCGGGAGTGGGTAATTTGCGCGC 2051 0.1301593325383735 No Hit AACGCAGAGTACTTTTTTTTTTTTT 1910 0.12121127506011381 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 1711 0.10858245634966215 No Hit TCAACGCAGAGTACTTTTTTTTTTT 1686 0.10699592133578634 No Hit GGTATCAACGCAGAGTACATGGGAG 1637 0.10388631270858968 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.34614005550334E-5 0.0 0.0 0.0 0.0 8 6.34614005550334E-5 0.0 0.0 0.0 0.0 9 6.34614005550334E-5 0.0 0.0 0.0 0.0 10 6.34614005550334E-5 0.0 0.0 0.0 0.0 11 1.9038420166510024E-4 0.0 0.0 6.34614005550334E-5 0.0 12 1.9038420166510024E-4 0.0 0.0 6.34614005550334E-5 0.0 13 2.538456022201336E-4 0.0 0.0 1.269228011100668E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 25 0.006117386 18.953875 1 AGGCGTA 35 0.0022118234 16.239477 13 CAAGACT 165 0.0 14.934303 4 TATCACG 130 0.0 14.582682 2 CGACCGT 220 0.0 14.329125 19 TATAACG 140 0.0 14.218114 2 TCGACCG 240 0.0 13.931093 18 ATAACGC 165 0.0 13.785511 3 CGTTTAA 85 2.7801434E-7 13.379206 1 GTTCGAC 255 0.0 13.111618 16 TTTAGAC 80 2.0532934E-6 13.031616 3 GGTATCA 7745 0.0 13.019318 1 TAGTCAA 265 0.0 12.869428 9 GTATAAA 280 0.0 12.861558 1 CCTCTAG 305 0.0 12.739489 1 TTCGACC 270 0.0 12.7369995 17 AGATAGT 300 0.0 12.636719 6 ATCACGC 150 1.8189894E-12 12.636719 3 AAACGCA 210 0.0 12.636719 5 TGCACCG 75 1.5150328E-5 12.636719 5 >>END_MODULE