FastQCFastQC Report
Thu 26 May 2016
SRR1364268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107187
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT109650.5203619802134314No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA107710.5111553934226055No Hit
TATCAACGCAGAGTACTTTTTTTTT74290.35255532612910007No Hit
GTACTTTTTTTTTTTTTTTTTTTTT69110.32797279026493614No Hit
GGTATCAACGCAGAGTACTTTTTTT59660.2831262721343668No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN46430.22034114675156974No Hit
GAGTACTTTTTTTTTTTTTTTTTTT36450.17297942707505315No Hit
AAACAAAAAAAAAAAAAAAAAAAAA31960.1516713988839149No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA30090.14279700852368585No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26620.12632955689267256No Hit
GAACAAAAAAAAAAAAAAAAAAAAA21990.1043571358403407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC2150.015.9089113
TCACGCA2300.015.2844674
TATCACG2350.014.9585532
CTCAACG2800.014.2517324
CGACCGT954.8967195E-914.00170119
GTATCAC3100.013.7906861
TAACGCA2800.013.2337514
TATAACG2300.013.2183692
GTTCGAC1102.741217E-912.957046516
ATAACGC2500.012.9215733
ACATGGT10550.012.7882843
TCGACCG1051.9908839E-812.668509518
GGTATCA38400.012.3948571
ATGCGTA951.0424046E-611.99802913
TGCGTAG1001.925422E-611.402472514
AATGCGT1001.9342679E-611.39812812
GGTCTCA3850.011.3509491
ACATGCG855.3181015E-511.177833
TACAACG1701.0913936E-1111.1772982
GTACAAA5900.011.1104621
Produced by FastQC (version 0.11.5)