FastQCFastQC Report
Thu 26 May 2016
SRR1364268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107187
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT369381.752953107626423No Hit
TATCAACGCAGAGTACTTTTTTTTT292351.3873946640711052No Hit
GTACTTTTTTTTTTTTTTTTTTTTT262071.2436959795215137No Hit
GGTATCAACGCAGAGTACTTTTTTT239901.1384846242882098No Hit
GAGTACTTTTTTTTTTTTTTTTTTT141370.6708944199067287No Hit
ACGCAGAGTACTTTTTTTTTTTTTT120810.5733235825771514No Hit
ATCAACGCAGAGTACTTTTTTTTTT47950.22755455495881474No Hit
GCAGAGTACTTTTTTTTTTTTTTTT46940.2227614350316322No Hit
GTGGTATCAACGCAGAGTACTTTTT43060.2043482614499805No Hit
GTGGTATCAACGCAGAGTACATGGG37630.1785793097622565No Hit
GGTATCAACGCAGAGTACATGGGAT29590.14042417687656578No Hit
GTATAACGCAGAGTACTTTTTTTTT26180.12424146504320689No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT21850.10369274297914709No Hit
GGTATCAACGCAGAGTACATGGGAG21590.10245887052264463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCG601.4967827E-615.8032635
TATCACG2300.015.6662142
TATAACG3850.015.5162972
ATAACGC3850.015.5159293
TAACGCA4100.014.8011044
GGTATCA68300.013.3123471
CAACCGT550.003113071212.06794556
ATCACGC2950.011.8926253
TCACGCA3050.011.5027024
GTATAAC6150.011.2539381
CGACCGT855.0858027E-511.22694219
TCGACCG951.2944194E-511.04967418
AACTGGC1303.0569936E-811.01130217
GTATCAC4450.010.8659311
GTCGGTT1159.091473E-710.713901512
GTTCGAC1002.2824606E-510.4974416
GTGGTAT20000.010.4291631
GTATAAA3950.010.3211511
TTTATAC3700.09.9944963
ACTGACC1252.6338275E-69.8612368
Produced by FastQC (version 0.11.5)