##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364267_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1736464 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.29567557979895 29.0 20.0 31.0 2.0 34.0 2 24.870606589022287 30.0 23.0 31.0 2.0 34.0 3 24.489425637387242 30.0 22.0 31.0 2.0 34.0 4 27.328730684886068 33.0 22.0 35.0 2.0 37.0 5 27.994054008605996 33.0 26.0 35.0 2.0 37.0 6 28.488717301366457 35.0 28.0 35.0 2.0 37.0 7 28.440616678491462 35.0 28.0 36.0 2.0 37.0 8 26.735398487961742 33.0 18.0 35.0 2.0 37.0 9 26.460069428447696 32.0 17.0 37.0 2.0 39.0 10 28.191997069907583 34.0 25.0 37.0 2.0 39.0 11 28.629839144376156 35.0 27.0 39.0 2.0 39.0 12 27.33785439836357 34.0 17.0 38.0 2.0 39.0 13 27.066526573542557 33.0 17.0 38.0 2.0 39.0 14 27.56658473771987 33.0 17.0 39.0 2.0 40.0 15 27.171313082217655 33.0 17.0 38.0 2.0 40.0 16 27.970334541919673 34.0 18.0 39.0 2.0 40.0 17 28.51143933879424 34.0 24.0 39.0 2.0 41.0 18 29.016228957237235 35.0 26.0 39.0 2.0 41.0 19 29.647271121082845 36.0 27.0 40.0 2.0 41.0 20 29.66375461858121 36.0 27.0 40.0 2.0 41.0 21 29.823323144044448 36.0 27.0 40.0 2.0 41.0 22 29.729541182541073 36.0 27.0 40.0 2.0 41.0 23 29.36975255461674 36.0 27.0 40.0 2.0 41.0 24 29.354555579614665 36.0 27.0 40.0 2.0 41.0 25 29.085243920979647 36.0 26.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.17794045048716 1101 2 -0.2013902238587164 1101 3 -2.475460471707052 1101 4 -2.626448301757744 1101 5 -3.0358391085075738 1101 6 -1.3633998151771856 1101 7 -0.9275743754137018 1101 8 -4.305901977128752 1101 9 -5.608914702458129 1101 10 -5.664128791879595 1101 11 -2.6793821025488818 1101 12 -0.12967272415640352 1101 13 0.42687801595268837 1101 14 -6.981690972236699 1101 15 -8.803513776593736 1101 16 -3.951398872647541 1101 17 -1.7427460014595546 1101 18 -6.034120252830771 1101 19 -2.760577518862547 1101 20 -1.6807712133998756 1101 21 -1.5913007636673804 1101 22 -1.421489915171371 1101 23 -6.12227757612872 1101 24 -2.9798060762158443 1101 25 -2.086763195804906 1102 1 1.8412018985017085 1102 2 1.2761376069824664 1102 3 1.6664109465130927 1102 4 1.8084966952780235 1102 5 1.3160349445851054 1102 6 0.7444356370328151 1102 7 0.4842331471473962 1102 8 1.9721067179506235 1102 9 -3.315175818326651 1102 10 -0.6581739799436228 1102 11 0.1525558462028478 1102 12 -3.6202991651023204 1102 13 -5.809973970082488 1102 14 -5.706949519104494 1102 15 -1.0378264042203753 1102 16 0.3502413355073912 1102 17 -4.057402479952408 1102 18 -1.4683143111931969 1102 19 -0.7034139591497492 1102 20 -0.37867648973213264 1102 21 -0.23806316403626226 1102 22 -0.08499034333815914 1102 23 0.11114248842565644 1102 24 0.18304880817912306 1102 25 0.24884710654671593 1103 1 -0.5589784344413502 1103 2 1.1261739427247264 1103 3 1.9868100052443403 1103 4 2.779559602252757 1103 5 2.2291582996237587 1103 6 1.5996881704415102 1103 7 1.6551181243366244 1103 8 2.913327310511601 1103 9 3.856941113552484 1103 10 2.537101989057568 1103 11 2.0525882407667133 1103 12 3.279444946681032 1103 13 3.4586885669982337 1103 14 -2.191882009602409 1103 15 1.8890007164700435 1103 16 2.3976112237509497 1103 17 2.369290256167435 1103 18 2.151531732921086 1103 19 1.7684423743239286 1103 20 1.80007844395033 1103 21 1.7646702834951178 1103 22 1.6153284114801743 1103 23 2.060940430938281 1103 24 1.8885041680856105 1103 25 1.8309266645195272 1104 1 1.8587907294601926 1104 2 1.1632742684510156 1104 3 1.7974654732276356 1104 4 1.9720500674745551 1104 5 1.2591858082984366 1104 6 0.6512158368815548 1104 7 0.5861737413393335 1104 8 1.8146622519403017 1104 9 -3.346195542179327 1104 10 -0.7775143318540998 1104 11 0.10476716650295614 1104 12 1.3981463516867123 1104 13 1.5289640741305348 1104 14 1.445836717702207 1104 15 -3.427859400010565 1104 16 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2.195011851230351 1106 4 2.242406725144079 1106 5 1.4579202458884808 1106 6 0.8402281220907284 1106 7 0.9156375320914236 1106 8 2.299829112178532 1106 9 3.2217621400653726 1106 10 1.3759902274062696 1106 11 0.699657079611768 1106 12 1.817396555384569 1106 13 -4.261828894076817 1106 14 0.17842213193405243 1106 15 2.094340251510033 1106 16 1.4964573960301308 1106 17 1.356655217611813 1106 18 1.008736449024525 1106 19 0.7750785759465906 1106 20 0.5482021954690133 1106 21 0.043437392907907935 1106 22 0.20067692891555566 1106 23 0.518786069115734 1106 24 0.6183112442170966 1106 25 0.6486148686778144 1107 1 2.1495996974661793 1107 2 1.4704361008508542 1107 3 2.1482838241916085 1107 4 2.0611611824100855 1107 5 1.7202128766149052 1107 6 0.9154600561128312 1107 7 0.31075989169008267 1107 8 2.1328629602752187 1107 9 3.4320971973521424 1107 10 1.7871256844555887 1107 11 0.792997969754019 1107 12 2.4084612917815065 1107 13 2.6247334843229275 1107 14 -3.1150256753227517 1107 15 1.2097333692257948 1107 16 1.5852676369986902 1107 17 1.2458897949898464 1107 18 1.2904520084739488 1107 19 1.0790427455932736 1107 20 1.0775149052253852 1107 21 0.699813617032337 1107 22 0.6434637253807942 1107 23 1.1976859015865706 1107 24 1.2675448053202558 1107 25 1.2566573306411755 1108 1 1.7899647751744716 1108 2 1.2439290770893336 1108 3 1.5907153654983617 1108 4 1.8059935663668867 1108 5 1.331766147049727 1108 6 0.6140274344719217 1108 7 0.46304801188215805 1108 8 -3.694163619971782 1108 9 -4.936091385862994 1108 10 -1.5728172840737855 1108 11 -0.12093505588630293 1108 12 1.0393138126583423 1108 13 2.2151584069272374 1108 14 2.432450692698719 1108 15 -3.132791264141428 1108 16 0.2498466113329414 1108 17 0.8825416810180364 1108 18 0.6863116635829662 1108 19 0.574702917715566 1108 20 0.5928263503564395 1108 21 0.3799593641239376 1108 22 0.386724299965973 1108 23 0.6444581714910278 1108 24 0.6298669462363122 1108 25 0.8606792043612934 1109 1 0.8558978574471539 1109 2 0.690700511260065 1109 3 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2.0689728420000755 1110 16 1.69516334552306 1110 17 1.1005033670473878 1110 18 0.6572260474202665 1110 19 -0.15125426078783022 1110 20 -0.15263258554546866 1110 21 -0.456695139377441 1110 22 -0.7054613135136769 1110 23 -0.19088700304145334 1110 24 -0.14351718619909448 1110 25 0.08897868653241758 1111 1 1.0747098919464655 1111 2 0.8163318889098363 1111 3 0.8690934264072183 1111 4 1.4329099870123088 1111 5 -1.5089979414187304 1111 6 -0.5373185692816982 1111 7 -0.7745675267412189 1111 8 1.16885175211414 1111 9 2.5729128611031804 1111 10 1.127953016920479 1111 11 0.7081964695498151 1111 12 1.9589472214294865 1111 13 2.2108446169471527 1111 14 2.421770890977484 1111 15 2.781522343117306 1111 16 1.9188703833802272 1111 17 1.3908500206813592 1111 18 0.8059022633527384 1111 19 0.37191145758927746 1111 20 0.5335734718426401 1111 21 0.22649640304619822 1111 22 -0.0030547899250983335 1111 23 0.44428589136353835 1111 24 0.24905216564549448 1111 25 0.2520866024876156 1112 1 2.5428494116573326 1112 2 1.681482828385466 1112 3 2.3657135510492715 1112 4 2.5130582153286554 1112 5 2.086390291656535 1112 6 1.2145541591972062 1112 7 1.21172886519647 1112 8 -3.0400074063135065 1112 9 2.100156749755403 1112 10 1.3935131797106557 1112 11 1.43781455520271 1112 12 2.4330685070179072 1112 13 2.8725512601920364 1112 14 3.2215287781483006 1112 15 3.8219075963845413 1112 16 2.7361783803954864 1112 17 2.2567776291647377 1112 18 2.031491220902396 1112 19 1.6459803292590607 1112 20 1.619865861462273 1112 21 1.3822003916028578 1112 22 1.1864741591703485 1112 23 1.5020159849026449 1112 24 1.5140914788257867 1112 25 1.50610254487178 1113 1 -0.12117318873740146 1113 2 -0.14064234597881153 1113 3 -6.276006366405316 1113 4 -3.6120558212589593 1113 5 -1.037254105287289 1113 6 -0.9883264344558214 1113 7 -0.35959173173591097 1113 8 1.2488277707030448 1113 9 -5.7287484478380115 1113 10 -2.025888728952747 1113 11 -0.6414193807054858 1113 12 -4.562046615944084 1113 13 -0.1945035027147881 1113 14 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-1.336476591480828 2204 4 1.8187595238136929 2204 5 1.9735711505568574 2204 6 1.8259750613131658 2204 7 1.7630012501332715 2204 8 3.5216418511528396 2204 9 -2.191387153924719 2204 10 0.6092495852271256 2204 11 1.7281413995731327 2204 12 3.4017641283001474 2204 13 3.6362713365508057 2204 14 3.8468766922311026 2204 15 4.168073828213025 2204 16 3.738834219469485 2204 17 2.8193899391428907 2204 18 2.7904734592341214 2204 19 2.192098288302425 2204 20 2.188711316454171 2204 21 1.890293710628498 2204 22 1.8630160932790432 2204 23 2.400852840651652 2204 24 2.3175919871528414 2204 25 2.2148760713241167 2205 1 -2.695936133359357 2205 2 -2.9597675144881848 2205 3 0.1202256930507346 2205 4 -1.4458582361873695 2205 5 -2.103223488794434 2205 6 -0.6388217229060835 2205 7 -0.49573095796835887 2205 8 -4.03688119429345 2205 9 1.2255813566375195 2205 10 0.9831880134458331 2205 11 0.7531910043983245 2205 12 2.4032221821557265 2205 13 -3.5532900208387694 2205 14 0.1810574829229843 2205 15 2.4585013091435712 2205 16 2.0063500231342033 2205 17 1.8423228178960542 2205 18 0.63596034711248 2205 19 0.8008028774261575 2205 20 0.7926167635250856 2205 21 0.7026169087635274 2205 22 0.7246115479843951 2205 23 0.8654438848771946 2205 24 0.5500838844767948 2205 25 1.123913609365573 2206 1 -7.244256052262784 2206 2 -5.330184774775464 2206 3 -2.063795871456996 2206 4 -0.8546200079435415 2206 5 -0.7039007193531859 2206 6 -1.1514390690982488 2206 7 -1.5812534138156806 2206 8 -2.6921914680186205 2206 9 -5.350122351779756 2206 10 -2.231627829927035 2206 11 -1.4966008969642388 2206 12 -7.328580947104157 2206 13 -5.967070285744832 2206 14 -7.1763174895930355 2206 15 -2.1110671636573777 2206 16 -5.710904058849231 2206 17 -2.5891449620294047 2206 18 -1.751608602516427 2206 19 -1.8589338052355622 2206 20 -1.8855031857832358 2206 21 -1.8522099313620402 2206 22 -1.7766289316543897 2206 23 -1.45128766698501 2206 24 -1.2872538309820705 2206 25 -1.5606467833822144 2207 1 -6.811273461323072 2207 2 -4.9078482346946295 2207 3 -0.9353443726358464 2207 4 0.19359330040943945 2207 5 0.5324484301921153 2207 6 0.14159341237699508 2207 7 0.21898909207855866 2207 8 1.5202512736452398 2207 9 -1.5713624578760879 2207 10 -0.27575611386101784 2207 11 0.03284409714152048 2207 12 -4.139739481122348 2207 13 -4.16482573791728 2207 14 -0.7004390372521812 2207 15 -4.218126779689712 2207 16 -0.5527843215639514 2207 17 0.14781224157450268 2207 18 0.3212057920608693 2207 19 0.019375074016711125 2207 20 0.013666673597029444 2207 21 -0.13230792311010475 2207 22 -0.08524378514040976 2207 23 -0.1301055560048816 2207 24 -0.2576872681663076 2207 25 0.12114411843657891 2208 1 -3.4420579354530396 2208 2 -3.6498317002012506 2208 3 -0.9214754166764969 2208 4 0.08049900182613001 2208 5 -2.4935830989116745 2208 6 -0.7978996773981812 2208 7 -0.4773336673917221 2208 8 -4.744078107321805 2208 9 -7.6509210889201285 2208 10 -3.4908968206673023 2208 11 -1.1573423249404016 2208 12 0.39820564129835034 2208 13 1.200050012775666 2208 14 1.3410571515374734 2208 15 1.6784485205874695 2208 16 -4.177152199605217 2208 17 -6.0584771216838895 2208 18 -2.230041994357361 2208 19 -1.674451053947564 2208 20 -0.8024981516262564 2208 21 -0.8053363916964891 2208 22 -0.5099488975518653 2208 23 -0.3835973368795358 2208 24 -0.32727823675269363 2208 25 -0.11149533721479088 2209 1 1.028584721407853 2209 2 0.9685269776933865 2209 3 -1.8872883022002576 2209 4 0.44362757211163384 2209 5 0.8161468778288139 2209 6 0.6594109324573161 2209 7 0.4663910074322821 2209 8 1.827803791408602 2209 9 -3.4227358360039837 2209 10 -0.34003804929506387 2209 11 0.4455600571353422 2209 12 -4.004263442351881 2209 13 0.3801285986597982 2209 14 1.707413719930397 2209 15 2.465462963900034 2209 16 -2.5485048961700656 2209 17 0.056682433882194516 2209 18 0.6079938095153032 2209 19 0.4946800438962278 2209 20 0.6048285325713856 2209 21 0.6502661230746369 2209 22 0.41892906008665065 2209 23 1.0189472899945002 2209 24 0.4440487142247953 2209 25 0.6328730534072307 2210 1 0.023597862206340636 2210 2 -2.8776092641063933 2210 3 -0.6661531961652578 2210 4 -2.7107265525317388 2210 5 -2.7985809815725915 2210 6 -1.1515867346818283 2210 7 -0.35715061380379254 2210 8 -4.71506735380574 2210 9 -4.004683536307457 2210 10 -1.2747389569982737 2210 11 -0.42260320678004604 2210 12 1.0719333129953021 2210 13 -6.890224022231006 2210 14 -1.3711130568600218 2210 15 -4.95030815223873 2210 16 -1.1876250078384594 2210 17 -0.30933849371961486 2210 18 -0.05045474715481646 2210 19 -0.2843810534342701 2210 20 -0.1539620028735591 2210 21 -0.4515787564434355 2210 22 -0.33216780474825214 2210 23 0.0011873361519789682 2210 24 -0.06665785640160138 2210 25 0.04478995176991063 2211 1 0.2598923857339557 2211 2 0.09508854099918196 2211 3 -3.262165812099095 2211 4 -2.7503125207250534 2211 5 -0.6259121599268234 2211 6 -0.8163218247379795 2211 7 -0.9059636374121389 2211 8 -4.405432623884494 2211 9 0.407134987991558 2211 10 0.0527276083103736 2211 11 -5.5509696608639665 2211 12 -0.29995362796589475 2211 13 0.6756443939826902 2211 14 1.2605850935803922 2211 15 -3.774314641842807 2211 16 -0.6594883471815152 2211 17 -5.823792382548707 2211 18 -1.9784134706716685 2211 19 -1.1738495180845412 2211 20 -0.7730540301236175 2211 21 -0.630134528020136 2211 22 -0.46843911908368696 2211 23 -0.29519180562367 2211 24 -0.34451309158808385 2211 25 -0.16964280211846727 2212 1 1.5936561288015696 2212 2 0.8439359815086327 2212 3 1.5543416292817014 2212 4 1.8118753993228296 2212 5 -1.53384131892901 2212 6 -0.08479311287068825 2212 7 0.49073086261227417 2212 8 -1.5474836256836184 2212 9 2.307819988592435 2212 10 1.4379916889513815 2212 11 1.2447737272150263 2212 12 2.7573777088744578 2212 13 2.7149716214627126 2212 14 2.9785053139782462 2212 15 3.6624293085173854 2212 16 2.7635283981548113 2212 17 2.0003606795738627 2212 18 1.7833547414057094 2212 19 1.3474833953653835 2212 20 1.1208941224624809 2212 21 0.9135089313162297 2212 22 0.9444390208679003 2212 23 1.150258877520205 2212 24 1.1729668945054712 2212 25 1.404369689705355 2213 1 2.1820505881454473 2213 2 1.656147830309461 2213 3 2.2104385382390532 2213 4 2.3827145698288987 2213 5 1.4881153353433838 2213 6 -0.5129908578601281 2213 7 0.7397249874178868 2213 8 -5.616704981465144 2213 9 0.765152771069129 2213 10 0.9327010611185393 2213 11 1.0115294507801167 2213 12 2.5623212813469927 2213 13 -1.9346425306400938 2213 14 0.8863501082915661 2213 15 -3.267088349599767 2213 16 0.08220954597489083 2213 17 1.0675636213782624 2213 18 1.2698042691663503 2213 19 0.9180415192333911 2213 20 1.1162548277262196 2213 21 0.9714034934884239 2213 22 1.0021172124066062 2213 23 1.2921836406427047 2213 24 1.3170004873529066 2213 25 1.5208392840130287 2214 1 2.896521235078726 2214 2 2.21939829828888 2214 3 1.7295469317119796 2214 4 2.434881250184553 2214 5 2.2639148844649775 2214 6 1.0456184311176244 2214 7 1.4295438147172383 2214 8 2.8959221881879706 2214 9 3.818346125332308 2214 10 2.3683227841899566 2214 11 2.395900225327427 2214 12 3.5309712572584644 2214 13 1.9900210574350439 2214 14 3.372125617920233 2214 15 4.460254029905492 2214 16 3.2453078823340995 2214 17 2.5840149214845454 2214 18 2.232850750831485 2214 19 2.2155936834619894 2214 20 1.823065567899878 2214 21 1.7143304764401215 2214 22 1.582571057438301 2214 23 -4.266603400832469 2214 24 -0.5984659413297244 2214 25 0.7959768583166387 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 196590.0 3 6.0 4 1234.0 5 9083.0 6 14700.0 7 12865.0 8 10139.0 9 8054.0 10 7330.0 11 6861.0 12 6252.0 13 6242.0 14 6160.0 15 5789.0 16 5369.0 17 5437.0 18 7043.0 19 10067.0 20 15084.0 21 19994.0 22 24909.0 23 29221.0 24 33877.0 25 38866.0 26 42582.0 27 46611.0 28 50034.0 29 57383.0 30 62415.0 31 68774.0 32 85709.0 33 106707.0 34 128010.0 35 144846.0 36 161810.0 37 156783.0 38 143628.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.637383237984416 19.092176771804425 14.63952108526917 20.630918904941982 2 14.885329401978877 24.066827638237655 39.676586833156605 21.371256126626864 3 15.488251834397133 32.32631747265073 30.663280827425528 21.52214986552661 4 9.399357456999178 19.463149662150844 38.26844942890843 32.86904345194155 5 10.061258810536446 42.84087084384286 32.76737297211168 14.330497373509013 6 26.786224722254463 39.412869699389795 19.46808676505491 14.332818813300834 7 25.965240931990213 37.54743915199701 20.41304111816651 16.07427879784627 8 24.17832879552128 35.408850610222636 22.549336074306552 17.86348451994953 9 25.270907398295666 19.529010365052198 22.561486912591217 32.63859532406092 10 15.070253276763435 34.568850867634175 29.025624500841097 21.33527135476129 11 31.0473702415951 28.11510545423887 22.32316355209537 18.514360752070655 12 21.653227971964974 31.80688702545662 28.923231685098553 17.616653317479848 13 25.936228476128985 26.561054512318083 26.87167736339582 20.63103964815711 14 21.003071983739275 26.0832207324256 28.540403515458085 24.373303768377045 15 21.16675318735878 32.06120006028538 26.52453020419076 20.24751654816508 16 21.107777947804397 32.71473158732038 25.33083490447552 20.84665556039971 17 20.04239509782862 32.129617439386905 26.535912840021158 21.292074622763316 18 19.678629750336647 30.24774623911231 29.48679156378553 20.58683244676551 19 21.42655464280992 30.232352691534636 29.202670141862114 19.13842252379333 20 22.86456341582048 28.921355116810254 28.530678216946704 19.683403250422565 21 24.011726642425753 28.532847360206215 27.670037181332763 19.785388816035265 22 23.197961365026185 28.959772992244247 27.77490014418965 20.067365498539914 23 21.578854365052326 29.31328196913297 28.676561455136785 20.431302210677917 24 21.565449083607728 29.443743135286965 28.725655172254488 20.265152608850816 25 21.094893208615378 28.82316390771118 29.393010543433196 20.68893234024025 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15167.0 1 15167.0 2 15029.5 3 14892.0 4 14892.0 5 14892.0 6 17870.0 7 20848.0 8 20848.0 9 20848.0 10 19837.5 11 18827.0 12 18827.0 13 18827.0 14 19758.0 15 20689.0 16 20689.0 17 20689.0 18 28209.0 19 35729.0 20 35729.0 21 35729.0 22 50239.5 23 64750.0 24 64750.0 25 64750.0 26 86303.0 27 107856.0 28 107856.0 29 107856.0 30 124047.0 31 140238.0 32 140238.0 33 140238.0 34 153404.5 35 166571.0 36 166571.0 37 166571.0 38 176141.0 39 185711.0 40 185711.0 41 185711.0 42 195891.5 43 206072.0 44 206072.0 45 206072.0 46 206819.5 47 207567.0 48 207567.0 49 207567.0 50 192990.5 51 178414.0 52 178414.0 53 178414.0 54 157042.5 55 135671.0 56 135671.0 57 135671.0 58 114890.0 59 94109.0 60 94109.0 61 94109.0 62 76435.0 63 58761.0 64 58761.0 65 58761.0 66 45428.5 67 32096.0 68 32096.0 69 32096.0 70 24603.0 71 17110.0 72 17110.0 73 17110.0 74 12550.5 75 7991.0 76 7991.0 77 7991.0 78 6092.0 79 4193.0 80 4193.0 81 4193.0 82 3042.0 83 1891.0 84 1891.0 85 1891.0 86 1410.0 87 929.0 88 929.0 89 929.0 90 633.5 91 338.0 92 338.0 93 338.0 94 187.5 95 37.0 96 37.0 97 37.0 98 22.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22344258216697843 2 0.23421159321471682 3 0.23904900994204314 4 0.3004957200379622 5 0.24791760727547477 6 0.2737171631545486 7 0.27481134074763425 8 0.27175916114586884 9 0.2808005233624193 10 0.2764814012844493 11 0.27440822268702375 12 0.2748689290420072 13 0.2753872236913636 14 0.26974357084281625 15 0.2707225718471561 16 0.2711832782021395 17 0.26836145177786586 18 0.27020427719779966 19 0.26323609357867483 20 0.3090187876051562 21 0.2695708059596974 22 0.271643984557123 23 0.27515687051387183 24 0.2753872236913636 25 0.2746961641588884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1736464.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.51632638985402 #Duplication Level Percentage of deduplicated Percentage of total 1 89.11727376404922 60.1687094242752 2 5.880936963799912 7.941185190521438 3 1.936944714815707 3.923261746937999 4 0.9453890595968212 2.5531678525254446 5 0.5542025802411951 1.8708861146831888 6 0.31273098384341697 1.2668668306435378 7 0.21515830792336652 1.0168688980269907 8 0.155299274561872 0.8388189207541521 9 0.11579794057918849 0.7036426396275669 >10 0.6645149554808849 8.29248542367591 >50 0.06389993205710053 2.9962922721961633 >100 0.033836172078160524 4.08043860923508 >500 0.002562989983030477 1.1670641797538048 >1k 0.0011960619920808892 1.8580852282117983 >5k 1.7086599886869847E-4 0.7392425506709219 >10k+ 8.543299943434924E-5 0.5829841182608464 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAA 10107 0.5820448912272296 No Hit GTATCAACGCAGAGTACTTTTTTTT 7537 0.4340429746887929 No Hit TATCAACGCAGAGTACTTTTTTTTT 5279 0.30400860599471113 No Hit GGTATCAACGCAGAGTACTTTTTTT 4481 0.2580531470851109 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 4294 0.2472841360373725 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3564 0.2052446811451317 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 3451 0.19873720388099034 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 2985 0.17190105870320377 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 2205 0.12698218909231634 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2080 0.11978365229569976 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2018 0.11621317804457795 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.758829437293258E-5 0.0 2 0.0 0.0 0.0 5.758829437293258E-5 0.0 3 0.0 0.0 0.0 1.1517658874586517E-4 0.0 4 0.0 0.0 0.0 1.1517658874586517E-4 0.0 5 0.0 0.0 0.0 1.1517658874586517E-4 0.0 6 0.0 0.0 0.0 1.1517658874586517E-4 0.0 7 0.0 0.0 0.0 1.1517658874586517E-4 0.0 8 0.0 0.0 0.0 1.1517658874586517E-4 0.0 9 0.0 0.0 0.0 1.1517658874586517E-4 0.0 10 0.0 0.0 0.0 1.1517658874586517E-4 0.0 11 0.0 0.0 0.0 1.1517658874586517E-4 0.0 12 0.0 0.0 0.0 1.1517658874586517E-4 0.0 13 0.0 0.0 0.0 1.1517658874586517E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCCGA 85 2.69496E-7 13.413661 19 CCTTACG 50 0.00149579 13.304571 2 ATAACGC 155 1.8189894E-12 12.262631 3 CTCAACG 165 0.0 12.095413 4 GCGCTCG 55 0.003064777 12.092968 14 ACATGGT 950 0.0 12.004471 3 TAACGCA 200 0.0 11.879424 4 TAATACC 185 0.0 11.81521 4 CGACCGT 65 8.0147234E-4 11.693961 19 GGTATCA 3260 0.0 11.48554 1 TTGCGCG 390 0.0 11.450336 18 GGTCTCA 200 0.0 11.403918 1 TACATGT 1195 0.0 11.3721075 2 CGGGATT 70 0.001486923 10.861188 4 TATAACG 175 2.0008883E-11 10.860874 2 TGACCGA 175 2.0008883E-11 10.858678 19 AAGTGCC 70 0.0014941064 10.854916 7 ACTCATA 290 0.0 10.808127 7 GTATCAA 7905 0.0 10.783591 1 CATGGTA 885 0.0 10.738462 4 >>END_MODULE