FastQCFastQC Report
Thu 26 May 2016
SRR1364267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364267_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736464
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT281061.618576601645643No Hit
TATCAACGCAGAGTACTTTTTTTTT238971.3761874706299697No Hit
GGTATCAACGCAGAGTACTTTTTTT208341.1997945249656774No Hit
GTACTTTTTTTTTTTTTTTTTTTTT149430.8605418828147314No Hit
ACGCAGAGTACTTTTTTTTTTTTTT101840.5864791898939454No Hit
GAGTACTTTTTTTTTTTTTTTTTTT92110.530445779469082No Hit
ATCAACGCAGAGTACTTTTTTTTTT36680.2112338637599167No Hit
GCAGAGTACTTTTTTTTTTTTTTTT35750.20587815238323398No Hit
GTGGTATCAACGCAGAGTACTTTTT35490.2043808567295377No Hit
GTGGTATCAACGCAGAGTACATGGG30650.17650812225303836No Hit
GGTATCAACGCAGAGTACATGGGAT26630.15335762791511945No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA25720.1481170931271826No Hit
GGTATCAACGCAGAGTACATGGGAG19430.11189405596660801No Hit
GGTATCAACGCAGAGTACATGGGAA18470.10636557970680648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTG859.567884E-1015.71853414
TATCACG1900.014.9704092
ACTGCGT400.005350062614.2214798
TATAACG2200.013.3599582
GACACGC500.001527113113.26802911
CGACCGT655.21494E-513.21661519
ATAACGC2250.013.061943
TAATACC1550.012.8448364
AAGTTCG751.41110595E-512.72452714
ATCACGC2200.012.4969453
GGTATCA61200.012.1596681
GGTATCG951.0662025E-611.9752933
TCACGCA2250.011.7982224
GTAATAC658.1641786E-411.6682333
TCGACCG751.98435E-411.454418
GTCGGTT1105.1258394E-711.20028612
GTTCGAC700.001433464810.90895316
GTACTAG700.001518147310.8341651
TGGTCGG1159.091E-710.71362410
GGTCGGT1159.094365E-710.71331611
Produced by FastQC (version 0.11.5)