FastQCFastQC Report
Thu 26 May 2016
SRR1364266_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364266_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896733
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN34690.18289342780454604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGT500.001502102413.2972426
TTCGCGC604.0898728E-412.66772617
CGCGGTT1352.7284841E-1112.66738918
GTGCACG2050.012.50960211
CTACGCC1155.295078E-912.3962734
TGACCGA2850.012.33436319
GGTATCA12650.012.1699191
CGCGGGT951.0387776E-612.0013215
TTGACCG3050.011.83674118
ACATGGT7750.011.7724593
CGGTACT657.997898E-411.6969954
AGACCCG2050.011.5829645
CGGTTTG855.3166543E-511.17799514
GCCCCCG2650.011.11384218
CTACGGA600.005858949411.0877774
GCATACG803.7603037E-410.6909361
ACGGCCC2400.010.68867615
CCTACGC909.4780655E-510.5597883
TAAGGCT2650.010.4003944
CCTATAA2650.010.39956951
Produced by FastQC (version 0.11.5)