##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364266_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1896733 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.31185411968896 30.0 25.0 31.0 10.0 33.0 2 26.00265825501006 30.0 25.0 31.0 10.0 34.0 3 25.682690183594634 30.0 25.0 31.0 10.0 34.0 4 28.86253626630633 33.0 27.0 35.0 10.0 37.0 5 29.25751120479266 35.0 28.0 35.0 10.0 37.0 6 29.982855256907534 35.0 30.0 35.0 10.0 37.0 7 30.079594755824886 35.0 30.0 36.0 10.0 37.0 8 28.366046776219953 35.0 26.0 35.0 8.0 37.0 9 28.27748660459854 33.0 22.0 38.0 8.0 39.0 10 30.032277078534513 35.0 28.0 39.0 8.0 39.0 11 30.583199638536367 35.0 30.0 39.0 7.0 39.0 12 29.15569929979602 35.0 27.0 39.0 2.0 39.0 13 28.91051718929338 34.0 26.0 38.0 2.0 39.0 14 29.425679840019654 34.0 25.0 39.0 2.0 41.0 15 28.882186369931876 34.0 24.0 39.0 2.0 41.0 16 29.743682426572427 34.0 27.0 39.0 2.0 41.0 17 30.299677392653578 36.0 27.0 39.0 2.0 41.0 18 30.84892918507771 36.0 29.0 40.0 2.0 41.0 19 31.514719256743042 37.0 30.0 40.0 2.0 41.0 20 31.548043926056014 37.0 30.0 40.0 2.0 41.0 21 31.712548893281237 37.0 30.0 40.0 2.0 41.0 22 31.601282310161736 37.0 30.0 40.0 2.0 41.0 23 31.18141773249055 37.0 30.0 40.0 2.0 41.0 24 31.158359663695418 37.0 30.0 40.0 2.0 41.0 25 30.842836603781343 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.9541932759753031 1101 2 -1.0259474601877088 1101 3 -3.425326005488941 1101 4 -3.7739750845975593 1101 5 -4.436037421571399 1101 6 -2.3778692190866977 1101 7 -1.7574295030855467 1101 8 -5.793313523552996 1101 9 -7.028023818745606 1101 10 -7.133034390583369 1101 11 -3.9696848705093046 1101 12 -1.1711550191397357 1101 13 -0.4602573605847766 1101 14 -8.31052150726546 1101 15 -10.024354945047708 1101 16 -4.831507774659141 1101 17 -2.7652138593074334 1101 18 -7.616790563934476 1101 19 -3.9368317176141545 1101 20 -2.8028034575794933 1101 21 -2.7993136884700576 1101 22 -2.5542981171481607 1101 23 -7.876457730570113 1101 24 -4.478263007864577 1101 25 -3.3405559274211356 1102 1 1.5486372419098693 1102 2 1.0323605175511688 1102 3 1.5260520814693272 1102 4 1.5735192363928832 1102 5 1.1548863822967803 1102 6 0.4636670670019569 1102 7 0.37354703539585543 1102 8 2.0144105416493012 1102 9 -3.7470264573599827 1102 10 -0.9600327889543294 1102 11 -0.1541010811991086 1102 12 -4.549896568517646 1102 13 -6.737204105909122 1102 14 -6.513975173909678 1102 15 -1.2108347659817191 1102 16 0.19099705196918748 1102 17 -4.297425785455111 1102 18 -1.7723131701677843 1102 19 -1.0748597093113652 1102 20 -0.6675642280756122 1102 21 -0.4895895099308838 1102 22 -0.44059203106770184 1102 23 -0.006225394679791663 1102 24 -0.23015131484237727 1102 25 0.06708417116625043 1103 1 -0.7523451912162358 1103 2 1.065889038372223 1103 3 1.9936756738063153 1103 4 2.760791174389169 1103 5 2.446401746974182 1103 6 1.7354818036572794 1103 7 1.716319077956868 1103 8 3.1956731765536404 1103 9 4.4229667934622725 1103 10 2.64326432696042 1103 11 2.3292213203674663 1103 12 3.5097702819294483 1103 13 3.640562898533293 1103 14 -2.7782404958106 1103 15 1.7736877534578142 1103 16 2.322080976251648 1103 17 2.397460207307738 1103 18 2.3480137371878698 1103 19 1.8936621890770695 1103 20 1.8371345304559163 1103 21 1.7090852113882775 1103 22 1.6961958989943362 1103 23 2.0238043270512946 1103 24 1.6853727445503885 1103 25 1.9185477604613546 1104 1 1.8820501159318788 1104 2 1.2198856451778184 1104 3 1.697833937515142 1104 4 2.1566537180045238 1104 5 1.392073823545136 1104 6 0.7978189463314997 1104 7 0.5525526321679521 1104 8 2.1510686757693662 1104 9 -3.5704764985765216 1104 10 -0.7469794388451412 1104 11 0.03461281817468986 1104 12 1.7559865500558267 1104 13 1.8330722618291766 1104 14 1.5830586814998142 1104 15 -3.690696509802855 1104 16 -4.404153858410151 1104 17 -1.090518761289207 1104 18 -0.4573985679435353 1104 19 -0.24017852285352603 1104 20 -0.11636750225959958 1104 21 -0.4043667236928705 1104 22 -0.5535904257919846 1104 23 -0.5727416571351434 1104 24 -6.992576293511011 1104 25 -3.728633647394208 1105 1 -2.3513123954347037 1105 2 0.0061040808870913565 1105 3 1.1696852588572995 1105 4 1.819061386522172 1105 5 1.500565294358399 1105 6 1.1075843035510324 1105 7 0.3434073081550082 1105 8 2.8422888232013257 1105 9 3.237466226270481 1105 10 1.8117488133657993 1105 11 1.6851810023477611 1105 12 2.1831944858177366 1105 13 2.485171961179386 1105 14 2.990212770527627 1105 15 3.5613478175978877 1105 16 3.035113474543536 1105 17 1.6516460664153385 1105 18 1.915586303412006 1105 19 0.8482618366662606 1105 20 1.03424577523759 1105 21 0.8652935097148173 1105 22 0.8481525472340827 1105 23 1.376630642135325 1105 24 1.4520475295242186 1105 25 1.5762921351283161 1106 1 2.3016657721159923 1106 2 1.5851712614166438 1106 3 2.1839261622555455 1106 4 2.4001718662020473 1106 5 1.508795050838284 1106 6 0.9425917420575018 1106 7 0.8340869461112774 1106 8 2.706273660783708 1106 9 3.6359730118591216 1106 10 1.614945144049976 1106 11 0.9277110223647504 1106 12 2.086589877019641 1106 13 -4.393790823092836 1106 14 0.2672805383386532 1106 15 2.3765710643971296 1106 16 1.8914728975285655 1106 17 1.5238437681513162 1106 18 1.1791071819070993 1106 19 1.1115563075641433 1106 20 0.734987625698647 1106 21 0.2869292516585631 1106 22 0.43901796716302854 1106 23 0.9179701985680495 1106 24 0.7569677179047538 1106 25 0.9933515693917023 1107 1 2.1787820873894503 1107 2 1.613932995588268 1107 3 2.3033437281628757 1107 4 2.444299052223574 1107 5 2.131470859263736 1107 6 1.045910976476332 1107 7 0.563741074563751 1107 8 2.4152746017967104 1107 9 4.042145995446148 1107 10 2.2512630703733407 1107 11 1.0487031698503273 1107 12 2.70546538790607 1107 13 2.9892474818430195 1107 14 -3.343904920397044 1107 15 1.3613242031493336 1107 16 1.6016364377390886 1107 17 1.2861023433057923 1107 18 1.5597700759530824 1107 19 1.1905735513574598 1107 20 1.2370353700694103 1107 21 0.7545442489825618 1107 22 0.6319197198190203 1107 23 1.1964377269359687 1107 24 1.3026814816637007 1107 25 1.306143879373824 1108 1 1.7579113686477328 1108 2 1.1734874780081412 1108 3 1.4336349976485465 1108 4 1.8705284464193106 1108 5 1.1960083218416493 1108 6 0.4302155748705623 1108 7 0.08322802337746538 1108 8 -4.955998880259614 1108 9 -6.145563363574368 1108 10 -2.2159853312931723 1108 11 -0.5623775527029196 1108 12 0.6140529478848364 1108 13 2.115663755399897 1108 14 2.3772516190161923 1108 15 -3.7589998736551813 1108 16 -0.3474502477006034 1108 17 0.4050378087480766 1108 18 0.2842780321900378 1108 19 0.3000220063677723 1108 20 0.21861520928097633 1108 21 -0.20759784799224335 1108 22 -0.17094059952198037 1108 23 0.03219295106356057 1108 24 -0.0912101247857926 1108 25 0.3000000993404299 1109 1 0.6492944436399597 1109 2 0.45715289368662937 1109 3 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15 2.684933369696328 1110 16 2.147584082321771 1110 17 1.4409096841547182 1110 18 0.9946127594762189 1110 19 0.2595696478318743 1110 20 0.19030597457724951 1110 21 -0.06877868509537066 1110 22 -0.28684365388614097 1110 23 0.18026620885638778 1110 24 0.47405901697640473 1110 25 0.6475412906895848 1111 1 1.2788201957636574 1111 2 1.0905309658813493 1111 3 1.1917888443338427 1111 4 2.0675386335460146 1111 5 -1.9057617302857643 1111 6 -0.4235076144013483 1111 7 -0.7263101878473712 1111 8 1.8682595390603467 1111 9 3.3691934615241728 1111 10 1.704046069514476 1111 11 1.310412213880955 1111 12 2.6737118915879208 1111 13 2.856393953718971 1111 14 3.192666686567655 1111 15 3.321740100712919 1111 16 2.459447393957106 1111 17 1.7983800775514283 1111 18 1.202823680811889 1111 19 0.6275106671872308 1111 20 0.7041297299954898 1111 21 0.48278241613380146 1111 22 0.3160154566194855 1111 23 0.7534278767759517 1111 24 0.634740967333606 1111 25 0.7042215907578857 1112 1 2.463443543716423 1112 2 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-4.234296780885806 2211 16 -0.5281966789887669 2211 17 -6.271508575939528 2211 18 -2.0554258144118904 2211 19 -0.9165974315472631 2211 20 -0.30278489762618577 2211 21 -0.3161963362153237 2211 22 -0.12314590239134304 2211 23 0.2498312622045198 2211 24 0.21826126726881157 2211 25 0.5178996819913131 2212 1 1.614067188334129 2212 2 0.8219511098928329 2212 3 1.7172426551146955 2212 4 1.9759561071931095 2212 5 -2.040840181793733 2212 6 -0.27447531151863913 2212 7 0.3269225802175022 2212 8 -2.211882814698903 2212 9 2.1097007764067044 2212 10 1.4301712590233855 2212 11 1.0954361608772274 2212 12 2.5698819857487543 2212 13 2.7043688283110363 2212 14 3.066465048069297 2212 15 3.429185240367776 2212 16 2.571475736303192 2212 17 1.84037691632372 2212 18 1.574610606726857 2212 19 1.1120464344779393 2212 20 0.9984163866999545 2212 21 0.7071140012690336 2212 22 0.6817590737827821 2212 23 0.8591964379485617 2212 24 0.9832253886227384 2212 25 1.32816143639241 2213 1 2.335333908854107 2213 2 1.807191987857447 2213 3 2.2820266930169026 2213 4 2.5273035519955016 2213 5 1.2897030563936518 2213 6 -0.7100666967734277 2213 7 0.5083914665471276 2213 8 -6.516291404044683 2213 9 0.3649021650913937 2213 10 0.5389056453325409 2213 11 0.6732959245511339 2213 12 2.2524990729828147 2213 13 -2.490909436641978 2213 14 0.8811551813371885 2213 15 -4.07054098305208 2213 16 -0.2370304165629058 2213 17 0.8368245125555731 2213 18 1.0255404164684947 2213 19 0.9056912814360984 2213 20 1.1023980506539282 2213 21 0.8801402137769045 2213 22 0.835307047303683 2213 23 1.345791740675125 2213 24 1.3333978947330145 2213 25 1.4867455313261182 2214 1 2.1755727608399162 2214 2 1.321708432894816 2214 3 0.8158041844526451 2214 4 1.540325415278236 2214 5 1.5281312915925263 2214 6 0.2504975113526342 2214 7 0.3662752096184896 2214 8 1.8344490140615441 2214 9 2.951821075106441 2214 10 1.2608284077536354 2214 11 1.4205450592706583 2214 12 2.5189566893483857 2214 13 0.6057842685462198 2214 14 2.221697475302335 2214 15 3.438265721174492 2214 16 1.9557562939433275 2214 17 1.2670346844254503 2214 18 1.0838265750448315 2214 19 0.7959636258830827 2214 20 0.41541215571653467 2214 21 0.3161482497188288 2214 22 0.2762336823696536 2214 23 -6.872135257284409 2214 24 -2.8035955476033116 2214 25 -0.9796616566086165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 144471.0 3 8.0 4 1212.0 5 6518.0 6 8554.0 7 8331.0 8 7253.0 9 6349.0 10 5837.0 11 5618.0 12 5283.0 13 5071.0 14 4957.0 15 4985.0 16 5083.0 17 5476.0 18 6621.0 19 8847.0 20 12863.0 21 18199.0 22 24130.0 23 30457.0 24 37216.0 25 43887.0 26 50358.0 27 55474.0 28 61211.0 29 70523.0 30 76684.0 31 84580.0 32 104841.0 33 129108.0 34 153396.0 35 172554.0 36 188206.0 37 181812.0 38 160760.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.766845674104324 16.097884401522574 14.043569336105174 25.091700588267923 2 16.569286988426455 20.180894772882905 37.98039936763972 25.26941887105092 3 17.156388738294595 23.717914168650815 30.593574009433016 28.532123083621574 4 10.862840936008935 15.36194841122128 36.903044736218746 36.87216591655105 5 12.773927830486297 36.459206353064765 34.56069559041587 16.206170226033066 6 33.671182599282865 33.446495923821125 18.27467483593803 14.607646640957986 7 31.36614939005053 30.425748958751768 19.671293262013997 18.536808389183705 8 26.064254587065044 32.63643595022212 22.458238746726355 18.84107071598648 9 25.801440287888433 15.554242315962627 22.352054397719897 36.292262998429045 10 16.21807902795969 28.371912821667518 31.326396530964544 24.083611619408245 11 36.145645590092975 22.14499019550843 21.84335012341503 19.866014090983565 12 23.55196233318199 23.974540647594 30.265592716959212 22.207904302264797 13 28.15374800671678 19.967557779446814 27.05030024117604 24.828393972660372 14 22.343672619856143 19.47084600264582 27.864647610329385 30.320833767168647 15 22.897966583105934 26.66094796243414 25.774918778015444 24.666166676444487 16 25.083930749732037 26.688287115044325 24.49467134874812 23.73311078647552 17 24.266510645584795 26.795299494939883 25.450833275195393 23.487356584279926 18 23.514106180462072 26.28418064494306 26.602619496491357 23.59909367810351 19 25.320890391230748 25.42495152357865 25.538895556764054 23.715262528426553 20 25.320373688671378 25.392281633811965 25.48137346290158 23.80597121461508 21 26.183149865649646 24.71147971425974 24.850533913157456 24.254836506933156 22 25.804877919867653 24.82395566584478 24.75260241173999 24.618564002547576 23 24.847166973055085 24.89341594368514 25.781396179782167 24.478020903477603 24 25.242690735413976 24.909430344085408 25.19062705810095 24.657251862399665 25 24.656033311292827 25.07189592229479 25.773069460366994 24.49900130604539 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3791.0 1 3791.0 2 2245.5 3 700.0 4 700.0 5 700.0 6 774.5 7 849.0 8 849.0 9 849.0 10 1053.0 11 1257.0 12 1257.0 13 1257.0 14 1979.5 15 2702.0 16 2702.0 17 2702.0 18 5334.0 19 7966.0 20 7966.0 21 7966.0 22 14951.5 23 21937.0 24 21937.0 25 21937.0 26 35864.5 27 49792.0 28 49792.0 29 49792.0 30 69675.0 31 89558.0 32 89558.0 33 89558.0 34 115695.0 35 141832.0 36 141832.0 37 141832.0 38 166864.0 39 191896.0 40 191896.0 41 191896.0 42 213349.5 43 234803.0 44 234803.0 45 234803.0 46 250281.5 47 265760.0 48 265760.0 49 265760.0 50 262731.5 51 259703.0 52 259703.0 53 259703.0 54 241860.0 55 224017.0 56 224017.0 57 224017.0 58 196760.0 59 169503.0 60 169503.0 61 169503.0 62 139784.5 63 110066.0 64 110066.0 65 110066.0 66 86143.0 67 62220.0 68 62220.0 69 62220.0 70 47068.0 71 31916.0 72 31916.0 73 31916.0 74 23315.0 75 14714.0 76 14714.0 77 14714.0 78 11099.0 79 7484.0 80 7484.0 81 7484.0 82 5152.5 83 2821.0 84 2821.0 85 2821.0 86 1944.0 87 1067.0 88 1067.0 89 1067.0 90 697.0 91 327.0 92 327.0 93 327.0 94 184.0 95 41.0 96 41.0 97 41.0 98 26.0 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2049840436160493 2 0.21832276867645578 3 0.22306776968608655 4 0.2883378946852298 5 0.22960532663268896 6 0.2512214423432291 7 0.25164322021075186 8 0.24927071970593648 9 0.25775899928983154 10 0.2528031093464394 11 0.25006155320754164 12 0.25180138691107284 13 0.25064149777538536 14 0.24747816376896487 15 0.24758360823584555 16 0.24763633046928588 17 0.2467927747342404 18 0.24726727483520347 19 0.2412569402230045 20 0.28607083864729516 21 0.24594921899919495 22 0.24979794204033987 23 0.25301399828020077 24 0.2540684429490076 25 0.2510632756429081 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1896733.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.07171686208414 #Duplication Level Percentage of deduplicated Percentage of total 1 91.29734253872635 69.45145591866702 2 5.0894301742550665 7.74323382410558 3 1.4432003578174073 3.2936018698543306 4 0.6725745497263012 2.0465560286169167 5 0.3728773685012271 1.418271080045218 6 0.2191011764077268 1.0000441593502887 7 0.14558197776672963 0.7752269695025019 8 0.11220755301801225 0.6828656962378818 9 0.08255969284568222 0.5652411820539636 >10 0.49602014244426124 6.947385179343915 >50 0.04444881719213992 2.359650640242256 >100 0.023623130639546304 3.0455831359556105 >500 9.636859488951284E-4 0.4892344805351747 >1k 6.883471063536597E-5 0.18164983548929833 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNN 3469 0.18289342780454604 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 5.2722233440341894E-5 0.0 0.0 0.0 0.0 11 5.2722233440341894E-5 0.0 0.0 0.0 0.0 12 5.2722233440341894E-5 0.0 0.0 0.0 0.0 13 5.2722233440341894E-5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCCGT 50 0.0015021024 13.297242 6 TTCGCGC 60 4.0898728E-4 12.667726 17 CGCGGTT 135 2.7284841E-11 12.667389 18 GTGCACG 205 0.0 12.509602 11 CTACGCC 115 5.295078E-9 12.396273 4 TGACCGA 285 0.0 12.334363 19 GGTATCA 1265 0.0 12.169919 1 CGCGGGT 95 1.0387776E-6 12.00132 15 TTGACCG 305 0.0 11.836741 18 ACATGGT 775 0.0 11.772459 3 CGGTACT 65 7.997898E-4 11.696995 4 AGACCCG 205 0.0 11.582964 5 CGGTTTG 85 5.3166543E-5 11.177995 14 GCCCCCG 265 0.0 11.113842 18 CTACGGA 60 0.0058589494 11.087777 4 GCATACG 80 3.7603037E-4 10.690936 1 ACGGCCC 240 0.0 10.688676 15 CCTACGC 90 9.4780655E-5 10.559788 3 TAAGGCT 265 0.0 10.400394 4 CCTATAA 265 0.0 10.3995695 1 >>END_MODULE