FastQCFastQC Report
Thu 26 May 2016
SRR1364266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896733
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG21770.11477630219962429No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAT603.893362E-412.74186818
ACTATCC604.1819518E-412.6343338
GGACCGA1251.4697434E-912.1289616
ACGATAC550.003128794712.0597273
TAATACC1052.8135764E-711.7315724
CGATCAA658.227811E-411.65754110
CCTATAA2700.011.5814711
ACGGCCC2650.011.53980415
TATAAGC2900.011.4359483
CGCGGTT1354.311005E-911.32610518
CTTCGCG1104.603571E-711.29392815
ACGTCTT600.00562310311.14913516
CGCGCCA1701.2732926E-1111.14323810
GGTATCA18150.011.0158851
CGGTTTT1053.229481E-610.92160116
GCGAGTA700.001419773110.92160117
AAACGCA700.001523806110.8294285
TCGCGGT1158.171028E-710.80288817
GGGTATG1159.113537E-710.7117181
ATTCGTC908.962189E-510.61822316
Produced by FastQC (version 0.11.5)