##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364266_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1896733 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.40595487082262 31.0 29.0 33.0 26.0 34.0 2 29.469207315948 31.0 29.0 34.0 26.0 34.0 3 29.54372966569359 31.0 29.0 34.0 26.0 34.0 4 33.093325734302084 35.0 32.0 37.0 27.0 37.0 5 32.37363034227801 35.0 32.0 37.0 25.0 37.0 6 32.45710756337344 35.0 32.0 37.0 25.0 37.0 7 32.00438807148924 35.0 32.0 37.0 22.0 37.0 8 31.887023107627694 35.0 32.0 37.0 19.0 37.0 9 32.471393179746435 37.0 32.0 39.0 17.0 39.0 10 32.268798507749906 37.0 31.0 39.0 17.0 39.0 11 32.903233085521265 37.0 32.0 39.0 18.0 39.0 12 32.68543173973353 37.0 32.0 39.0 17.0 39.0 13 32.830642478408926 37.0 32.0 39.0 17.0 39.0 14 33.30081619289589 38.0 32.0 40.0 16.0 41.0 15 33.60301687164192 38.0 32.0 40.0 17.0 41.0 16 33.63490433287131 38.0 32.0 40.0 17.0 41.0 17 33.58805008401288 38.0 32.0 40.0 17.0 41.0 18 33.45809452358345 38.0 32.0 40.0 17.0 41.0 19 33.27978529397654 38.0 32.0 40.0 13.0 41.0 20 32.95483444427866 38.0 32.0 40.0 10.0 41.0 21 32.417441463822264 38.0 31.0 40.0 9.0 41.0 22 32.42519057769333 38.0 31.0 40.0 8.0 41.0 23 32.440873860474824 38.0 31.0 40.0 2.0 41.0 24 32.43735465139268 38.0 32.0 40.0 2.0 41.0 25 32.27279854360102 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6960461739023565 1101 2 0.6659259211533168 1101 3 0.7203391242135275 1101 4 0.7123420035987422 1101 5 0.9825789555969635 1101 6 0.8071600492986946 1101 7 1.2084461864524059 1101 8 1.1839803193123366 1101 9 2.1580248644883397 1101 10 2.3110870967781736 1101 11 1.5062986880735778 1101 12 1.5457472804779684 1101 13 1.24036815119279 1101 14 1.8450191900511541 1101 15 1.4860667515074013 1101 16 1.5220132820091408 1101 17 1.3688505130643165 1101 18 1.5254145502317868 1101 19 1.411244696568744 1101 20 2.2181435572399266 1101 21 2.464974265709195 1101 22 2.2814298891774953 1101 23 2.272436848186942 1101 24 2.075914540519527 1101 25 2.1828816736193417 1102 1 0.12527200296868202 1102 2 0.4172031194531378 1102 3 0.3624562696500142 1102 4 0.23840979945861562 1102 5 0.6582172878871901 1102 6 0.369723497150936 1102 7 0.6541247784504733 1102 8 0.722501043870917 1102 9 1.8767808933587844 1102 10 1.8762452056471943 1102 11 0.9804575659694237 1102 12 0.906400923450164 1102 13 0.8000632498751443 1102 14 1.2760544062679386 1102 15 0.7602587984922877 1102 16 0.6583358048566481 1102 17 0.7314762491064926 1102 18 0.7186478049043075 1102 19 0.6418535337998748 1102 20 1.2523432740861011 1102 21 1.7836155606128443 1102 22 1.7531527305926744 1102 23 1.4987591340002595 1102 24 1.426354756964706 1102 25 1.4824588266001086 1103 1 0.5031053901686917 1103 2 0.6141924475138758 1103 3 0.6235082858480396 1103 4 0.6232635989605058 1103 5 0.9712584103419886 1103 6 0.7206195851627513 1103 7 1.0623155235216757 1103 8 1.0296868585568753 1103 9 1.7840432304120881 1103 10 1.892814753864208 1103 11 1.1414058095988935 1103 12 1.1429440769955406 1103 13 0.9122985902174818 1103 14 1.7209448351100534 1103 15 1.2499407949814412 1103 16 1.0964466371284942 1103 17 1.118848949474689 1103 18 0.7231480488886675 1103 19 0.7264633489421115 1103 20 1.472374865307735 1103 21 2.145668546411059 1103 22 1.839952859872696 1103 23 1.7411183043266902 1103 24 1.5608823462091195 1103 25 1.324603639239136 1104 1 0.312672757882126 1104 2 0.5272122304774776 1104 3 0.23880086883725227 1104 4 0.2449856451207566 1104 5 0.48212143412636266 1104 6 0.36710154681325236 1104 7 0.5906382931365783 1104 8 0.6413261555894287 1104 9 1.3290246638766732 1104 10 1.4867142064715253 1104 11 0.8558925089170515 1104 12 0.5557421819284798 1104 13 0.6322960293160449 1104 14 1.2410695189987848 1104 15 0.3525737217716767 1104 16 0.4865351482844176 1104 17 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2.8728177200707776 2203 23 2.8457927576376605 2203 24 2.619391947955563 2203 25 2.8057982148999088 2204 1 0.717749605571985 2204 2 0.8268960759716855 2204 3 0.8287561705896884 2204 4 0.8665898504625602 2204 5 1.2181669741961585 2204 6 0.9647303094916282 2204 7 1.2569567657946337 2204 8 1.167525516684048 2204 9 2.276765564814923 2204 10 2.567633269033365 2204 11 1.4739213256228254 2204 12 1.7320364050804784 2204 13 1.1312126543627699 2204 14 2.074101618183036 2204 15 1.7307653431275725 2204 16 1.7011636942249808 2204 17 1.5720778928984913 2204 18 1.68647169890167 2204 19 1.6200646150001177 2204 20 2.4327185715624537 2204 21 2.6645123084119717 2204 22 2.5736105534668354 2204 23 2.6742885284263878 2204 24 2.3565267102689234 2204 25 2.541777493035447 2205 1 0.34813822270717054 2205 2 0.4842790923960152 2205 3 0.5127801604489264 2205 4 0.4183149659965153 2205 5 0.7247151720070448 2205 6 0.22414534499927186 2205 7 0.30364955566267327 2205 8 0.8887545889064121 2205 9 1.4197150514342596 2205 10 1.6587166518590521 2205 11 0.9974586469345397 2205 12 1.0647777150837783 2205 13 0.8939288783899784 2205 14 1.1970692093760746 2205 15 0.18778746017365933 2205 16 0.10077210602013054 2205 17 -0.033943062312630445 2205 18 0.512031166297696 2205 19 0.4698638996814708 2205 20 1.1521028754503106 2205 21 1.2432857732141187 2205 22 0.7559551400831452 2205 23 0.2416542472342087 2205 24 0.14850239212483274 2205 25 0.32156468252886583 2206 1 -1.2542294927957585 2206 2 -3.275345170572084 2206 3 -1.0029019688245704 2206 4 -2.1722030523453846 2206 5 -0.5754995625778676 2206 6 -0.7959605641981966 2206 7 -0.6854965825761035 2206 8 0.12626321290284181 2206 9 1.3904720535508375 2206 10 0.8984073183455763 2206 11 0.7005070687306514 2206 12 0.33795498766360055 2206 13 0.3119041797324016 2206 14 0.9248506509705052 2206 15 0.12693080595987993 2206 16 0.47021867871101364 2206 17 0.6499070809719569 2206 18 0.2611947682337501 2206 19 0.4475463513411029 2206 20 1.129177090812945 2206 21 1.595071170392842 2206 22 1.3998883446180912 2206 23 1.282627635997443 2206 24 1.1437166802517567 2206 25 0.774803894047281 2207 1 0.36410541515136075 2207 2 0.4048284750197908 2207 3 0.23647257312374492 2207 4 0.3224671202676035 2207 5 0.6628264475162027 2207 6 0.470292936775067 2207 7 0.5545907263497085 2207 8 0.6372997021466311 2207 9 1.1865244777967163 2207 10 1.6346696232985352 2207 11 0.4609375719387714 2207 12 0.6668406237662197 2207 13 0.683986981396103 2207 14 1.6360185964381344 2207 15 0.9978497456469171 2207 16 0.06588518288737077 2207 17 0.13559826713556333 2207 18 0.6275870006159039 2207 19 0.9437468272790213 2207 20 1.584834502684302 2207 21 1.7145543506343799 2207 22 1.6598159548505578 2207 23 0.9371282599199944 2207 24 1.2240151103539887 2207 25 1.0316895046699912 2208 1 0.14803246807627346 2208 2 0.43975114483758304 2208 3 0.41076366199209957 2208 4 0.21582628963690098 2208 5 0.14397215392410345 2208 6 0.19637174926226209 2208 7 0.6321365667540562 2208 8 0.5974095313747902 2208 9 1.4841722082626276 2208 10 1.5862939313823787 2208 11 0.7630598265148336 2208 12 0.7383802453612418 2208 13 0.5606969085447417 2208 14 1.3089536155252688 2208 15 0.9690828442410648 2208 16 0.847365604313552 2208 17 0.7101038791569536 2208 18 0.2836436214759104 2208 19 0.6894026797454984 2208 20 1.4387271484637196 2208 21 2.020584834825371 2208 22 1.6504378910010047 2208 23 1.6308999101009292 2208 24 1.2293274190071912 2208 25 1.0255598115733875 2209 1 0.24571783634351974 2209 2 0.12127461141959017 2209 3 0.29475729754389235 2209 4 0.12966836109026758 2209 5 0.41701728617948675 2209 6 0.1767840830727465 2209 7 0.32926875901683417 2209 8 0.09813105520341026 2209 9 1.1929515913864392 2209 10 1.3626431275644464 2209 11 0.21734111544555645 2209 12 0.5994596394390399 2209 13 0.3682821221566357 2209 14 0.7706478713693983 2209 15 0.3845654599030226 2209 16 0.27412269701578396 2209 17 -0.013527894440983346 2209 18 0.05689599746850149 2209 19 0.029943360786013784 2209 20 0.8514373859794375 2209 21 1.5371515696295077 2209 22 1.3386623157777962 2209 23 1.2871521728405 2209 24 0.7209657260045574 2209 25 0.6665219887790812 2210 1 -0.2786639717068695 2210 2 0.02263292227079816 2210 3 0.12738583277258897 2210 4 0.1386452632651256 2210 5 -0.15688782689444025 2210 6 -0.009449022400133344 2210 7 0.09955311194422478 2210 8 0.25361397768763894 2210 9 1.282394966134028 2210 10 1.3281520486513259 2210 11 0.47795971532061543 2210 12 0.5023791607171475 2210 13 0.4754033448883206 2210 14 1.019337945329866 2210 15 0.4604102411498232 2210 16 0.6087486802259008 2210 17 0.37379073185100253 2210 18 0.2881712371333691 2210 19 0.4884286839057239 2210 20 1.0728995340801788 2210 21 1.610481311369945 2210 22 1.3698014724375724 2210 23 1.459507261658466 2210 24 1.3615880284097486 2210 25 1.1765330339672815 2211 1 0.5477579318818506 2211 2 0.5394992954781692 2211 3 0.42257865977439835 2211 4 0.5140918938459436 2211 5 0.7747018543049364 2211 6 0.4327475401151375 2211 7 0.8570478586800903 2211 8 0.8663957357398395 2211 9 1.917443598889136 2211 10 1.772216367897471 2211 11 1.071310187210024 2211 12 0.566503253890474 2211 13 0.3184206287778295 2211 14 1.270478400255243 2211 15 0.9323148770324607 2211 16 1.0493925073311559 2211 17 0.7653380378832324 2211 18 0.5921278604982874 2211 19 0.5884615584550517 2211 20 1.3798254971680208 2211 21 2.213207534915611 2211 22 2.058400715180582 2211 23 1.9840880476902143 2211 24 1.7901931384347733 2211 25 1.51473130875155 2212 1 -0.029743549022072102 2212 2 0.041248328931434486 2212 3 -0.1408801791834513 2212 4 -0.05942386640868591 2212 5 -0.32649948056399936 2212 6 -0.3145821997377283 2212 7 0.11944373209666992 2212 8 -0.04345832362166391 2212 9 -10.216816515229755 2212 10 -2.5523788180022997 2212 11 -1.4560156911732314 2212 12 -0.39613652221167683 2212 13 -0.3323386756454738 2212 14 0.36858887467688106 2212 15 0.12419891703270025 2212 16 -0.12492471003858441 2212 17 -0.12463636050586757 2212 18 0.12521991375556496 2212 19 0.2698684138103289 2212 20 0.8288953749420642 2212 21 1.1882775535355776 2212 22 1.024360226357615 2212 23 0.9438164745086226 2212 24 0.7554166622650271 2212 25 0.5519650914215717 2213 1 0.41018391019743206 2213 2 0.3579070647879803 2213 3 0.12722373067825998 2213 4 0.4488346126791285 2213 5 0.39540330037106486 2213 6 0.2283190543983551 2213 7 0.5078502000036593 2213 8 0.6455710458403452 2213 9 1.4545622021360032 2213 10 -8.996610113444692 2213 11 -2.6170469237813343 2213 12 -0.6011876097519 2213 13 0.220269460242271 2213 14 0.7592733786489134 2213 15 0.45931756999527096 2213 16 0.870873930681455 2213 17 0.5312474327900247 2213 18 0.33033020151258086 2213 19 0.8908392211674538 2213 20 1.8227276111013069 2213 21 1.7086018378650323 2213 22 1.9107946725215115 2213 23 1.366365348634794 2213 24 1.426587104280614 2213 25 1.1895499609402904 2214 1 -1.9470292809131102 2214 2 -2.269922261115454 2214 3 -2.1820377570391543 2214 4 -2.063034620834017 2214 5 -2.0048607039892765 2214 6 -2.0909111839954484 2214 7 -10.236337529809017 2214 8 -3.760792296906857 2214 9 -2.869169771597363 2214 10 -2.2612609921333977 2214 11 -2.9173404993285494 2214 12 -11.963005979137062 2214 13 -5.603477973712366 2214 14 -13.421161458345544 2214 15 -7.087703428077262 2214 16 -5.016163151643099 2214 17 -4.198189418811804 2214 18 -3.6090567360621364 2214 19 -3.903412640454011 2214 20 -4.028684342798002 2214 21 -12.791940534806692 2214 22 -15.19442576634076 2214 23 -9.86029161463481 2214 24 -8.338992652611626 2214 25 -15.81852955069662 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57988.0 3 4.0 4 930.0 5 9231.0 6 11198.0 7 9368.0 8 8312.0 9 6542.0 10 5803.0 11 5237.0 12 5155.0 13 5148.0 14 4873.0 15 5096.0 16 5425.0 17 5937.0 18 6522.0 19 7768.0 20 9798.0 21 12871.0 22 16342.0 23 20245.0 24 23927.0 25 28403.0 26 32383.0 27 36545.0 28 40738.0 29 48433.0 30 56807.0 31 68481.0 32 85075.0 33 102400.0 34 123828.0 35 146802.0 36 179402.0 37 236170.0 38 467546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.553633582955385 17.759835460394473 12.423689484231621 26.26284147241852 2 18.073780821542204 20.801304731603043 35.35488341044102 25.770031036413737 3 20.520028702437862 23.121753906647484 28.092906480350376 28.265310910564278 4 14.300905820692739 16.192421389831885 33.92738988566129 35.57928290381408 5 15.05351002344128 36.51620168992341 32.69731854096741 15.732969745667896 6 34.11268489301183 34.08226371523247 17.4839524332988 14.3210989584569 7 31.184936919951685 30.523320031401628 19.64483895067108 18.646904097975607 8 28.146107180568553 33.54743567699625 18.978173797375288 19.32828334505991 9 27.383598681449396 15.859514886419475 18.54427963733531 38.21260679479582 10 16.240480674530282 28.623037874841568 30.784407181436073 24.352074269192077 11 36.78858155770355 21.88214911441272 21.354429465410345 19.97483986247338 12 24.924549988927673 24.51909185814765 27.873111113454463 22.683247039470217 13 29.423273823143916 19.958549190016797 25.326645247075717 25.291531739763567 14 23.458669925650046 19.68939835694093 25.701189325071994 31.150742392337033 15 25.38391093297988 27.440873208560635 22.010577156778854 25.16463870168063 16 25.414320074317693 26.979177760367623 23.598028598814164 24.00847356650052 17 24.887896750600653 27.039646457590045 24.558849658536534 23.51360713327277 18 24.01724876048918 26.258438269092473 26.101957277736886 23.622355692681456 19 25.378065928434385 25.420402774724216 25.420033711306996 23.7814975855344 20 25.250626999683508 25.385947228302353 25.51658656850985 23.846839203504285 21 25.90880999938833 24.44601346947643 25.050146696223862 24.59502983491138 22 25.82904541737385 24.842231582788017 24.61419301500731 24.714529984830822 23 25.075543515226546 24.790030945028153 25.362374241335367 24.772051298409934 24 25.499288237465073 24.84446670533031 24.844203089576634 24.812041967627984 25 24.997350691008325 25.040108692844125 25.412382738420675 24.55015787772688 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 34.0 1 34.0 2 57.0 3 80.0 4 80.0 5 80.0 6 171.0 7 262.0 8 262.0 9 262.0 10 389.0 11 516.0 12 516.0 13 516.0 14 788.5 15 1061.0 16 1061.0 17 1061.0 18 2906.0 19 4751.0 20 4751.0 21 4751.0 22 10430.0 23 16109.0 24 16109.0 25 16109.0 26 29307.0 27 42505.0 28 42505.0 29 42505.0 30 59753.0 31 77001.0 32 77001.0 33 77001.0 34 102964.0 35 128927.0 36 128927.0 37 128927.0 38 152945.5 39 176964.0 40 176964.0 41 176964.0 42 199020.5 43 221077.0 44 221077.0 45 221077.0 46 243245.5 47 265414.0 48 265414.0 49 265414.0 50 268628.0 51 271842.0 52 271842.0 53 271842.0 54 254548.0 55 237254.0 56 237254.0 57 237254.0 58 211469.5 59 185685.0 60 185685.0 61 185685.0 62 155678.5 63 125672.0 64 125672.0 65 125672.0 66 98705.5 67 71739.0 68 71739.0 69 71739.0 70 54785.0 71 37831.0 72 37831.0 73 37831.0 74 27314.5 75 16798.0 76 16798.0 77 16798.0 78 13164.0 79 9530.0 80 9530.0 81 9530.0 82 6658.0 83 3786.0 84 3786.0 85 3786.0 86 2570.0 87 1354.0 88 1354.0 89 1354.0 90 915.5 91 477.0 92 477.0 93 477.0 94 268.0 95 59.0 96 59.0 97 59.0 98 32.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02810095042370223 2 0.013233280593525815 3 0.0015816670032102569 4 0.0 5 0.00416505644178701 6 0.0014762225363295731 7 8.435557350454703E-4 8 0.03980528624745813 9 0.005535834511235899 10 0.0034269451736222228 11 0.004323223142108036 12 0.005957612378758634 13 0.0014235003028892311 14 0.0014235003028892311 15 2.1088893376136758E-4 16 5.799445678437608E-4 17 0.0023197782713750433 18 8.962779684858121E-4 19 0.002108889337613676 20 0.8829919656588461 21 0.015658503331781544 22 0.0065375569466023945 23 0.007592001615409232 24 0.0017398337035312824 25 6.326668012841027E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1896733.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.19926852537796 #Duplication Level Percentage of deduplicated Percentage of total 1 76.73264051684822 39.28655066483409 2 12.395483580784477 12.692793847089959 3 4.318631047190719 6.6333225194145555 4 1.9751499289174252 4.045049263540978 5 1.1162468109017065 2.8575510105976636 6 0.7338642609068832 2.254398801320973 7 0.476518416179796 1.7078176043094033 8 0.34408870150408544 1.4093671859885037 9 0.24930246504930886 1.1487693466888396 >10 1.446756086788582 13.742221279022154 >50 0.12194582657375434 4.394491318651697 >100 0.08474828099058464 7.9126950330498795 >500 0.003656247218526249 1.2482198055551186 >1k 9.678301460804776E-4 0.6667523199361514 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTATCAACGCAGAGTACATGGG 2177 0.11477630219962429 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.2722233440341894E-5 0.0 2 0.0 0.0 0.0 5.2722233440341894E-5 0.0 3 0.0 0.0 0.0 1.0544446688068379E-4 0.0 4 0.0 0.0 0.0 1.0544446688068379E-4 0.0 5 0.0 0.0 0.0 1.0544446688068379E-4 0.0 6 0.0 0.0 0.0 1.0544446688068379E-4 0.0 7 0.0 0.0 0.0 1.0544446688068379E-4 0.0 8 0.0 0.0 0.0 1.0544446688068379E-4 0.0 9 0.0 0.0 0.0 1.0544446688068379E-4 0.0 10 5.2722233440341894E-5 0.0 0.0 1.0544446688068379E-4 0.0 11 5.2722233440341894E-5 0.0 0.0 1.0544446688068379E-4 0.0 12 5.2722233440341894E-5 0.0 0.0 1.0544446688068379E-4 0.0 13 1.0544446688068379E-4 0.0 0.0 1.0544446688068379E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTAT 60 3.893362E-4 12.741868 18 ACTATCC 60 4.1819518E-4 12.634333 8 GGACCGA 125 1.4697434E-9 12.128961 6 ACGATAC 55 0.0031287947 12.059727 3 TAATACC 105 2.8135764E-7 11.731572 4 CGATCAA 65 8.227811E-4 11.657541 10 CCTATAA 270 0.0 11.581471 1 ACGGCCC 265 0.0 11.539804 15 TATAAGC 290 0.0 11.435948 3 CGCGGTT 135 4.311005E-9 11.326105 18 CTTCGCG 110 4.603571E-7 11.293928 15 ACGTCTT 60 0.005623103 11.149135 16 CGCGCCA 170 1.2732926E-11 11.143238 10 GGTATCA 1815 0.0 11.015885 1 CGGTTTT 105 3.229481E-6 10.921601 16 GCGAGTA 70 0.0014197731 10.921601 17 AAACGCA 70 0.0015238061 10.829428 5 TCGCGGT 115 8.171028E-7 10.802888 17 GGGTATG 115 9.113537E-7 10.711718 1 ATTCGTC 90 8.962189E-5 10.618223 16 >>END_MODULE