FastQCFastQC Report
Thu 26 May 2016
SRR1364265_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364265_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4265846
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT145930.3420892362265305No Hit
TATCAACGCAGAGTACTTTTTTTTT95350.22351955508942423No Hit
GGTATCAACGCAGAGTACTTTTTTT83790.19642059277339124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA83430.1955766804521307No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN81090.19009125036393718No Hit
GTACTTTTTTTTTTTTTTTTTTTTT47210.11066972412975058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54050.014.204971
ACATGGT21950.011.6460373
GTATCAA142100.011.3879411
CTCAACG3900.011.2086594
CGCTCGG855.3207892E-511.17788919
CATGGTA17350.011.1187754
GTGGTAT13850.011.1144991
CGCATAT4500.010.764118
TCGACCG1158.812567E-710.7406218
TTGCGCG7000.010.72291518
CGGTTTG2150.010.6068714
TTTGCGC7250.010.35340217
GTTATCA8950.010.2984861
GTACTAG2600.010.23315051
ACTCGAG750.002654155710.13104510
ATTTGCG7200.010.02952216
TGCGCGC7500.010.007936519
TCAACTC31450.09.7296144
AACGCAT19650.09.7153636
TGGTATC15700.09.6835542
Produced by FastQC (version 0.11.5)