FastQCFastQC Report
Thu 26 May 2016
SRR1364265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4265846
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT530011.2424499149758337No Hit
TATCAACGCAGAGTACTTTTTTTTT405610.9508313239624684No Hit
GGTATCAACGCAGAGTACTTTTTTT358930.8414040263056847No Hit
ACGCAGAGTACTTTTTTTTTTTTTT181940.4265039103615086No Hit
GTACTTTTTTTTTTTTTTTTTTTTT129170.3028004292700674No Hit
GAGTACTTTTTTTTTTTTTTTTTTT109800.25739325798446544No Hit
GTGGTATCAACGCAGAGTACATGGG88140.20661786665528947No Hit
ATCAACGCAGAGTACTTTTTTTTTT65420.1533576223801797No Hit
GCAGAGTACTTTTTTTTTTTTTTTT63170.1480831703723013No Hit
GGTATCAACGCAGAGTACATGGGAT55370.12979840341165622No Hit
GTGGTATCAACGCAGAGTACTTTTT53700.12588358792136425No Hit
GGTATCAACGCAGAGTACATGGGAG43420.10178520274759098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT25250.014.7762551
GGTATCA101200.014.2043321
TGGTATC26900.013.4834642
CGGTTTG2350.012.21760714
TCTAACG550.00314629612.0513123
CTGGTCG2650.011.7867469
TCGACGG751.9377378E-411.4845514
GGTCGGT2450.011.203341511
ATCACGC1701.2732926E-1111.1398693
ACCGTTC700.0015337210.8215868
TGGTCGG2800.010.81701910
CTCGACG909.8895965E-510.516313
TAATACC4100.010.3926824
TATCACG1701.6498234E-910.0271762
GTATCAA216550.010.0008641
TTAGGCA5550.09.8954144
AACGCCG3650.09.8580215
TAGGCAT5350.09.734375
GACATAT7800.09.7123631
TCACGCA2253.6379788E-129.6793094
Produced by FastQC (version 0.11.5)