##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364265_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4265846 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.845472386954427 31.0 28.0 33.0 24.0 34.0 2 28.88684425082387 31.0 28.0 34.0 23.0 34.0 3 28.945326905847047 31.0 28.0 34.0 24.0 34.0 4 32.40175735364099 35.0 32.0 37.0 26.0 37.0 5 31.74304276338152 35.0 32.0 37.0 20.0 37.0 6 31.754395962723454 35.0 32.0 37.0 19.0 37.0 7 31.322950945720965 35.0 32.0 37.0 17.0 37.0 8 31.118794724422777 35.0 32.0 37.0 15.0 37.0 9 31.434620002691144 37.0 30.0 39.0 10.0 39.0 10 31.456347463082352 37.0 30.0 39.0 10.0 39.0 11 31.99330636877187 37.0 31.0 39.0 11.0 39.0 12 31.852956482723474 37.0 31.0 39.0 10.0 39.0 13 31.905494478703638 37.0 31.0 39.0 10.0 39.0 14 32.366967537037205 38.0 31.0 40.0 10.0 41.0 15 32.595762247394774 38.0 31.0 40.0 10.0 41.0 16 32.61296540006367 38.0 31.0 40.0 10.0 41.0 17 32.67720963204016 38.0 31.0 40.0 10.0 41.0 18 32.59202066835043 38.0 31.0 40.0 8.0 41.0 19 32.483277642934134 38.0 31.0 40.0 2.0 41.0 20 32.17808847295472 38.0 31.0 40.0 2.0 41.0 21 31.8336332347675 38.0 30.0 40.0 2.0 41.0 22 31.85415601032011 38.0 30.0 40.0 2.0 41.0 23 31.771290618554914 38.0 30.0 40.0 2.0 41.0 24 31.763585464641714 38.0 31.0 40.0 2.0 41.0 25 31.63572384000735 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0783742956936813 1101 2 1.059249933327017 1101 3 1.0448796591894691 1101 4 1.0644776409969694 1101 5 1.466851229256541 1101 6 1.3638847856029308 1101 7 1.591392089591178 1101 8 1.6621967422713482 1101 9 2.550250629630739 1101 10 2.696530675926457 1101 11 1.8406969511188613 1101 12 1.94867152093191 1101 13 1.639364583457592 1101 14 2.239055739484602 1101 15 2.0244415253698733 1101 16 1.892375585782382 1101 17 1.793022098233621 1101 18 1.8946465948646534 1101 19 1.9267940295896437 1101 20 2.606319046635221 1101 21 2.877011960694311 1101 22 2.735868713637192 1101 23 2.686872417744297 1101 24 2.4959976214768886 1101 25 2.6884570035176125 1102 1 0.5176524259293309 1102 2 0.6799329111312282 1102 3 0.670043442011373 1102 4 0.6514737908322772 1102 5 1.060847704973611 1102 6 0.7178775761110394 1102 7 0.9996491601785387 1102 8 1.066804827528447 1102 9 2.0819077631541134 1102 10 2.3169779964593467 1102 11 1.4489599222302836 1102 12 1.4186128969850387 1102 13 1.1084587484168367 1102 14 1.6726498582332603 1102 15 1.2276328069324265 1102 16 1.137881742439518 1102 17 1.2568235396635643 1102 18 1.1667642459608771 1102 19 1.2046203504279873 1102 20 1.8179236812059187 1102 21 2.3527608577247463 1102 22 2.309461712508277 1102 23 2.141239843522989 1102 24 2.1432590123796658 1102 25 2.110398561939995 1103 1 0.8411504970089716 1103 2 0.822253121433949 1103 3 0.9401098025592738 1103 4 1.008532052446867 1103 5 1.263274255933581 1103 6 1.1735093797589862 1103 7 1.375372881082523 1103 8 1.2804202005411867 1103 9 2.1091298812946135 1103 10 2.2443526210245714 1103 11 1.5786296607487564 1103 12 1.5803000320580836 1103 13 1.3563201542152754 1103 14 2.111357139505518 1103 15 1.7208842517191627 1103 16 1.4799322677026154 1103 17 1.5759987595001874 1103 18 1.2397922267711579 1103 19 1.290244911655499 1103 20 1.8457694119893162 1103 21 2.426259246974098 1103 22 2.109902199828422 1103 23 2.0757409430955818 1103 24 2.070910905395124 1103 25 1.93199615045053 1104 1 0.5752889720515242 1104 2 0.781231186379383 1104 3 0.592010355416889 1104 4 0.5529806135540056 1104 5 0.8639827889853038 1104 6 0.7367498526599299 1104 7 1.111135577067774 1104 8 1.214748181194416 1104 9 1.8566634649518434 1104 10 2.063749644968116 1104 11 1.4963615977359375 1104 12 1.1955283797601126 1104 13 1.257444122381969 1104 14 1.8310586200954297 1104 15 0.9306494625704289 1104 16 1.1243822672815043 1104 17 0.7417282804510776 1104 18 1.295027321129318 1104 19 1.5246420656577584 1104 20 2.041586828036415 1104 21 2.6009937054606596 1104 22 2.6048084073706796 1104 23 2.362260457828249 1104 24 2.3038121233036932 1104 25 2.161972083252877 1105 1 0.10347004829848316 1105 2 0.5535384049183847 1105 3 0.506682696758535 1105 4 0.7415265545374794 1105 5 0.8848221154836651 1105 6 0.6689394634673214 1105 7 0.6187531070011687 1105 8 0.9494664076515349 1105 9 1.8143195696714045 1105 10 1.927417787059543 1105 11 1.1388902782496864 1105 12 1.5025700562046467 1105 13 0.9905174745521208 1105 14 1.2165599212753584 1105 15 1.3512236033403298 1105 16 0.689479819290284 1105 17 1.1337543922338682 1105 18 0.8393404185105169 1105 19 0.9535604681898064 1105 20 1.6023539346278426 1105 21 2.258732504081749 1105 22 1.6932783725116813 1105 23 1.872622492485867 1105 24 1.7069564992574016 1105 25 1.3213825897875822 1106 1 0.319651320127484 1106 2 0.36007352924027103 1106 3 0.03500909163800969 1106 4 0.28783908464059493 1106 5 0.46878464788046514 1106 6 0.16428525376787917 1106 7 0.4676088381157868 1106 8 0.5027111353896494 1106 9 1.1999016923182566 1106 10 1.351533421002305 1106 11 0.760784692342181 1106 12 0.7873040270408254 1106 13 0.742619672920874 1106 14 1.3116638910605296 1106 15 0.24839851657531398 1106 16 0.3279163718207272 1106 17 0.05573118364439722 1106 18 -0.3322010135616509 1106 19 0.26516801647939303 1106 20 0.8105932320748721 1106 21 -5.047976024026173 1106 22 -1.2209092932732908 1106 23 -0.2590693030022386 1106 24 0.3588869166473998 1106 25 0.6817787592827855 1107 1 0.5317787651267558 1107 2 0.2945890010129979 1107 3 0.44392797943103446 1107 4 0.4287637022411417 1107 5 0.7991817772801539 1107 6 0.4380556776908229 1107 7 0.8019035274080757 1107 8 0.7966604688562313 1107 9 1.805176352062574 1107 10 2.069668232167839 1107 11 0.3995465553874027 1107 12 0.7004964717359243 1107 13 0.49797445892445324 1107 14 1.3720239113870072 1107 15 0.961760837007418 1107 16 1.1837948041465722 1107 17 1.0275599647728129 1107 18 1.0255951722687087 1107 19 1.2482873107196326 1107 20 1.4686308051041053 1107 21 2.1274253831369734 1107 22 1.8492249535057077 1107 23 1.8454900198590423 1107 24 1.349555111284694 1107 25 1.4950028231243166 1108 1 -0.12978130445018365 1108 2 -0.019000559276719287 1108 3 0.11132384596374223 1108 4 -0.9169355460931108 1108 5 0.13809385763049775 1108 6 0.24436241480682597 1108 7 0.4057329001600465 1108 8 0.6110214771442415 1108 9 1.417080425714552 1108 10 1.1016642191490398 1108 11 0.6729073402092887 1108 12 0.7065025902605555 1108 13 0.3673652483545311 1108 14 0.9243115851302086 1108 15 0.7807938804311547 1108 16 0.5224476194917358 1108 17 0.4300316040420924 1108 18 0.3826222873227536 1108 19 0.4967541397165931 1108 20 1.216975575421742 1108 21 1.5829459718811805 1108 22 1.516484917193953 1108 23 1.1494399518607636 1108 24 1.2069457272580308 1108 25 0.8677016694240116 1109 1 0.17206520926861657 1109 2 0.2746201502907901 1109 3 -0.15106752584629035 1109 4 0.3226177347131767 1109 5 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0.38935540684884273 1110 18 0.7741238530191055 1110 19 0.5985341129562727 1110 20 1.3109647602125136 1110 21 1.3259273785923682 1110 22 1.644083270335873 1110 23 1.4406049858206984 1110 24 1.171551218380948 1110 25 1.5023197924440836 1111 1 -0.15720383790507597 1111 2 -0.07472622496441517 1111 3 -0.2980651466873674 1111 4 -0.05682296674270049 1111 5 0.08652982570220757 1111 6 -0.36112196650226736 1111 7 0.1320792547679197 1111 8 0.01046079024794011 1111 9 1.014564763557921 1111 10 1.002985331509965 1111 11 0.08829444554956112 1111 12 0.2673492439825864 1111 13 0.004206960149534922 1111 14 0.0885927308134633 1111 15 0.21016519319151428 1111 16 0.17580614831501862 1111 17 -0.4103115954351111 1111 18 -0.05131559044568945 1111 19 0.23372504187216947 1111 20 0.48178300634637594 1111 21 1.1677433868828473 1111 22 1.2064193273173736 1111 23 0.9402020339335522 1111 24 0.9429686901145331 1111 25 0.8795284725291559 1112 1 0.4613425209161548 1112 2 0.4610766489200593 1112 3 0.43736273108600443 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1.609180757654542 2204 6 1.5039717078830783 2204 7 1.8276730747073238 2204 8 1.724303101341107 2204 9 2.742890357574062 2204 10 3.0012899018855954 2204 11 1.9774073195734339 2204 12 2.2978105281444385 2204 13 1.8780671996574085 2204 14 2.6397021242859005 2204 15 2.3748812506552 2204 16 2.3573774460279395 2204 17 2.3123096980116316 2204 18 2.3163461567896277 2204 19 2.365872941459955 2204 20 3.0332699744623923 2204 21 3.2317164288706692 2204 22 3.267607370717055 2204 23 3.2779776512488326 2204 24 3.0550957384624553 2204 25 3.287676640755045 2205 1 0.822557437219551 2205 2 0.9232082289553709 2205 3 0.9968280752253555 2205 4 0.8883324361328064 2205 5 1.2980953390412822 2205 6 0.868152305175304 2205 7 0.8762893622092243 2205 8 1.4607998709103498 2205 9 2.0237564604052025 2205 10 2.1657736004439947 2205 11 1.5047227735183846 2205 12 1.6708815930669232 2205 13 1.5143777009891508 2205 14 1.5873406760971456 2205 15 0.8280287583488999 2205 16 0.7531083120845139 2205 17 0.7822758108119032 2205 18 1.2605442573332013 2205 19 1.270374296969294 2205 20 1.7763273252551386 2205 21 1.8965453466498587 2205 22 1.3713654891324758 2205 23 0.682859036087752 2205 24 0.8620473712678951 2205 25 1.1233854793846447 2206 1 -0.9894485811503877 2206 2 -2.929663212803945 2206 3 -0.7987050939691756 2206 4 -1.8055294561866475 2206 5 -0.24650105023810198 2206 6 -0.5931926100229497 2206 7 -0.30420944433878816 2206 8 0.3606393879181624 2206 9 1.4934990999915883 2206 10 1.1748849456010646 2206 11 0.8185758490670416 2206 12 0.5800306462156861 2206 13 0.5198728378477497 2206 14 0.9808801353494765 2206 15 0.41431457985060405 2206 16 0.845989257367961 2206 17 0.8704770951992273 2206 18 0.18951118762274177 2206 19 0.6533468587947411 2206 20 1.2404173822485305 2206 21 1.689721064543015 2206 22 1.6175658280840999 2206 23 1.2897907245120521 2206 24 1.160150322253294 2206 25 1.1197174235888738 2207 1 0.5671663078845945 2207 2 0.6866390675557454 2207 3 0.4893500950870937 2207 4 0.6461418080276076 2207 5 0.9510282926766465 2207 6 0.87143703912243 2207 7 0.7767771328517483 2207 8 1.0303747273353885 2207 9 1.5196302432551008 2207 10 1.9807315595984782 2207 11 0.8860396609722265 2207 12 1.0701298816942426 2207 13 1.0591996520450664 2207 14 2.058897575490949 2207 15 1.3799081256196217 2207 16 0.5959400469817737 2207 17 0.7536564535888672 2207 18 1.2504117672598554 2207 19 1.406518630172478 2207 20 1.9216310947392046 2207 21 2.1274169507227754 2207 22 2.054843874376669 2207 23 1.2299003191364264 2207 24 1.644040679024478 2207 25 1.3906293331680573 2208 1 0.39167214620767865 2208 2 0.695090286913377 2208 3 0.6811793095765886 2208 4 0.640702725211959 2208 5 0.7717700165134289 2208 6 0.7226314102873239 2208 7 1.1425582284780909 2208 8 1.1039244108703343 2208 9 1.9874511759986504 2208 10 2.038772343062991 2208 11 1.326943169156504 2208 12 1.3890411960366578 2208 13 1.114885762889621 2208 14 1.9251925835693022 2208 15 1.586802730414007 2208 16 1.4422148606847358 2208 17 1.287701358009599 2208 18 1.00422084531359 2208 19 1.298177375405281 2208 20 2.0696571337748253 2208 21 2.5827349638521397 2208 22 2.2306720338820547 2208 23 2.311551019210114 2208 24 2.134278726410642 2208 25 1.8661505031623307 2209 1 0.4664339018304098 2209 2 0.35105340959286124 2209 3 0.4129152555316935 2209 4 0.35495691095328397 2209 5 0.7677694890317213 2209 6 0.4063583249497569 2209 7 0.6221992356628903 2209 8 0.586069933548842 2209 9 1.3836383501955183 2209 10 1.4532768384098311 2209 11 0.46044478991119675 2209 12 0.8828546415086933 2209 13 0.5881783842813846 2209 14 1.047880493584831 2209 15 0.679346175156482 2209 16 0.5253697568474607 2209 17 0.17406107183856534 2209 18 0.293618890395571 2209 19 0.24739969093397463 2209 20 1.146297020366255 2209 21 1.7300285980820362 2209 22 1.7230283264060695 2209 23 1.5334916329509554 2209 24 0.9939603356449922 2209 25 0.9532480503418768 2210 1 0.010774776778198714 2210 2 0.2384777945210672 2210 3 0.3717716951231047 2210 4 0.4697945359551454 2210 5 0.1695790014783789 2210 6 0.39145227452102915 2210 7 0.6478915017012703 2210 8 0.5360018177183825 2210 9 1.5083943042812322 2210 10 1.5344353956067813 2210 11 0.7258949983835556 2210 12 0.7850626553608677 2210 13 0.6546407388831241 2210 14 1.2085841486122426 2210 15 0.7395212120315762 2210 16 0.8480756004904322 2210 17 0.6580415063314007 2210 18 0.537818663315079 2210 19 0.72636527973998 2210 20 1.3792822718403315 2210 21 1.790318986573773 2210 22 1.5887102639386335 2210 23 1.5861109001920113 2210 24 1.589420398524794 2210 25 1.5645951529831237 2211 1 0.16440181563338285 2211 2 0.10653877413279034 2211 3 -0.04793802504735467 2211 4 0.0316670174978384 2211 5 0.2247232901715499 2211 6 -0.15950885687497163 2211 7 0.24394197391469774 2211 8 0.28410673003943643 2211 9 1.1696800436900787 2211 10 1.182118567025956 2211 11 0.4641420935800973 2211 12 -0.10011521250301314 2211 13 -0.25853894908134833 2211 14 0.40020336871224416 2211 15 0.1882813387208131 2211 16 0.208549597504053 2211 17 -0.0042587238183244835 2211 18 -0.13825019715745412 2211 19 -0.0341905781080456 2211 20 0.5115364236717355 2211 21 1.3644754462534152 2211 22 1.2500307856196606 2211 23 1.0666170985052688 2211 24 0.9571261433109228 2211 25 0.6802604080211339 2212 1 0.18316560261177628 2212 2 0.18574843457339085 2212 3 -0.010820948641327988 2212 4 0.158375578841067 2212 5 0.0726230531626797 2212 6 0.16305378663927783 2212 7 0.4830340179269399 2212 8 0.333602857473295 2212 9 -9.279237402052953 2212 10 -1.94491038183072 2212 11 -0.7699529728450045 2212 12 0.07814105755582901 2212 13 0.06423451633279598 2212 14 0.5113542004591132 2212 15 0.26671937321408734 2212 16 0.10647468296002671 2212 17 0.231306237410692 2212 18 0.44111572899428353 2212 19 0.6915151565531517 2212 20 1.1799787068331256 2212 21 1.4485609695421076 2212 22 1.258054372694108 2212 23 1.1266750522856057 2212 24 1.0789754940335214 2212 25 1.070147637860984 2213 1 0.3831193223839193 2213 2 0.3383817710915622 2213 3 0.16932397013504286 2213 4 0.4908091492100084 2213 5 0.45181115098806046 2213 6 0.19789038111279567 2213 7 0.5686538942289268 2213 8 0.8529375465829787 2213 9 1.3999399586844774 2213 10 -8.625271215561845 2213 11 -2.6464814357951987 2213 12 -0.7634515116284675 2213 13 0.1019853760976055 2213 14 0.6199880956061925 2213 15 0.44201932183749904 2213 16 0.8116354680363784 2213 17 0.5144352219355781 2213 18 0.379941217339379 2213 19 0.9252707121045347 2213 20 1.4331328672012944 2213 21 1.3228290596112586 2213 22 1.6214268056713088 2213 23 1.266406754607722 2213 24 1.31572265194324 2213 25 1.0168284698501502 2214 1 -2.4234613387019266 2214 2 -2.7186856582991368 2214 3 -2.6967842204391808 2214 4 -2.7191232218341383 2214 5 -2.8231981708055045 2214 6 -2.9397468855797797 2214 7 -10.575882812630862 2214 8 -4.469822334518202 2214 9 -3.9140846797349624 2214 10 -3.436954902160519 2214 11 -4.0735147684283035 2214 12 -12.118602327744927 2214 13 -6.131778947954199 2214 14 -13.618128586990672 2214 15 -7.939062491999721 2214 16 -6.176786798708715 2214 17 -5.22281722536346 2214 18 -4.747871732338691 2214 19 -5.10912670615253 2214 20 -5.396026884707702 2214 21 -13.35691514249573 2214 22 -15.360075014047826 2214 23 -10.733504950939139 2214 24 -9.697573376710118 2214 25 -16.270178065908777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 185958.0 3 4.0 4 2914.0 5 26718.0 6 34454.0 7 28173.0 8 23643.0 9 17869.0 10 15115.0 11 13532.0 12 12550.0 13 11949.0 14 11701.0 15 12056.0 16 12361.0 17 13465.0 18 15544.0 19 19819.0 20 25901.0 21 33649.0 22 41945.0 23 49029.0 24 57191.0 25 65125.0 26 73268.0 27 81768.0 28 90096.0 29 104067.0 30 122207.0 31 148322.0 32 182492.0 33 221510.0 34 267449.0 35 320134.0 36 394051.0 37 530366.0 38 999451.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.334192496067054 21.45833963518881 14.719422635139335 22.4880452336048 2 15.46017240801276 22.946362863326243 38.663255299041325 22.930209429619676 3 17.85580915270536 28.430131251586754 31.13048337969847 22.583576216009416 4 12.0515133457701 19.012172497553827 37.76749090332844 31.16882325334764 5 11.822799831305806 40.37328395972813 33.931022454191066 13.872893754775001 6 28.08205000294201 39.64863241008873 18.192601650011287 14.07671593695797 7 25.48373522996192 35.3227226215858 22.1670653790659 17.026476769386388 8 25.90798083223232 34.81704368560696 20.422551065347605 18.85242441681312 9 26.52551787935954 15.9365925257706 20.946137452096615 36.591752142773245 10 15.079948164033452 31.626503482142944 31.527881054835667 21.765667298987943 11 32.00148536194093 25.22048927390979 23.552153059796368 19.22587230435291 12 22.67240684094741 29.394684181012416 29.574939844448906 18.357969133591272 13 27.739690301739543 23.541146781409665 27.323727397078834 21.39543551977195 14 22.000282715200537 23.261834831028438 29.3492369619884 25.388645491782626 15 23.700060457125254 30.0413541739679 25.555126044549798 20.70345932435705 16 21.303015498984017 30.028003942024856 27.27043847056187 21.398542088429256 17 20.057152836953833 29.65796207236468 28.59578198935476 21.68910310132673 18 19.613564870283813 27.39027707027945 32.079418389882534 20.916739669554197 19 21.214998928688136 27.466818635106492 32.27532105364577 19.042861382559607 20 21.970083248529775 26.27265191333657 31.955047358431393 19.802217479702257 21 22.914603001406476 25.759114259435577 31.064268661258488 20.26201407789946 22 22.244487991371052 26.268408429120033 31.153278018473664 20.33382556103526 23 21.458824809131762 26.363693692785535 31.456178077891728 20.721303420190974 24 21.156932419209724 26.747328401391723 31.665329675713664 20.430409503684892 25 21.001028169403686 26.287567683130664 32.181913314020186 20.529490833445465 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3272.0 1 3272.0 2 4494.5 3 5717.0 4 5717.0 5 5717.0 6 11958.0 7 18199.0 8 18199.0 9 18199.0 10 21042.0 11 23885.0 12 23885.0 13 23885.0 14 27559.5 15 31234.0 16 31234.0 17 31234.0 18 55928.0 19 80622.0 20 80622.0 21 80622.0 22 121447.5 23 162273.0 24 162273.0 25 162273.0 26 229220.0 27 296167.0 28 296167.0 29 296167.0 30 341154.0 31 386141.0 32 386141.0 33 386141.0 34 411259.5 35 436378.0 36 436378.0 37 436378.0 38 447629.0 39 458880.0 40 458880.0 41 458880.0 42 475328.5 43 491777.0 44 491777.0 45 491777.0 46 503702.0 47 515627.0 48 515627.0 49 515627.0 50 487942.5 51 460258.0 52 460258.0 53 460258.0 54 401644.5 55 343031.0 56 343031.0 57 343031.0 58 292836.0 59 242641.0 60 242641.0 61 242641.0 62 197684.5 63 152728.0 64 152728.0 65 152728.0 66 117031.0 67 81334.0 68 81334.0 69 81334.0 70 60938.5 71 40543.0 72 40543.0 73 40543.0 74 29124.0 75 17705.0 76 17705.0 77 17705.0 78 13769.0 79 9833.0 80 9833.0 81 9833.0 82 7203.5 83 4574.0 84 4574.0 85 4574.0 86 3303.0 87 2032.0 88 2032.0 89 2032.0 90 1465.0 91 898.0 92 898.0 93 898.0 94 494.5 95 91.0 96 91.0 97 91.0 98 48.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028810228967477966 2 0.011697562453028075 3 0.0016174986157493731 4 0.0 5 0.0032584392404226502 6 0.0013361945086625257 7 7.267022766410227E-4 8 0.03760098231394195 9 0.005368220043574006 10 0.0019222447317601243 11 0.005133799954334967 12 0.005579198123889142 13 0.0018988027228362206 14 0.0016174986157493731 15 3.0474611601075146E-4 16 4.2195616063027126E-4 17 0.00213322281207526 18 7.735862944888306E-4 19 0.00112521642834739 20 1.108174087859712 21 0.013572923166940391 22 0.004805611829400312 23 0.004852495847248119 24 0.00112521642834739 25 2.813041070868475E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4265846.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.36035395844488 #Duplication Level Percentage of deduplicated Percentage of total 1 67.44003931211871 24.52143700360074 2 14.38609962220495 10.461673486896444 3 6.3301687913673295 6.905015336124521 4 3.4519187088965473 5.020519443650261 5 2.075850123663395 3.773932263054131 6 1.3676452696728225 2.9836839656937975 7 0.9189622282118918 2.338965432455791 8 0.6838955464117912 1.9893347310509437 9 0.5003686540124577 1.6374223232643241 >10 2.490383720579826 16.238530759712212 >50 0.20484562116770194 5.185966341128413 >100 0.13522941767517244 9.306877602363986 >500 0.0102015768077474 2.4099341367057674 >1k 0.003648245989561155 2.2123324975265426 >5k 3.3780055458899585E-4 0.8003308102311211 >10k+ 4.05360665506795E-4 4.214043866541065 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 53001 1.2424499149758337 No Hit TATCAACGCAGAGTACTTTTTTTTT 40561 0.9508313239624684 No Hit GGTATCAACGCAGAGTACTTTTTTT 35893 0.8414040263056847 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 18194 0.4265039103615086 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 12917 0.3028004292700674 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 10980 0.25739325798446544 No Hit GTGGTATCAACGCAGAGTACATGGG 8814 0.20661786665528947 No Hit ATCAACGCAGAGTACTTTTTTTTTT 6542 0.1533576223801797 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 6317 0.1480831703723013 No Hit GGTATCAACGCAGAGTACATGGGAT 5537 0.12979840341165622 No Hit GTGGTATCAACGCAGAGTACTTTTT 5370 0.12588358792136425 No Hit GGTATCAACGCAGAGTACATGGGAG 4342 0.10178520274759098 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.3442008923903957E-5 0.0 0.0 0.0 0.0 7 4.6884017847807915E-5 0.0 0.0 0.0 0.0 8 4.6884017847807915E-5 0.0 0.0 0.0 0.0 9 4.6884017847807915E-5 0.0 0.0 0.0 0.0 10 4.6884017847807915E-5 0.0 0.0 2.3442008923903957E-5 0.0 11 4.6884017847807915E-5 0.0 0.0 9.376803569561583E-5 0.0 12 9.376803569561583E-5 0.0 0.0 9.376803569561583E-5 0.0 13 1.4065205354342374E-4 0.0 0.0 1.4065205354342374E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGTAT 2525 0.0 14.776255 1 GGTATCA 10120 0.0 14.204332 1 TGGTATC 2690 0.0 13.483464 2 CGGTTTG 235 0.0 12.217607 14 TCTAACG 55 0.003146296 12.051312 3 CTGGTCG 265 0.0 11.786746 9 TCGACGG 75 1.9377378E-4 11.48455 14 GGTCGGT 245 0.0 11.2033415 11 ATCACGC 170 1.2732926E-11 11.139869 3 ACCGTTC 70 0.00153372 10.821586 8 TGGTCGG 280 0.0 10.817019 10 CTCGACG 90 9.8895965E-5 10.5163 13 TAATACC 410 0.0 10.392682 4 TATCACG 170 1.6498234E-9 10.027176 2 GTATCAA 21655 0.0 10.000864 1 TTAGGCA 555 0.0 9.895414 4 AACGCCG 365 0.0 9.858021 5 TAGGCAT 535 0.0 9.73437 5 GACATAT 780 0.0 9.712363 1 TCACGCA 225 3.6379788E-12 9.679309 4 >>END_MODULE