FastQCFastQC Report
Thu 26 May 2016
SRR1364264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4926604
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT145350.2950308163595044No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN113630.23064569427540754No Hit
TATCAACGCAGAGTACTTTTTTTTT101500.20602427148599722No Hit
GGTATCAACGCAGAGTACTTTTTTT77060.15641606266710292No Hit
GTACTTTTTTTTTTTTTTTTTTTTT72750.14766764286311626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA72120.14638887152285834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51700.014.2443711
CGGACGA550.003066200512.09294517
CTCAACG2950.011.9187174
ACATGGT23250.011.8120353
GTATCAA137050.011.5858941
TATAACG3700.011.5568242
TCGGACG600.005870469411.08542516
GGTACGA1158.8079105E-710.74115818
CATGGTA16850.010.3769034
ATAACGC3600.010.2946583
TACATGT28900.010.0612352
AGTATCG2201.8189894E-129.9292415
GTGGTAT13800.09.7778051
CATTCCG4000.09.7351729
TAACGCA4100.09.7345354
TCAACTC29800.09.6940934
GCGAATC800.00451173179.50169615
CGCATTG1606.66987E-89.4978268
GTTCTAG4450.09.3956061
TTGCGCG5600.09.33211818
Produced by FastQC (version 0.11.5)