##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364264_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4926604 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.639808070630398 28.0 16.0 31.0 2.0 31.0 2 24.324600678276557 29.0 22.0 31.0 2.0 34.0 3 23.909206625902954 28.0 16.0 31.0 2.0 34.0 4 26.784920403588355 32.0 19.0 35.0 2.0 37.0 5 27.248131167027022 32.0 25.0 35.0 2.0 37.0 6 27.80351597165106 33.0 27.0 35.0 2.0 37.0 7 27.69282877211158 33.0 26.0 35.0 2.0 37.0 8 26.066300234400817 32.0 17.0 35.0 2.0 37.0 9 25.807111145933387 32.0 13.0 37.0 2.0 39.0 10 27.389518824732008 33.0 19.0 37.0 2.0 39.0 11 27.839331312198016 34.0 24.0 37.0 2.0 39.0 12 26.498289085138566 33.0 17.0 37.0 2.0 39.0 13 26.25491149684448 32.0 17.0 37.0 2.0 39.0 14 26.69527873561585 32.0 17.0 38.0 2.0 40.0 15 26.245047298301223 32.0 13.0 38.0 2.0 40.0 16 26.982990717337948 32.0 17.0 38.0 2.0 40.0 17 27.513758970682442 33.0 17.0 38.0 2.0 40.0 18 28.05396821015044 34.0 22.0 39.0 2.0 40.0 19 28.693105027316992 35.0 25.0 40.0 2.0 41.0 20 28.70722895528035 35.0 25.0 40.0 2.0 41.0 21 28.87124152864732 36.0 26.0 40.0 2.0 41.0 22 28.761767335064885 36.0 25.0 40.0 2.0 41.0 23 28.42668194967568 35.0 24.0 40.0 2.0 41.0 24 28.358827500647504 35.0 24.0 40.0 2.0 41.0 25 28.068559600081517 35.0 22.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.2431954610237455 1101 2 -1.3916435823136872 1101 3 -3.6054736838612556 1101 4 -3.667197336105364 1101 5 -4.42058104251479 1101 6 -2.59124584540287 1101 7 -2.159196113949001 1101 8 -5.689019616585014 1101 9 -6.849629199835832 1101 10 -7.230611738194625 1101 11 -4.336872462176633 1101 12 -1.6953283545499787 1101 13 -1.1731284905693542 1101 14 -7.797272036609193 1101 15 -9.678091756179985 1101 16 -5.162578227257573 1101 17 -3.332151654793467 1101 18 -7.463020980619881 1101 19 -4.401617400908972 1101 20 -3.4292272736261005 1101 21 -3.312381505701808 1101 22 -3.2343771417887908 1101 23 -7.80715030707449 1101 24 -4.929301569640458 1101 25 -4.026125167558526 1102 1 1.1785743964932678 1102 2 0.6557026712388598 1102 3 1.1640353912216277 1102 4 1.1730672759672025 1102 5 0.7390862399917104 1102 6 0.18446895108514738 1102 7 -0.022742803508947418 1102 8 1.3120891936971688 1102 9 -3.8616032181627773 1102 10 -1.2829351378049125 1102 11 -0.5628953965941932 1102 12 -4.584439221928676 1102 13 -6.399546644962683 1102 14 -6.634975064935453 1102 15 -1.8256556579750942 1102 16 -0.29852349590241545 1102 17 -4.712062342377642 1102 18 -2.232301805384587 1102 19 -1.4199096070042252 1102 20 -0.9310926133318631 1102 21 -0.7248610160176696 1102 22 -0.7675686765077216 1102 23 -0.5293363280505794 1102 24 -0.5329068582989116 1102 25 -0.2655749174642352 1103 1 -1.2614783717154054 1103 2 0.44892366257721505 1103 3 1.3101977570672965 1103 4 2.0030990214505913 1103 5 1.661480302018159 1103 6 1.014677188579725 1103 7 0.9671482346821101 1103 8 2.284486704431764 1103 9 3.323717628634377 1103 10 1.795778824057443 1103 11 1.357943844094251 1103 12 2.6020933717180235 1103 13 2.7230089151054955 1103 14 -3.160538474282408 1103 15 0.8474165171457138 1103 16 1.4973061534754315 1103 17 1.5574957526040372 1103 18 1.3627004680980406 1103 19 0.9608324831356079 1103 20 1.0273266968070693 1103 21 0.8179811587017589 1103 22 0.9372772116816606 1103 23 1.2499805403244721 1103 24 1.0222264694241758 1103 25 1.0255259689429828 1104 1 1.1953281852846338 1104 2 0.6906556585168317 1104 3 1.227506973028465 1104 4 1.499555615589042 1104 5 0.7047544498941143 1104 6 0.1113759585999432 1104 7 0.02014410429075042 1104 8 1.298100396139187 1104 9 -3.648094852748521 1104 10 -1.2672006332577261 1104 11 -0.6067789910762684 1104 12 0.7309131988360384 1104 13 0.9870571214088493 1104 14 0.6930119319269288 1104 15 -4.247227428302907 1104 16 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2.0068268319638065 1106 4 2.2119835173917792 1106 5 1.2843229225308619 1106 6 0.7996903908349893 1106 7 0.8183586318931297 1106 8 2.3483424061091043 1106 9 3.25982767749694 1106 10 1.5980596276351875 1106 11 0.7195348990309753 1106 12 1.818552737174766 1106 13 -3.9784483058137745 1106 14 0.13536373138282443 1106 15 2.151130573138822 1106 16 1.6275646937049544 1106 17 1.2592973158086203 1106 18 1.0013081065278975 1106 19 0.7749804370833857 1106 20 0.4178791881977162 1106 21 -0.07714973541741088 1106 22 0.16046580680478328 1106 23 0.45906294650774626 1106 24 0.4649220690819291 1106 25 0.4115059799840566 1107 1 1.940056189342922 1107 2 1.3689037065903058 1107 3 2.1386331914231143 1107 4 2.103917006721936 1107 5 1.7779220460308558 1107 6 0.9170318268314759 1107 7 0.5620137581454365 1107 8 2.2608996206584955 1107 9 3.5047221363765573 1107 10 1.9369650321026413 1107 11 0.9472060530432529 1107 12 2.5134579333658102 1107 13 2.7504694787815254 1107 14 -3.150864821749842 1107 15 1.2638054969851709 1107 16 1.5088796186610054 1107 17 1.2247819189595042 1107 18 1.308465126289157 1107 19 1.0747336290202938 1107 20 1.1248471805544291 1107 21 0.7756550268247651 1107 22 0.5626140920517493 1107 23 1.0147570237612165 1107 24 1.1559743199086299 1107 25 1.159828291247667 1108 1 1.4306057758100508 1108 2 0.9096537205469524 1108 3 1.1839803914502518 1108 4 1.6054147603556892 1108 5 0.9462161295037319 1108 6 0.314700344194879 1108 7 0.07040642561245036 1108 8 -4.091567643705815 1108 9 -5.177920516209092 1108 10 -2.005151105746915 1108 11 -0.5204477895631285 1108 12 0.5212097311576329 1108 13 1.7690137607452634 1108 14 2.197173568553783 1108 15 -3.3261862489759046 1108 16 -0.003765380860649259 1108 17 0.5208200489680301 1108 18 0.43112077619214517 1108 19 0.2464481015730442 1108 20 0.23999926678978056 1108 21 -0.07682806343921555 1108 22 -0.18277097388426 1108 23 0.18752913669768745 1108 24 0.17471873741499166 1108 25 0.47709596737070825 1109 1 0.7668786266166947 1109 2 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1110 14 1.5693792700779596 1110 15 2.2251556959766 1110 16 1.7844055718288914 1110 17 1.2143958426155592 1110 18 0.7622860561975955 1110 19 0.14362869184612492 1110 20 0.11989260339656127 1110 21 -0.10116045853352063 1110 22 -0.3989972411286331 1110 23 -0.03337054743491663 1110 24 0.15680813852410225 1110 25 0.37957891109817155 1111 1 1.200010758475429 1111 2 1.0432310571713508 1111 3 1.2357892554536534 1111 4 1.8684344360147165 1111 5 -1.2376742176324527 1111 6 -0.18416382163629663 1111 7 -0.5796877518817176 1111 8 1.7385231240348489 1111 9 3.079980167877501 1111 10 1.7184428793251172 1111 11 1.4427434609019407 1111 12 2.6415772913306377 1111 13 2.8109217032838814 1111 14 3.0543735649214874 1111 15 3.3252435389428 1111 16 2.502850282748877 1111 17 1.937837340869148 1111 18 1.2247310183447553 1111 19 0.8986316547689732 1111 20 0.9796157935810967 1111 21 0.6975231209641173 1111 22 0.6685877028960476 1111 23 1.0297225981308316 1111 24 0.7958400756549935 1111 25 0.9607894798989953 1112 1 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14 3.4714800128290015 1203 15 4.489247397505643 1203 16 3.9014510617756137 1203 17 2.929678296002681 1203 18 2.7411625147766863 1203 19 2.255475910249693 1203 20 2.2743232767586683 1203 21 2.0538525754999846 1203 22 1.822660495925387 1203 23 2.3837514442852736 1203 24 2.332463174768563 1203 25 2.6002380685217794 1204 1 0.4322758366745916 1204 2 0.49662128986415865 1204 3 -2.093457267526272 1204 4 0.056732510648366485 1204 5 -2.3632248542792524 1204 6 -1.1827378541680176 1204 7 -0.860501635465269 1204 8 1.523311597432226 1204 9 2.744209908343155 1204 10 1.448432170429168 1204 11 0.7275663945451143 1204 12 1.7441090081787856 1204 13 1.889129768005219 1204 14 -6.113378262376749 1204 15 -0.3109180566364991 1204 16 -4.141666673877033 1204 17 -1.4632511908734216 1204 18 -0.12403300126312189 1204 19 0.07939887967913606 1204 20 -0.28923954817527076 1204 21 0.05629267293971907 1204 22 0.107476801959141 1204 23 0.33681705129230366 1204 24 -0.33406034297456344 1204 25 -0.25552533642103015 1205 1 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1.8285595097908782 2204 7 1.8527063302885942 2204 8 3.413671871611939 2204 9 -2.213252981705999 2204 10 0.5347568106544394 2204 11 1.6330306834466555 2204 12 3.310063150948068 2204 13 3.506198259503794 2204 14 3.7299962473425268 2204 15 4.220485705087793 2204 16 3.8136654151500657 2204 17 2.8108094427761223 2204 18 2.7593654608510327 2204 19 2.249834359113237 2204 20 2.161862904694768 2204 21 1.765430735178775 2204 22 1.6431683240348178 2204 23 2.0730420770053613 2204 24 2.1748223503021755 2204 25 2.0832421896448423 2205 1 -2.9283326453467673 2205 2 -3.289074834041841 2205 3 -0.040840007408444734 2205 4 -1.7199950935094286 2205 5 -2.3532349844545166 2205 6 -0.780320593633558 2205 7 -0.5882850043569441 2205 8 -4.321062197334861 2205 9 0.9505804492494221 2205 10 0.5185287679671191 2205 11 0.3630871856668314 2205 12 1.9004960487596527 2205 13 -4.26257301924505 2205 14 -0.26951353740665596 2205 15 2.123833640109652 2205 16 1.781015997984568 2205 17 1.696963100258774 2205 18 0.5172164915847937 2205 19 0.6209291181971572 2205 20 0.6171601321488929 2205 21 0.5235749149152582 2205 22 0.5226568824589286 2205 23 0.7117930553860354 2205 24 0.38102249176388625 2205 25 0.8492028711114266 2206 1 -6.749780453335369 2206 2 -5.287182028210484 2206 3 -2.2291130036091893 2206 4 -1.0301909580180215 2206 5 -0.8343177560291579 2206 6 -1.2453880618972981 2206 7 -1.680530500529727 2206 8 -2.980334983597416 2206 9 -5.516594961382761 2206 10 -2.5630054838657337 2206 11 -1.8408851009402412 2206 12 -7.308172956688718 2206 13 -6.378958329726952 2206 14 -7.437229931665534 2206 15 -2.4673314082268867 2206 16 -6.180230123218237 2206 17 -2.920997625976071 2206 18 -2.123801370489243 2206 19 -2.0602000246957495 2206 20 -2.0782415102569907 2206 21 -1.949115954105359 2206 22 -1.9973917233723881 2206 23 -1.565080297056607 2206 24 -1.4859777785630541 2206 25 -1.7811588853011777 2207 1 -6.580339983785446 2207 2 -4.800286523075204 2207 3 -0.9650485674097773 2207 4 -0.13646067057972644 2207 5 0.2644303331350777 2207 6 -0.04687745401242793 2207 7 -0.07931777572057541 2207 8 1.3364661416308898 2207 9 -1.8519839499024968 2207 10 -0.7239456357400194 2207 11 -0.4857431239690122 2207 12 -4.117608931218832 2207 13 -4.693902810157976 2207 14 -1.121735877550563 2207 15 -4.703438604756183 2207 16 -1.0134987754825744 2207 17 -0.18274809628593403 2207 18 0.02520834226891111 2207 19 -0.18521703287880342 2207 20 -0.2889940680757661 2207 21 -0.3950480128761278 2207 22 -0.3576962784119537 2207 23 -0.42598499798399203 2207 24 -0.5764304236973956 2207 25 -0.11164225372789005 2208 1 -3.9349555030477497 2208 2 -4.186327204398069 2208 3 -1.4638203394624654 2208 4 -0.3824980117140804 2208 5 -2.8356831246120073 2208 6 -1.318742188661144 2208 7 -1.0156504991269841 2208 8 -5.082407140026373 2208 9 -8.117148878206553 2208 10 -4.058140437707472 2208 11 -1.9213039425655936 2208 12 -0.37106215228025974 2208 13 0.4744968096180173 2208 14 0.44334194364494195 2208 15 0.8743992326347474 2208 16 -4.856622617105671 2208 17 -6.999555374495291 2208 18 -3.1340640234113373 2208 19 -2.6739381209960023 2208 20 -1.7788969427638932 2208 21 -1.6057677148387128 2208 22 -1.3372170960069525 2208 23 -1.1622117755241312 2208 24 -1.1335430409642342 2208 25 -0.9224089049455948 2209 1 0.942151812968298 2209 2 0.8240545986603998 2209 3 -2.123281284412542 2209 4 0.3362844603198951 2209 5 0.6813669181850806 2209 6 0.2857231416870327 2209 7 0.4200093009909658 2209 8 1.8632228646744409 2209 9 -3.294106167321374 2209 10 -0.4283991462342698 2209 11 0.19165552808955866 2209 12 -3.946937607634812 2209 13 0.27500493450634167 2209 14 1.439556178964711 2209 15 2.307047029326661 2209 16 -2.8988976636879933 2209 17 -0.025052327694609744 2209 18 0.4173580308026459 2209 19 0.3633172855423652 2209 20 0.48697408637950446 2209 21 0.4798775246912186 2209 22 0.3802758486426647 2209 23 0.8296997612128791 2209 24 0.29014660873661313 2209 25 0.5085835331267283 2210 1 -0.2691939234544627 2210 2 -2.762007283898498 2210 3 -0.8713824420135765 2210 4 -3.0765612942623797 2210 5 -3.3011758287029096 2210 6 -1.571946522378834 2210 7 -0.8983681937486416 2210 8 -4.763210200245307 2210 9 -4.538748054649606 2210 10 -1.9541226152065114 2210 11 -0.852507895907145 2210 12 0.6744365542941928 2210 13 -6.73667121123939 2210 14 -1.5836209694020909 2210 15 -4.880386567615258 2210 16 -1.3897606357427037 2210 17 -0.8090511861321339 2210 18 -0.3320939460083565 2210 19 -0.703140720321425 2210 20 -0.5342229944714418 2210 21 -0.7914553536688445 2210 22 -0.5454054921320335 2210 23 -0.1717991260827887 2210 24 -0.31552044092219944 2210 25 -0.21820506412441887 2211 1 0.32257418208599375 2211 2 0.18369931920074478 2211 3 -3.290085558420497 2211 4 -2.784889395457448 2211 5 -0.7521574193753935 2211 6 -0.861770386107235 2211 7 -1.0258164594961308 2211 8 -4.67117823802986 2211 9 0.25890615682827445 2211 10 0.09495581084262028 2211 11 -5.644200907860974 2211 12 -0.5041987065288787 2211 13 0.6685286042939893 2211 14 1.2099206314157769 2211 15 -3.7854769639660546 2211 16 -0.7399610675954378 2211 17 -5.857152915605919 2211 18 -2.025972115801544 2211 19 -1.1882727947203762 2211 20 -0.7246947480548265 2211 21 -0.6700173899381419 2211 22 -0.4068515254697651 2211 23 -0.24679651520541412 2211 24 -0.2278119208874827 2211 25 0.005146046249539182 2212 1 2.279817348088198 2212 2 1.4712511748917159 2212 3 2.4430116529324586 2212 4 2.820282868049606 2212 5 -0.5628474353380888 2212 6 0.8254672259521136 2212 7 1.3733308041463346 2212 8 -1.1414230418283289 2212 9 2.9871194442829854 2212 10 2.4274277246629197 2212 11 2.167816808384206 2212 12 3.6481222136702804 2212 13 3.711034873920113 2212 14 3.9899357455228746 2212 15 4.544476742811842 2212 16 3.7196844018737245 2212 17 2.95863755981841 2212 18 2.7561821740751533 2212 19 2.1232944723846536 2212 20 2.0250320184189796 2212 21 1.8333122675148843 2212 22 1.8186596188270272 2212 23 1.9440503572637375 2212 24 2.0575666800726076 2212 25 2.4104945022494135 2213 1 2.483234651251525 2213 2 2.093445579886893 2213 3 2.602288314337777 2213 4 2.996246987076759 2213 5 1.7958984299175782 2213 6 -0.0956335367295793 2213 7 1.1284509366810127 2213 8 -5.439151168007047 2213 9 1.1626369384928097 2213 10 1.3172139102571556 2213 11 1.3696585629365323 2213 12 2.9505467603318145 2213 13 -1.558362836530506 2213 14 1.4002849889976794 2213 15 -3.231801622356077 2213 16 0.38176756365150055 2213 17 1.5677186896726418 2213 18 1.710043943102221 2213 19 1.637356642447795 2213 20 1.9599672860261492 2213 21 1.7231669128308447 2213 22 1.7528013665264197 2213 23 2.068510061718314 2213 24 2.135977018869532 2213 25 2.3302194543485015 2214 1 2.0419195807256365 2214 2 1.2195750206599847 2214 3 0.7970473534212559 2214 4 1.6270131061912139 2214 5 1.4940125052143074 2214 6 0.040582864630728466 2214 7 0.478159086136138 2214 8 1.771452057543673 2214 9 2.542362295086104 2214 10 1.3185611082276694 2214 11 1.3299360420145625 2214 12 2.295483308374589 2214 13 0.5062670931163886 2214 14 2.1820262131539394 2214 15 3.35672951006266 2214 16 2.1285858184635025 2214 17 1.5241568323994024 2214 18 1.088065101885583 2214 19 1.0029038965219144 2214 20 0.5906175390005579 2214 21 0.35961352191678486 2214 22 0.4074750407230283 2214 23 -5.64393177652849 2214 24 -2.2385036162395338 2214 25 -0.6781102031145991 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 635063.0 3 10.0 4 4534.0 5 27542.0 6 38597.0 7 35585.0 8 30096.0 9 24716.0 10 21860.0 11 19755.0 12 18484.0 13 17296.0 14 16625.0 15 16048.0 16 15962.0 17 17207.0 18 21323.0 19 30020.0 20 42562.0 21 58719.0 22 75113.0 23 90297.0 24 105556.0 25 120223.0 26 134128.0 27 146077.0 28 157300.0 29 179188.0 30 192128.0 31 206053.0 32 250388.0 33 300956.0 34 349135.0 35 387844.0 36 413189.0 37 390554.0 38 336471.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.579473166252754 19.07934740404832 15.619340192053945 21.721839237644982 2 14.356889542427126 22.260093519286446 41.45090237461648 21.932114563669945 3 15.536003487783498 29.61277990958548 31.661832360467795 23.189384242163225 4 9.431415212296407 18.239003702144483 40.97940123307053 31.350179852488573 5 10.540876519067051 40.27891741237954 35.5758221093885 13.604383959164904 6 27.359411828904566 39.44527881440553 19.92271173133215 13.27259762535775 7 25.92581280762099 34.830947294991795 22.727318077429526 16.515921819957693 8 24.405011207801746 34.276754210841055 23.921657577783204 17.396577003573995 9 25.95777617180317 16.64079527102247 23.89289058934856 33.508537967825795 10 14.464812171198044 31.987959767885698 32.466172734530716 21.081055326385542 11 31.583395774624947 26.12483914580313 24.32076770210617 17.970997377465753 12 21.813543433040287 28.708692309321236 31.49314871012984 17.98461554750863 13 26.52123186561466 24.321201323491984 28.197078136930514 20.96048867396284 14 21.115454053280345 23.453190800129576 30.372415944319126 25.058939202270953 15 21.845055444670063 30.332366685106482 27.10864948254831 20.713928387675143 16 21.237011513490433 30.3894698199219 27.37890392678701 20.994614739800653 17 20.16542296078351 30.11618983978723 28.209757812787906 21.508629386641353 18 19.992649880840517 29.15824847271243 30.20074376690553 20.648357879541525 19 21.83683233303745 28.908116647800842 29.58753063999935 19.667520379162358 20 22.550456196611226 27.9773769499416 29.35213848399411 20.12002836945306 21 23.601068249427325 27.534175817307116 28.502335395871032 20.36242053739453 22 22.55292592965878 27.886006786328387 28.769043917704824 20.79202336630801 23 21.823370350913628 28.107847808469653 29.0481106373621 21.020671203254615 24 21.441799520879805 28.203319633034248 29.38309966127648 20.971781184809466 25 21.406498060868394 27.830687248929443 29.71449654352616 21.04831814667601 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 20813.0 1 20813.0 2 17540.0 3 14267.0 4 14267.0 5 14267.0 6 19545.5 7 24824.0 8 24824.0 9 24824.0 10 28324.0 11 31824.0 12 31824.0 13 31824.0 14 41849.5 15 51875.0 16 51875.0 17 51875.0 18 80601.0 19 109327.0 20 109327.0 21 109327.0 22 153588.0 23 197849.0 24 197849.0 25 197849.0 26 262028.5 27 326208.0 28 326208.0 29 326208.0 30 378420.0 31 430632.0 32 430632.0 33 430632.0 34 470481.5 35 510331.0 36 510331.0 37 510331.0 38 537977.0 39 565623.0 40 565623.0 41 565623.0 42 582651.0 43 599679.0 44 599679.0 45 599679.0 46 594534.0 47 589389.0 48 589389.0 49 589389.0 50 546700.5 51 504012.0 52 504012.0 53 504012.0 54 441598.0 55 379184.0 56 379184.0 57 379184.0 58 318510.0 59 257836.0 60 257836.0 61 257836.0 62 207145.0 63 156454.0 64 156454.0 65 156454.0 66 119482.5 67 82511.0 68 82511.0 69 82511.0 70 61668.5 71 40826.0 72 40826.0 73 40826.0 74 29424.5 75 18023.0 76 18023.0 77 18023.0 78 13406.0 79 8789.0 80 8789.0 81 8789.0 82 6241.0 83 3693.0 84 3693.0 85 3693.0 86 2728.5 87 1764.0 88 1764.0 89 1764.0 90 1251.0 91 738.0 92 738.0 93 738.0 94 424.5 95 111.0 96 111.0 97 111.0 98 66.5 99 22.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2551047334025629 2 0.26750678560728647 3 0.273109021955083 4 0.3399298989730045 5 0.2835015763394014 6 0.31218259068518595 7 0.3110662030071831 8 0.3104166683581632 9 0.3202002840090253 10 0.31264944371416903 11 0.31104590504940116 12 0.31283212533420585 13 0.3116548437828573 14 0.30826508483328474 15 0.3071283991974999 16 0.30923938680681456 17 0.3059511176461514 18 0.30688482370411746 19 0.2988671303802782 20 0.34705448215444146 21 0.30481443201036656 22 0.3097265377935795 23 0.31307570082758834 24 0.31500400681686613 25 0.31228408047409534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4926604.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.52732186378024 #Duplication Level Percentage of deduplicated Percentage of total 1 95.88229555855492 81.0467365771608 2 2.498376465740039 4.223621433130041 3 0.6495057201799513 1.647029371860514 4 0.31056805178668334 1.0500594269592063 5 0.16732076279969343 0.7071587985831458 6 0.10037263054982444 0.5090537789279589 7 0.07208588326292682 0.42652586594802144 8 0.051647683171070796 0.3492512271135718 9 0.03191000055039059 0.2427540198476643 >10 0.19770148089319237 3.0624426327181338 >50 0.020341489664548382 1.2130208227327905 >100 0.015798045608994355 2.7013204281444985 >500 0.0012976436342202128 0.7502141785319228 >1k 6.37022948587166E-4 0.9093167015988706 >5k 7.07803276180186E-5 0.44259289319441547 >10k+ 7.07803276180186E-5 0.7189018435485193 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 14535 0.2950308163595044 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 11363 0.23064569427540754 No Hit TATCAACGCAGAGTACTTTTTTTTT 10150 0.20602427148599722 No Hit GGTATCAACGCAGAGTACTTTTTTT 7706 0.15641606266710292 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 7275 0.14766764286311626 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 7212 0.14638887152285834 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.029795778187165E-5 0.0 8 0.0 0.0 0.0 2.029795778187165E-5 0.0 9 0.0 0.0 0.0 6.089387334561495E-5 0.0 10 0.0 0.0 0.0 6.089387334561495E-5 0.0 11 0.0 0.0 0.0 1.0148978890935825E-4 0.0 12 4.05959155637433E-5 0.0 0.0 1.0148978890935825E-4 0.0 13 4.05959155637433E-5 0.0 0.0 1.217877466912299E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5170 0.0 14.244371 1 CGGACGA 55 0.0030662005 12.092945 17 CTCAACG 295 0.0 11.918717 4 ACATGGT 2325 0.0 11.812035 3 GTATCAA 13705 0.0 11.585894 1 TATAACG 370 0.0 11.556824 2 TCGGACG 60 0.0058704694 11.085425 16 GGTACGA 115 8.8079105E-7 10.741158 18 CATGGTA 1685 0.0 10.376903 4 ATAACGC 360 0.0 10.294658 3 TACATGT 2890 0.0 10.061235 2 AGTATCG 220 1.8189894E-12 9.929241 5 GTGGTAT 1380 0.0 9.777805 1 CATTCCG 400 0.0 9.735172 9 TAACGCA 410 0.0 9.734535 4 TCAACTC 2980 0.0 9.694093 4 GCGAATC 80 0.0045117317 9.501696 15 CGCATTG 160 6.66987E-8 9.497826 8 GTTCTAG 445 0.0 9.395606 1 TTGCGCG 560 0.0 9.332118 18 >>END_MODULE