FastQCFastQC Report
Thu 26 May 2016
SRR1364264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4926604
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT469400.9527861382810553No Hit
TATCAACGCAGAGTACTTTTTTTTT388790.7891643006013879No Hit
GGTATCAACGCAGAGTACTTTTTTT304080.6172203002311532No Hit
GTACTTTTTTTTTTTTTTTTTTTTT214560.4355129821678382No Hit
ACGCAGAGTACTTTTTTTTTTTTTT153700.31197961110736727No Hit
GAGTACTTTTTTTTTTTTTTTTTTT136970.27802112773829596No Hit
GCAGAGTACTTTTTTTTTTTTTTTT60760.12333039148265214No Hit
GTGGTATCAACGCAGAGTACATGGG60690.12318830577817905No Hit
ATCAACGCAGAGTACTTTTTTTTTT59270.12030599577315328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87850.014.1334781
TATCACG3900.014.0977262
ATCACGC4100.013.87019353
TATAACG5100.012.0817222
ACGCGAG550.003122496412.0636017
ATAACGC5500.011.8905383
TAACGCA5300.011.4453224
TCACGCA5050.011.2611774
GCCCCCG2900.011.197371518
GTGGTAT21950.011.0541411
GTGCACG4050.010.99476611
TTCGCAA700.001427325210.91530917
TGGTATC21700.010.8337212
GTTCGCG803.5919715E-410.74475816
TACCCCG1159.0937647E-710.7145485
CGTCCGA1355.929178E-810.52690411
GTATCAA191050.010.3536841
TTCGCGC750.00253442910.18762217
CGCAATA750.0025352910.18720519
TCGTCCG1502.554043E-810.10603310
Produced by FastQC (version 0.11.5)