##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364264_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4926604 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.12307666701038 31.0 28.0 33.0 25.0 34.0 2 29.171191961034417 31.0 28.0 34.0 25.0 34.0 3 29.23768888264614 31.0 28.0 34.0 25.0 34.0 4 32.70740087898277 35.0 32.0 37.0 26.0 37.0 5 32.03313255946693 35.0 32.0 37.0 22.0 37.0 6 32.07054981484203 35.0 32.0 37.0 23.0 37.0 7 31.663275757499488 35.0 32.0 37.0 18.0 37.0 8 31.45904846421592 35.0 32.0 37.0 17.0 37.0 9 31.830572134476405 37.0 30.0 39.0 10.0 39.0 10 31.841878096960908 37.0 30.0 39.0 11.0 39.0 11 32.409279901530546 37.0 32.0 39.0 17.0 39.0 12 32.2717435783351 37.0 32.0 39.0 15.0 39.0 13 32.343247600172454 37.0 32.0 39.0 15.0 39.0 14 32.80677074918138 38.0 31.0 40.0 10.0 41.0 15 33.08594683071747 38.0 32.0 40.0 12.0 41.0 16 33.112857863144676 38.0 32.0 40.0 11.0 41.0 17 33.152354847274104 38.0 32.0 40.0 11.0 41.0 18 33.069528015647286 38.0 32.0 40.0 10.0 41.0 19 32.95757178778729 38.0 32.0 40.0 10.0 41.0 20 32.67021725310173 38.0 32.0 40.0 2.0 41.0 21 32.275090914552905 38.0 31.0 40.0 2.0 41.0 22 32.32305843944429 38.0 31.0 40.0 2.0 41.0 23 32.19880408492341 38.0 31.0 40.0 2.0 41.0 24 32.19817951676246 38.0 31.0 40.0 2.0 41.0 25 32.10969097577154 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7947088821042669 1101 2 0.7819614753586137 1101 3 0.7906345298867059 1101 4 0.7507663820271375 1101 5 1.00906962093487 1101 6 0.9134565586178205 1101 7 1.1983206571166214 1101 8 1.2237355399538536 1101 9 2.0820146354631923 1101 10 2.223033943215871 1101 11 1.376169656891939 1101 12 1.463728699461953 1101 13 1.1495497976477722 1101 14 1.8019582841402695 1101 15 1.5051345033688577 1101 16 1.3533130363189372 1101 17 1.243829004930717 1101 18 1.3616726107918993 1101 19 1.3409019760886096 1101 20 2.0956962580359857 1101 21 2.4001883284063403 1101 22 2.307937634259357 1101 23 2.1409511883638217 1101 24 1.9827851234309186 1101 25 2.1150411455070213 1102 1 0.3141826722282879 1102 2 0.5309392068291281 1102 3 0.4786957775609544 1102 4 0.44122468458980535 1102 5 0.7659007557948847 1102 6 0.5826317038643616 1102 7 0.8462305363458533 1102 8 0.8794824478871739 1102 9 1.9075109637895231 1102 10 1.9564374530953295 1102 11 1.0712648864697698 1102 12 1.1124465781823574 1102 13 0.810680280335589 1102 14 1.5239378561159427 1102 15 1.0435486622441559 1102 16 0.94923267324927 1102 17 0.9538467388808982 1102 18 0.8553587595900609 1102 19 0.9328195308983069 1102 20 1.5062500721717882 1102 21 2.1023868064658267 1102 22 1.9224656674838023 1102 23 1.7565743349556016 1102 24 1.7188743897796108 1102 25 1.7977877058515048 1103 1 0.4530082594585849 1103 2 0.5630821660243797 1103 3 0.6127809435454097 1103 4 0.6137817661818659 1103 5 0.8312066186153864 1103 6 0.7195026147094588 1103 7 0.9720485219762445 1103 8 0.9214444347246946 1103 9 1.6501534322546583 1103 10 1.8403250053669318 1103 11 1.017767643021358 1103 12 1.0546341074395755 1103 13 0.8961517829785279 1103 14 1.527726640907538 1103 15 1.0663213422230058 1103 16 0.8880339850954968 1103 17 0.8225057643333145 1103 18 0.4771679164324283 1103 19 0.5612274737430667 1103 20 1.1619464803119186 1103 21 1.8698328978280152 1103 22 1.4174023001185425 1103 23 1.4633019758291397 1103 24 1.4325868959040697 1103 25 1.227179231203813 1104 1 0.23780686701332243 1104 2 0.4686973241236032 1104 3 0.27252871795787215 1104 4 0.28157983846251966 1104 5 0.5641675336865326 1104 6 0.30911320980940005 1104 7 0.5249821469208733 1104 8 0.6895537495422808 1104 9 1.3656898048835409 1104 10 1.5185043225814603 1104 11 0.9112817298145472 1104 12 0.6099770963165199 1104 13 0.6811991529994472 1104 14 1.1953640480901768 1104 15 0.3454602853925621 1104 16 0.441313261595468 1104 17 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2.289316603229647 2203 25 2.5035421477626443 2204 1 0.7904121473779 2204 2 0.8357093113203042 2204 3 0.8199722192599097 2204 4 0.8390454591724676 2204 5 1.1094935164766078 2204 6 1.010961875061593 2204 7 1.3242014996463816 2204 8 1.1184022248059797 2204 9 2.1904430438632296 2204 10 2.424405765073619 2204 11 1.4703790304241053 2204 12 1.6530881235708605 2204 13 1.2413702746722564 2204 14 2.0404025624659567 2204 15 1.7927779800895394 2204 16 1.7556451717786743 2204 17 1.6154887816755803 2204 18 1.6889879589568437 2204 19 1.7646949910792813 2204 20 2.4382163458044843 2204 21 2.721666611793811 2204 22 2.6113307136713892 2204 23 2.6905163535220282 2204 24 2.3552078953355853 2204 25 2.6076282516688636 2205 1 0.41200964451403266 2205 2 0.5662555929082771 2205 3 0.6084889399682147 2205 4 0.5145645133864178 2205 5 0.7281801278937259 2205 6 0.38138054423768963 2205 7 0.36084101354963494 2205 8 0.8514213532378498 2205 9 1.5295533076596364 2205 10 1.7013497326646565 2205 11 1.0047992635956007 2205 12 1.1142839910228446 2205 13 0.9727547357488433 2205 14 1.0729659810894177 2205 15 0.1588135627520444 2205 16 0.15069665074154415 2205 17 0.1389064202828436 2205 18 0.5970682641597804 2205 19 0.6331827102518659 2205 20 1.2229322961543403 2205 21 1.2755100162239863 2205 22 0.781134092730813 2205 23 0.06544288956201783 2205 24 0.14360064855652155 2205 25 0.37368645312598403 2206 1 -1.074487851324868 2206 2 -3.082172072199512 2206 3 -0.9506050787847542 2206 4 -1.9543035408809537 2206 5 -0.40702765757388093 2206 6 -0.6434494863245774 2206 7 -0.5052128086023941 2206 8 0.18296249417271326 2206 9 1.387492149115051 2206 10 0.8145611423624324 2206 11 0.5664250839111418 2206 12 0.3708281368475781 2206 13 0.34619220756285074 2206 14 0.8344653212421136 2206 15 0.06464307496676724 2206 16 0.5763172954696785 2206 17 0.6975428940402182 2206 18 0.10832566461031234 2206 19 0.5184154561871495 2206 20 1.1492698665494245 2206 21 1.5896701241271778 2206 22 1.3502707476221012 2206 23 1.1353728806388332 2206 24 0.9516760856136308 2206 25 0.7857685091521347 2207 1 0.40432735812651543 2207 2 0.44318601725939644 2207 3 0.2910425592232109 2207 4 0.37178835603808125 2207 5 0.5639401106268132 2207 6 0.5269291861376253 2207 7 0.34334520506929067 2207 8 0.6103726028234675 2207 9 1.069780090933623 2207 10 1.5538288108403755 2207 11 0.4182284036603292 2207 12 0.5493658776955996 2207 13 0.5432927646971635 2207 14 1.4683429078171244 2207 15 0.8025680792188155 2207 16 -0.06672635766057766 2207 17 0.10802648734716058 2207 18 0.6284303709141454 2207 19 0.871023666228389 2207 20 1.4901761364687758 2207 21 1.7283034881855208 2207 22 1.5220187597583994 2207 23 0.7620599217991568 2207 24 1.0884744140556073 2207 25 0.9958018767258494 2208 1 0.16785650351542358 2208 2 0.4115022976783784 2208 3 0.4246702968667577 2208 4 0.3100204278006373 2208 5 0.40496054294264283 2208 6 0.40613115037634984 2208 7 0.7231688010680593 2208 8 0.641347205579617 2208 9 1.5020515325107944 2208 10 1.6223566640912956 2208 11 0.8117442213663537 2208 12 0.9085673883867997 2208 13 0.7189041325823098 2208 14 1.3766213548137216 2208 15 0.8959474074676947 2208 16 0.754995285107448 2208 17 0.6016199041993815 2208 18 0.3209753725133737 2208 19 0.6431543167336429 2208 20 1.441257835495584 2208 21 1.9799731099977613 2208 22 1.6887466080958866 2208 23 1.6797252079805531 2208 24 1.3990122009421277 2208 25 1.1651567691571358 2209 1 0.37537018449954473 2209 2 0.3389029038536826 2209 3 0.3506444042156609 2209 4 0.36881433409546815 2209 5 0.6132988481553063 2209 6 0.346597171290842 2209 7 0.611986951260949 2209 8 0.5863295212594899 2209 9 1.4397389747208074 2209 10 1.4695582379747911 2209 11 0.3930065398904006 2209 12 0.8914089668772149 2209 13 0.6715157882528118 2209 14 1.0762388775141503 2209 15 0.7431234908856155 2209 16 0.5459188298000299 2209 17 0.2976586410254285 2209 18 0.4597744844779257 2209 19 0.40890433399072634 2209 20 1.1706037050894267 2209 21 1.8198841866088635 2209 22 1.72318730576038 2209 23 1.608120697236835 2209 24 1.0867045029725126 2209 25 1.1181874073992333 2210 1 -0.06592008047076803 2210 2 0.24925308593214623 2210 3 0.2741995098031502 2210 4 0.34982781052014644 2210 5 0.053810435943514534 2210 6 0.3235658855906216 2210 7 0.49119687346971475 2210 8 0.3851009104229597 2210 9 1.575655083768364 2210 10 1.5587730487061897 2210 11 0.8175282994469697 2210 12 0.6839991058758699 2210 13 0.5579460414978001 2210 14 1.1710662218207162 2210 15 0.6494742157787243 2210 16 0.7666279780943768 2210 17 0.5750035622877476 2210 18 0.5498655269945587 2210 19 0.6417822107370768 2210 20 1.3555027123786658 2210 21 1.7624211468524784 2210 22 1.5610564823629254 2210 23 1.6016096665281125 2210 24 1.6244716015165501 2210 25 1.5120820189597026 2211 1 0.24087033278439307 2211 2 0.1490936689670228 2211 3 0.020320101371893884 2211 4 0.05859492676536604 2211 5 0.39646249018829494 2211 6 0.07828319760202973 2211 7 0.41984306005890915 2211 8 0.4078845593482576 2211 9 1.283373257242399 2211 10 1.2091800756051718 2211 11 0.6196581062245059 2211 12 0.06665079369791016 2211 13 -0.07268924830681556 2211 14 0.6918508824235019 2211 15 0.4414497285871093 2211 16 0.48188465329906904 2211 17 0.15419503805809143 2211 18 0.024247109018723734 2211 19 0.06870833331397108 2211 20 0.7732231328455299 2211 21 1.6245467466908785 2211 22 1.4574820885560449 2211 23 1.4475585793532346 2211 24 1.2872307434984798 2211 25 1.0573174827182044 2212 1 0.5587345092410914 2212 2 0.5123580181907634 2212 3 0.4118503786357124 2212 4 0.5773626796723832 2212 5 0.5722739205377216 2212 6 0.5554915694612212 2212 7 0.8520652470625869 2212 8 0.8296007718260405 2212 9 -9.250611165638041 2212 10 -1.4447947650437136 2212 11 -0.29509752229585473 2212 12 0.7064706280846877 2212 13 0.6492189047047603 2212 14 1.3719709567221514 2212 15 0.987995119958029 2212 16 0.8807864180225309 2212 17 0.8993996843520762 2212 18 1.0867088297439693 2212 19 1.2630525999337223 2212 20 1.7243119243127794 2212 21 2.1611581333871364 2212 22 2.0270498870822244 2212 23 1.863613020029966 2212 24 1.745182822109495 2212 25 1.629998428210527 2213 1 0.4334451398171133 2213 2 0.3832873460291175 2213 3 0.16342578795938323 2213 4 0.42979374498086287 2213 5 0.35375021021301833 2213 6 0.07175754362843278 2213 7 0.45208747732013776 2213 8 0.6983535958766218 2213 9 1.322429437732378 2213 10 -8.509152962465112 2213 11 -2.4212770073896053 2213 12 -0.7010778257981123 2213 13 0.054081898903163506 2213 14 0.6222569136065985 2213 15 0.4442169734712138 2213 16 0.7434784325685868 2213 17 0.489948495924736 2213 18 0.47174503887776353 2213 19 0.937295893666743 2213 20 1.6985494794078804 2213 21 1.6001190833489964 2213 22 1.8375319967392407 2213 23 1.4967024101798998 2213 24 1.5029941947526844 2213 25 1.2199048466263136 2214 1 -2.087109665883503 2214 2 -2.4099203064212453 2214 3 -2.210021199216065 2214 4 -2.2865946566152395 2214 5 -2.364394986322381 2214 6 -2.4171935019110222 2214 7 -10.290968177049134 2214 8 -3.942892818879443 2214 9 -3.2392288811515684 2214 10 -2.846313520411396 2214 11 -3.2386646019882086 2214 12 -11.7921501364332 2214 13 -5.571456777011111 2214 14 -13.393091284471652 2214 15 -7.043321826440156 2214 16 -5.1683400661721315 2214 17 -4.252840615503938 2214 18 -3.8362040389700525 2214 19 -4.160445326424039 2214 20 -4.274236206855072 2214 21 -13.072293036249249 2214 22 -15.42336069088768 2214 23 -9.86781958101021 2214 24 -8.681278704563706 2214 25 -15.825191982910198 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 194307.0 3 8.0 4 2959.0 5 27971.0 6 35815.0 7 29376.0 8 24599.0 9 18678.0 10 15668.0 11 14029.0 12 13386.0 13 12540.0 14 12420.0 15 12485.0 16 13292.0 17 14307.0 18 16323.0 19 20348.0 20 26808.0 21 35962.0 22 45129.0 23 54430.0 24 63142.0 25 72466.0 26 82388.0 27 90656.0 28 100413.0 29 117253.0 30 139292.0 31 168719.0 32 207494.0 33 255687.0 34 310384.0 35 371877.0 36 462660.0 37 624440.0 38 1218893.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.71476024949646 20.791330972646353 14.091372392956922 23.402536384900266 2 15.17717394614237 22.906545945452148 39.24260294968484 22.673677158720654 3 17.336961574233264 28.886309209428607 30.897579598492896 22.87914961784523 4 11.648186052704865 18.975139873227075 38.695600458246695 30.681073615821365 5 11.840263949227042 40.070156678241034 34.550570578690085 13.539008793841834 6 27.72631160590086 39.77404412064702 19.14440102652228 13.35524324692984 7 25.390236243615483 34.36169649169493 23.246532524575432 17.00153474011416 8 25.620537076873305 34.91600774360252 21.181470021361314 18.281985158162858 9 26.353828646778293 15.699536493664088 21.243363764173235 36.70327109538438 10 14.41213969244797 31.20544586762357 32.887995841225255 21.494418598703206 11 32.38015531431998 24.78446215357796 24.372497790981928 18.462884741120135 12 22.292080429886113 28.743335807116377 30.575369727716062 18.38921403528145 13 27.659396102572764 23.35299963544335 27.495487698039923 21.492116563943966 14 21.55841187559385 22.577565070502814 29.898584016655903 25.965439037247435 15 23.133679427888488 30.111748648964635 25.55450096131362 21.20007096183325 16 21.377926296493488 29.3920302067744 27.581158276831967 21.648885219900148 17 20.330693726895323 29.201488604873404 28.718223839600267 21.749593828631 18 19.82938591799506 27.554395493648 31.801803977909927 20.814414610447013 19 21.523061452414176 27.384122347022583 31.52335375238052 19.569462448182726 20 21.910335372715924 26.541343537066975 31.449653168545765 20.09866792167134 21 22.83358389324422 25.82326948708136 30.76994133751283 20.573205282161588 22 22.029510122866146 26.565261397587665 30.707197918709326 20.69803056083687 23 21.347583582751266 26.507814644175475 30.978600672007435 21.166001101065827 24 21.033200594826567 26.851596617299418 31.185422932975648 20.929779854898367 25 21.060324562722883 26.60487515951777 31.49779552373894 20.8370047540204 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2626.0 1 2626.0 2 4515.5 3 6405.0 4 6405.0 5 6405.0 6 17309.5 7 28214.0 8 28214.0 9 28214.0 10 29065.5 11 29917.0 12 29917.0 13 29917.0 14 33444.0 15 36971.0 16 36971.0 17 36971.0 18 66080.0 19 95189.0 20 95189.0 21 95189.0 22 139147.0 23 183105.0 24 183105.0 25 183105.0 26 259068.5 27 335032.0 28 335032.0 29 335032.0 30 383443.0 31 431854.0 32 431854.0 33 431854.0 34 467113.0 35 502372.0 36 502372.0 37 502372.0 38 520868.0 39 539364.0 40 539364.0 41 539364.0 42 558341.0 43 577318.0 44 577318.0 45 577318.0 46 586743.0 47 596168.0 48 596168.0 49 596168.0 50 564771.5 51 533375.0 52 533375.0 53 533375.0 54 467141.5 55 400908.0 56 400908.0 57 400908.0 58 339428.0 59 277948.0 60 277948.0 61 277948.0 62 226149.0 63 174350.0 64 174350.0 65 174350.0 66 133408.0 67 92466.0 68 92466.0 69 92466.0 70 68732.0 71 44998.0 72 44998.0 73 44998.0 74 32263.0 75 19528.0 76 19528.0 77 19528.0 78 15176.0 79 10824.0 80 10824.0 81 10824.0 82 7625.5 83 4427.0 84 4427.0 85 4427.0 86 3253.0 87 2079.0 88 2079.0 89 2079.0 90 1571.0 91 1063.0 92 1063.0 93 1063.0 94 581.5 95 100.0 96 100.0 97 100.0 98 51.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03012216934829753 2 0.01327486438934406 3 0.0013396652136035288 4 0.0 5 0.004100187471938074 6 0.0013599631713854005 7 7.510244379292511E-4 8 0.04629964170044923 9 0.004912105783212939 10 0.0031055875406263626 11 0.004201677260847431 12 0.005602236347796575 13 0.0014614529602947587 14 0.0012787713402579138 15 2.435754933824598E-4 16 5.480448601105346E-4 17 0.002273371271569625 18 9.540040157479675E-4 19 0.0032070773295357208 20 0.8018505242150576 21 0.01966872109063363 22 0.00872812184620481 23 0.009499444241915933 24 0.0025575426805158276 25 7.510244379292511E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4926604.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.623796519555604 #Duplication Level Percentage of deduplicated Percentage of total 1 70.49811802368228 29.343993196293667 2 14.704861250050374 12.241443050407897 3 5.738756218264345 7.166064633131086 4 2.8198742800117653 4.694954929677523 5 1.65178002197448 3.437667776486643 6 1.0388566937456685 2.59446957800688 7 0.6672231717030163 1.9440653072469203 8 0.47896946809445606 1.5949222143234736 9 0.35627943034966963 1.3346732261680048 >10 1.7629141724901862 13.330476801903027 >50 0.1534284629756643 4.503658342935195 >100 0.11715583851404981 9.396167899571811 >500 0.008329339887622694 2.337743084725118 >1k 0.0029965303988744724 2.17253244340596 >5k 1.5236595248513724E-4 0.38204505602163774 >10k+ 3.047319049702745E-4 3.525122459695003 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 46940 0.9527861382810553 No Hit TATCAACGCAGAGTACTTTTTTTTT 38879 0.7891643006013879 No Hit GGTATCAACGCAGAGTACTTTTTTT 30408 0.6172203002311532 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 21456 0.4355129821678382 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 15370 0.31197961110736727 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 13697 0.27802112773829596 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 6076 0.12333039148265214 No Hit GTGGTATCAACGCAGAGTACATGGG 6069 0.12318830577817905 No Hit ATCAACGCAGAGTACTTTTTTTTTT 5927 0.12030599577315328 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.089387334561495E-5 0.0 2 0.0 0.0 0.0 6.089387334561495E-5 0.0 3 0.0 0.0 0.0 6.089387334561495E-5 0.0 4 0.0 0.0 0.0 8.11918311274866E-5 0.0 5 0.0 0.0 0.0 8.11918311274866E-5 0.0 6 0.0 2.029795778187165E-5 0.0 8.11918311274866E-5 0.0 7 0.0 2.029795778187165E-5 0.0 1.0148978890935825E-4 0.0 8 0.0 2.029795778187165E-5 0.0 1.0148978890935825E-4 0.0 9 0.0 2.029795778187165E-5 0.0 1.4208570447310156E-4 0.0 10 0.0 2.029795778187165E-5 0.0 1.8268162003684486E-4 0.0 11 0.0 2.029795778187165E-5 0.0 2.029795778187165E-4 0.0 12 0.0 2.029795778187165E-5 0.0 2.029795778187165E-4 0.0 13 0.0 2.029795778187165E-5 0.0 2.2327753560058816E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8785 0.0 14.133478 1 TATCACG 390 0.0 14.097726 2 ATCACGC 410 0.0 13.8701935 3 TATAACG 510 0.0 12.081722 2 ACGCGAG 55 0.0031224964 12.063601 7 ATAACGC 550 0.0 11.890538 3 TAACGCA 530 0.0 11.445322 4 TCACGCA 505 0.0 11.261177 4 GCCCCCG 290 0.0 11.1973715 18 GTGGTAT 2195 0.0 11.054141 1 GTGCACG 405 0.0 10.994766 11 TTCGCAA 70 0.0014273252 10.915309 17 TGGTATC 2170 0.0 10.833721 2 GTTCGCG 80 3.5919715E-4 10.744758 16 TACCCCG 115 9.0937647E-7 10.714548 5 CGTCCGA 135 5.929178E-8 10.526904 11 GTATCAA 19105 0.0 10.353684 1 TTCGCGC 75 0.002534429 10.187622 17 CGCAATA 75 0.00253529 10.187205 19 TCGTCCG 150 2.554043E-8 10.106033 10 >>END_MODULE