FastQCFastQC Report
Thu 26 May 2016
SRR1364263_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364263_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5035096
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT114400.22720520125137636No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN102600.20376969972369943No Hit
TATCAACGCAGAGTACTTTTTTTTT73200.1453795518496569No Hit
GGTATCAACGCAGAGTACTTTTTTT65700.13048410596342155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50700.013.0271481
AGGACCG2800.011.87268455
GTATCAA129450.011.3569271
TTGACCG8150.011.19112218
GGACCGA2400.011.0810626
TGACCGA8150.010.72514619
ACATGGT23350.010.6232273
GTGGTAT15300.010.4349511
TACATGT28800.010.2951972
TACGCTA750.002653620710.1313575
TCAACTC29800.09.82284454
TATCACG1554.0490704E-89.8098142
CAACTCA31050.09.6358015
TGGTATC16500.09.5608552
TCGACCG800.0045152879.50079718
GCGCGCT1002.764652E-49.4975811
ACGGCCC3250.09.35472415
GCCCCCG3500.09.22934518
CGGCCCC3400.09.22172916
GTACTAG3550.09.1017131
Produced by FastQC (version 0.11.5)