FastQCFastQC Report
Thu 26 May 2016
SRR1364263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5035096
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT365240.7253883540651459No Hit
TATCAACGCAGAGTACTTTTTTTTT274090.5443590350610992No Hit
GGTATCAACGCAGAGTACTTTTTTT243280.48316854336044435No Hit
ACGCAGAGTACTTTTTTTTTTTTTT118510.2353679055970333No Hit
GTGGTATCAACGCAGAGTACATGGG85670.1701457132098375No Hit
GTACTTTTTTTTTTTTTTTTTTTTT75790.15052344582903682No Hit
GAGTACTTTTTTTTTTTTTTTTTTT66020.1311196449879009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG250.006100895318.9637155
GGTATCA84350.013.1407011
AGGACCG3200.013.0375555
GTGGTAT26100.012.9693091
CGCGCCA1551.8189894E-1212.22894410
TGGTATC26000.012.03681852
ACCGAGT3100.011.9289078
GGACCGA3200.011.8524416
GTACCGT658.157988E-411.6700956
CGCGGTT2400.010.73599718
TCGCGGT1451.34750735E-810.53043217
CTGGTCG4450.010.2230519
ACGGCCC3550.010.21526115
GCCCCCG3550.010.21515918
CGAGTGG3400.010.03492810
GTATCAA174800.010.02419951
GTCGGTT3900.09.96335512
CGGTTTG3650.09.93439714
CGGTTGA1553.7953214E-89.85095118
GTCCTAC2600.09.8464471
Produced by FastQC (version 0.11.5)