FastQCFastQC Report
Thu 26 May 2016
SRR1364262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4779196
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN98430.2059551439196049No Hit
GTATCAACGCAGAGTACTTTTTTTT50570.1058127768771149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34500.013.247051
TTACGCT1001.9247746E-611.4036224
TGACCGA4450.011.31535119
CGAGTCT855.3380914E-511.1743910
TTGACCG4900.010.47007718
GTATCAA81050.010.3748881
GGACCGA1951.4551915E-1110.2290796
ACATGGT18850.09.9818463
ACGGCCC3050.09.96849615
GTACTAG3350.09.9269641
GATCGTC1451.623539E-79.8259637
ACCGAGT2450.09.6923798
AACGACC1201.6997994E-59.50112316
TTGCGCG3100.09.50062518
CTCGAAC3300.09.50062518
AGGACCG2800.09.4984315
TACGCTG1701.672197E-89.4984315
AGCGCGT900.0011163029.49823210
ATCGTAC800.0045264599.49793212
TACATGT22350.09.4805642
Produced by FastQC (version 0.11.5)