FastQCFastQC Report
Thu 26 May 2016
SRR1364262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4779196
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT166540.3484686545603068No Hit
TATCAACGCAGAGTACTTTTTTTTT135030.28253706271933604No Hit
GGTATCAACGCAGAGTACTTTTTTT117670.24621296134328868No Hit
GTGGTATCAACGCAGAGTACATGGG66160.13843332644235556No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59720.12495825657704769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57150.012.9672091
TCGACCG603.809964E-412.77535618
CGCGCCA2150.011.44085810
TTCGCGG1354.143658E-911.35611316
TGGTATC19550.011.3280832
GCTTATA6600.011.1854041
CGGTTTG3100.011.12609914
GTGGTAT22050.010.8595761
TAGGTCG1106.3284133E-610.3249885
TTGACCG4550.010.31855718
ACGAAAC1701.3478711E-910.14535117
CGAAATC2250.010.09089613
GGTCGGT3100.010.07076311
ACGGCCC3450.09.99831715
CGAGTAT1252.306877E-69.96477818
CGGTTAC951.7156944E-49.9586429
ACCGAGT3100.09.7695748
TAGACAG8550.09.7413135
CGACCTA1106.231839E-59.58151718
TGACCGA4800.09.58141619
Produced by FastQC (version 0.11.5)