FastQCFastQC Report
Thu 26 May 2016
SRR1364261_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364261_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4167890
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN89940.215792643279933No Hit
GTATCAACGCAGAGTACTTTTTTTT58070.1393270935653292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA1900.013.4974696
TTGACCG4800.012.27222418
TGACCGA4450.012.16977219
GGTATCA27100.012.1671961
GCGCTTA658.045072E-411.68941313
GTATCAA71000.011.1217471
TCGCGGT1106.0428847E-610.36493617
ACATGGT16600.010.1899873
GTATAAG5600.010.1810731
CTACACG3000.010.1365574
AGGACCG3000.010.1313125
CGTGCGA750.00265408410.13106810
TGGTATC10000.010.0723542
ACAACCG3050.09.9652255
TTCGCGG1054.1019615E-59.95386916
GTATAGG5550.09.9303681
CAACCGT2400.09.8939786
GTGGTAT10600.09.8609141
TCAACGG2900.09.8307113
TAACGTA1106.818334E-59.5030214
Produced by FastQC (version 0.11.5)