FastQCFastQC Report
Thu 26 May 2016
SRR1364261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4167890
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT179500.43067355424447384No Hit
TATCAACGCAGAGTACTTTTTTTTT120340.28873122851130906No Hit
GGTATCAACGCAGAGTACTTTTTTT119140.2858520738311232No Hit
ACGCAGAGTACTTTTTTTTTTTTTT60970.14628505070911182No Hit
GTGGTATCAACGCAGAGTACATGGG56830.13635196706247046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA2650.014.6663956
AGGACCG3700.012.8101355
GGTATCA49500.012.139381
TGGTATC18450.012.0235022
GTGGTAT20900.011.5186051
ACCGAGT3200.011.25690658
AGCGCGT600.005992976511.0546410
CGCGTAT951.3945935E-510.9762637
CGAGTGG2850.010.97152210
TAACGCA1303.3778633E-810.9377544
GTCGGTT1951.8189894E-1210.68994512
ATAACGC1803.6379788E-1110.5326513
TTGACCG4400.010.41666318
CGAGCGA2550.010.404366510
CCGAGTG3600.010.2650229
CCGAGCG2500.010.2334389
CATTCCG2550.010.0327829
TATCACG1054.2046107E-59.93149952
TACGCGT1054.2073836E-59.9309045
ACGCGTA1151.0541813E-59.8916526
Produced by FastQC (version 0.11.5)