FastQCFastQC Report
Thu 26 May 2016
SRR1364260_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364260_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8998798
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN192370.2137729950155565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52100.012.4933431
GTATCAA129850.010.8450631
GCTTATA18050.010.476151
GTGGTAT19550.010.0611921
CGCGGTT5500.010.01981318
TTGACCG12000.09.97662518
CCTATAA19200.09.7991811
TTCGCGG5050.09.78393516
ACGGCCC6350.09.726047515
GTATTAG11950.09.5419951
TGACCGA11600.09.41963819
ACATGGT30350.09.2996623
CTTCGCG5650.09.24934615
TGGTATC20700.09.1351532
TAGACAG12550.09.0817535
ACAACCG7650.09.063455
TACATGT38500.08.9594012
TCGCGGT5500.08.63762517
GTATAAT13100.08.6318021
CAGTTGA18700.08.5363414
Produced by FastQC (version 0.11.5)