FastQCFastQC Report
Thu 26 May 2016
SRR1364260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8998798
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT186270.20699431190699022No Hit
TATCAACGCAGAGTACTTTTTTTTT127400.14157446361169568No Hit
GGTATCAACGCAGAGTACTTTTTTT120270.1336511831913551No Hit
CCTATAAGCAGTTCTTGTATTTTTA94820.10536962825479582No Hit
GTGGTATCAACGCAGAGTACATGGG94760.10530295268323614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71000.013.3667261
GTGGTAT24200.012.4975421
TGGTATC24000.012.0506462
CCTATAA21150.011.3412111
TATAAGC20950.010.9527113
CTATAAG21200.010.8243352
ACGGCCC8200.010.35841615
GCTTATA18600.010.2964551
ACCGGTC1951.6370905E-1110.2112168
ATAAGCA21850.010.0243394
GTATCAA150650.09.9812131
CGGTTTG3800.09.79387214
GTGCACG9500.09.77654111
TAAACGC3700.09.7381634
TTGACCG10500.09.63454718
CGCGCCA4650.09.57927110
AAGCAGT25600.09.5189356
TAAGCAG30100.09.4188935
ACGGGTT8500.09.31915115
GTATTTT24850.09.29407417
Produced by FastQC (version 0.11.5)