FastQCFastQC Report
Thu 26 May 2016
SRR1364259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3756226
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN72550.19314599281299902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27900.012.1595791
CGGATAG907.4843338E-611.6088765
TTGCGCG3900.011.4500318
AGGACCG2050.011.119815
GGACCGA2300.010.7372066
GTATCAA65150.010.0790171
TTTGCGC4450.010.03512517
ACATGGT16900.010.0100053
TACATGT20250.09.8080252
ACCGACC1554.0829036E-89.8043028
TGCGCGC4700.09.70323919
GTGTTAT4500.09.291691
GTGGTAT8850.09.1270461
ACCGTCC1252.7633847E-59.1180018
GTTCTAA5050.09.0324271
ATTTGCG4850.09.01158216
GTATTAG4750.09.0027141
GTTCGCG950.00182236479.00127116
GTATTGG4800.08.9089361
ACCGAGT2356.730261E-118.8916688
Produced by FastQC (version 0.11.5)