FastQCFastQC Report
Thu 26 May 2016
SRR1364259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3756226
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57880.15409083479002594No Hit
GTGGTATCAACGCAGAGTACATGGG44980.11974785329743205No Hit
TATCAACGCAGAGTACTTTTTTTTT44940.11964136343233873No Hit
GGTATCAACGCAGAGTACTTTTTTT41270.10987091831002713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40000.011.6851951
GTGGTAT13350.011.5048981
TATAACG1001.976925E-611.3781262
GTTTCGC700.001433811710.90925419
CCTACGC700.001518205310.8347243
TGTTCGA1002.4667275E-510.42439610
TGGTATC12900.010.2902962
TAGACCG750.00269487310.1124094
GGACCGA2750.09.6530116
AGGACCG3000.09.4806375
ACCGTGG2550.09.2947428
GTATCAA78250.09.2446231
TATAGCG850.00756437338.9229525
TAGACAG5250.08.8485945
CGTGCAC2800.08.79981410
CGTGCGC1752.728484E-78.664433510
AGACCCG1651.082306E-68.618765
ACCGAGT2651.0913936E-118.5862378
CGCGCCA2002.6497219E-88.52905110
CTGTTCG1451.7431925E-58.4964869
Produced by FastQC (version 0.11.5)