FastQCFastQC Report
Thu 26 May 2016
SRR1364258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068662
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN37690.1821950613488332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG551.9554293E-413.8225193
CGCCCGA655.4492815E-513.15510616
CTCAACG1450.013.1075614
CGACCGT801.9976142E-613.06406719
TCGACCG905.399979E-712.66818518
ACGAAAC1051.9921572E-812.66787917
CGCATAT1302.6229827E-911.690319
GCTTATA2500.011.4033031
TACATGT14550.011.3632892
GGTATCA23200.011.3049971
CGGGATT1354.7075446E-911.2627944
ACATGGT11050.011.0079793
CGATCTC700.001494191610.85502410
TTGCGCG7750.010.7883918
AACGGAG1803.6379788E-1110.5534955
TGCGCGC8100.010.43952419
AGTTCGA1106.04026E-610.36462815
TTTGCGC8150.010.2586517
ACATGTG13500.010.2069063
CATGTGA8100.010.0895854
Produced by FastQC (version 0.11.5)