FastQCFastQC Report
Thu 26 May 2016
SRR1364258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068662
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG29550.1428459555016721No Hit
GTATCAACGCAGAGTACTTTTTTTT27950.13511148752188612No Hit
TATCAACGCAGAGTACTTTTTTTTT24980.12075438133440843No Hit
GGTCGGGAGTGGGTAATTTGCGCGC24230.11712884946888376No Hit
GGTATCAACGCAGAGTACATGGGAT23810.11509855162418993No Hit
GGTATCAACGCAGAGTACTTTTTTT22830.11036118998657103No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC250.006099416418.9638713
TATTGCG400.00533839614.2263452
CGCCAAT400.005361565414.2170612
GATCGAC551.9995097E-413.7862411
CGACATG603.943745E-412.72257315
CGCGCCA1800.012.63799810
ATTGCGC550.0031130912.0679193
ACGGCCC3150.011.51089915
TGCACGG3050.011.4979512
GGTACAT3500.011.3785981
GGGTATC3350.011.3219881
GGTATCA32500.011.2618951
CGTCTTA600.005687216811.13225215
TAACGCC600.005958460711.0627954
AATACCG600.005958460711.0627955
GCCCCCG3650.010.97975418
CGGTTTG1751.8189894E-1110.90373514
TCTAATA1408.574716E-910.8391192
CGGGACC700.001515023810.8370224
CGTCCGA700.0015208410.83204611
Produced by FastQC (version 0.11.5)