FastQCFastQC Report
Thu 26 May 2016
SRR1364257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714351
Sequences flagged as poor quality0
Sequence length25
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA224581.3100001108291126No Hit
GTATCAACGCAGAGTACTTTTTTTT103590.6042519880701209No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA74970.4373083458404959No Hit
TATCAACGCAGAGTACTTTTTTTTT74270.433225168008185No Hit
GGTATCAACGCAGAGTACTTTTTTT63570.3708108782857186No Hit
GAACAAAAAAAAAAAAAAAAAAAAA62370.3638111448589No Hit
AAACAAAAAAAAAAAAAAAAAAAAA56710.33079573552907193No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41750.2435323921413993No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN34580.20170898491615774No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25480.14862767309611627No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23850.13911970185802092No Hit
ACACAAAAAAAAAAAAAAAAAAAAA21800.12716182392053904No Hit
GAAAACAAAAAAAAAAAAAAAAAAA21470.1252368972281639No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA18600.10849586811568926No Hit
GTATCAACTCAGAGTACTTTTTTTT17800.10382937916447682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT2850.015.33437219
GTTCGAC3100.014.09896716
TCGACCG3100.014.0977318
TTCGACC3250.014.03254117
AGTTCGA3200.013.65797315
GCGTAGG707.276043E-613.56917311
GAACAAA17350.013.5277871
GGTATCA31750.013.4678421
CCTCTAG3950.013.2310671
TAGATAG4050.013.132855
GCCGAAT604.088041E-412.66826515
CTCAACG2550.012.2974694
TGGTCCG550.003073830612.0881925
GTACAAG6150.012.0517121
GGCGTAG802.8724955E-511.87267810
AAGTTCG3600.011.61291714
GATAGTC3800.011.4974157
TAGTCAA3650.011.4498169
GTACTAG752.0693644E-411.4027741
AGTCAAG4000.011.39777110
Produced by FastQC (version 0.11.5)