##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364257_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1714351 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.70814436483544 28.0 15.0 31.0 2.0 33.0 2 23.411336418271404 28.0 16.0 31.0 2.0 34.0 3 23.15776115859588 28.0 16.0 31.0 2.0 34.0 4 25.739863656859068 32.0 17.0 35.0 2.0 37.0 5 26.681403633211634 32.0 20.0 35.0 2.0 37.0 6 26.846280020835874 33.0 22.0 35.0 2.0 37.0 7 26.873913801782717 33.0 23.0 35.0 2.0 37.0 8 25.196582263492132 32.0 13.0 35.0 2.0 37.0 9 24.859445352789482 31.0 10.0 37.0 2.0 39.0 10 26.58970829194255 32.0 17.0 37.0 2.0 39.0 11 26.873879969737818 33.0 17.0 37.0 2.0 39.0 12 25.776266353856357 32.0 11.0 37.0 2.0 39.0 13 25.448040687117167 32.0 11.0 37.0 2.0 39.0 14 25.86258414992029 32.0 11.0 38.0 2.0 40.0 15 25.461967823392058 32.0 10.0 38.0 2.0 40.0 16 26.18457072093171 32.0 15.0 38.0 2.0 40.0 17 26.687785640163536 33.0 17.0 38.0 2.0 40.0 18 27.124560256330238 33.0 17.0 39.0 2.0 40.0 19 27.671467511612267 34.0 18.0 39.0 2.0 41.0 20 27.64697019455176 34.0 18.0 40.0 2.0 41.0 21 27.785136182730373 34.0 18.0 40.0 2.0 41.0 22 27.68671584757147 34.0 18.0 40.0 2.0 41.0 23 27.319502832267137 34.0 15.0 40.0 2.0 41.0 24 27.263635043232103 34.0 10.0 40.0 2.0 41.0 25 26.98886459074017 34.0 2.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.7097632108745984 1101 2 -0.7702062989271603 1101 3 -2.8946937726801103 1101 4 -2.843227309564128 1101 5 -3.5009307862993317 1101 6 -1.9902842662754772 1101 7 -1.47695480465325 1101 8 -4.853165516391545 1101 9 -5.995416471593149 1101 10 -5.718045083148105 1101 11 -3.1758700154641737 1101 12 -0.8644403859752252 1101 13 -0.3175487530867578 1101 14 -6.710983077757344 1101 15 -8.393262093973586 1101 16 -4.071659245806952 1101 17 -2.3735491898004177 1101 18 -6.390312910638041 1101 19 -3.512292132136018 1101 20 -2.4685086785862858 1101 21 -2.337628021369504 1101 22 -2.227806611402471 1101 23 -6.42568692937709 1101 24 -3.7327832044159663 1101 25 -2.815369447419396 1102 1 1.4803051403338294 1102 2 0.9751623896284727 1102 3 1.499333918072221 1102 4 1.4005359118700582 1102 5 1.056890613915055 1102 6 0.511004861652335 1102 7 0.5176456896241994 1102 8 1.6349758272921946 1102 9 -3.202995629533568 1102 10 -0.5424234979657037 1102 11 -0.02555983238972459 1102 12 -3.8164612590795244 1102 13 -5.594121448524241 1102 14 -5.157298975660851 1102 15 -0.8141764828761566 1102 16 0.4189852815859503 1102 17 -3.8659924011247533 1102 18 -1.5141418257876218 1102 19 -0.6985877778296192 1102 20 -0.39317769067341146 1102 21 -0.21754925961497307 1102 22 -0.13189031268074913 1102 23 0.07840140422849373 1102 24 0.09514005823780636 1102 25 0.2254070200328151 1103 1 -0.8660697175063383 1103 2 0.854781076167729 1103 3 1.7191645449331787 1103 4 2.2773885903228184 1103 5 1.9772721114014686 1103 6 1.2746799115744096 1103 7 1.4839405699073218 1103 8 2.77958861887576 1103 9 3.8095030873985536 1103 10 2.3306947884199545 1103 11 1.9973897024547185 1103 12 3.062513542080737 1103 13 3.2874945299750173 1103 14 -2.034761801452742 1103 15 1.58729392313764 1103 16 2.088198931710618 1103 17 2.1190689060055057 1103 18 1.8519512805698106 1103 19 1.4576715895882693 1103 20 1.5348210383226153 1103 21 1.3308032812274782 1103 22 1.3431644962985594 1103 23 1.7210433831015273 1103 24 1.3882537971446354 1103 25 1.4708371514148766 1104 1 1.7495594783257928 1104 2 1.2060363757566286 1104 3 1.9246888799813355 1104 4 2.192582094059251 1104 5 1.3608268359473605 1104 6 0.8124246057598583 1104 7 0.7593886614327516 1104 8 1.9270080848390663 1104 9 -2.89532646454105 1104 10 -0.5167883867471517 1104 11 0.3097464864473167 1104 12 1.6887109949577166 1104 13 1.7519189577854348 1104 14 1.5763005650847752 1104 15 -3.163891545353284 1104 16 -3.9915876113283737 1104 17 -0.9909171485270818 1104 18 -0.336922851210705 1104 19 -0.20555253191991696 1104 20 0.11074124202605162 1104 21 -0.011184196904906685 1104 22 -0.11835587093208133 1104 23 -0.2599536348096798 1104 24 -5.713398180434705 1104 25 -3.3248920491902574 1105 1 -2.353634585292287 1105 2 -0.13599167751162966 1105 3 0.8414229855945692 1105 4 1.3026360329341422 1105 5 1.168667791276583 1105 6 0.9125027742279066 1105 7 0.29162939083667894 1105 8 2.238374832867617 1105 9 2.46783402611479 1105 10 1.396718124396724 1105 11 1.4227353486249896 1105 12 1.887788659673415 1105 13 2.132680039679144 1105 14 2.5163389808073227 1105 15 2.9096236617036695 1105 16 2.5509585120156792 1105 17 1.1661719945833298 1105 18 1.4286082758943799 1105 19 0.6035057459629343 1105 20 0.758612664634267 1105 21 0.7012914379572806 1105 22 0.5814073075610082 1105 23 1.051254565457409 1105 24 1.172425849447233 1105 25 1.2558730332445762 1106 1 2.296192390095129 1106 2 1.6151646613821917 1106 3 2.108694758906111 1106 4 2.2506898630030427 1106 5 1.8533172752502267 1106 6 1.3122717834931095 1106 7 1.2427362454158732 1106 8 2.8418065338578984 1106 9 3.5863190175775763 1106 10 2.0407029884192838 1106 11 1.0868150639772303 1106 12 2.3085450280617614 1106 13 -3.148865997782316 1106 14 0.8180435897107508 1106 15 2.594150500444478 1106 16 2.040645656919903 1106 17 1.8420370537130353 1106 18 1.4069311838501477 1106 19 1.3951881828246115 1106 20 0.8855365218705273 1106 21 0.6354891271548695 1106 22 0.7412307751455849 1106 23 1.0957862929586675 1106 24 1.0537568368575023 1106 25 1.2836217569243331 1107 1 1.6261764343557843 1107 2 1.0175243770455928 1107 3 1.706183124691652 1107 4 1.5841296251518067 1107 5 1.3783351278257179 1107 6 0.5860129194318908 1107 7 0.1102589774092344 1107 8 1.560475049296084 1107 9 2.815847563674126 1107 10 1.4178386456772998 1107 11 0.24238781408065435 1107 12 1.759123219767524 1107 13 2.206835625600437 1107 14 -2.8974724012187707 1107 15 0.9834563785766157 1107 16 1.0704620328814087 1107 17 0.7749587907686326 1107 18 0.8687071460438851 1107 19 0.4899696947660992 1107 20 0.6436328365153017 1107 21 0.15892900088222817 1107 22 0.1648582296601404 1107 23 0.557229235955738 1107 24 0.618365994198772 1107 25 0.6714423522733952 1108 1 1.2562999671085286 1108 2 0.6945946586193799 1108 3 0.8989264898081721 1108 4 1.1300559702001713 1108 5 0.6481844875723723 1108 6 0.0930348377823762 1108 7 -0.16124652154088892 1108 8 -3.833111445144727 1108 9 -5.0208910597811744 1108 10 -2.155555127342563 1108 11 -0.7518669017638935 1108 12 0.11213236747328281 1108 13 1.2411378010849319 1108 14 1.4527272476745559 1108 15 -3.198217229905019 1108 16 -0.33558552141964526 1108 17 0.13049108741934745 1108 18 -0.029347998357341254 1108 19 -0.027204412837772196 1108 20 -0.11097888080940166 1108 21 -0.5317948554394114 1108 22 -0.5240199500927076 1108 23 -0.28640910695844823 1108 24 -0.1742891639713413 1108 25 0.10021244624606496 1109 1 0.9170740504531381 1109 2 0.5509816495542985 1109 3 0.9167167076406706 1109 4 -0.8628010518536797 1109 5 -1.9115190369845223 1109 6 -0.5213263785618274 1109 7 -0.519840588649533 1109 8 1.2942503800480765 1109 9 -3.3473734369967083 1109 10 -0.6652747187374999 1109 11 0.052992893933577534 1109 12 -5.901044976593827 1109 13 -1.2288442717063006 1109 14 0.3962869824871653 1109 15 1.6646463880086593 1109 16 1.380280058339519 1109 17 1.0305967903012316 1109 18 0.8061309625410509 1109 19 0.23181669391368231 1109 20 0.13240496440775473 1109 21 0.10057474948549583 1109 22 -0.10327512530580307 1109 23 0.10188916120553415 1109 24 0.06277956827057452 1109 25 0.17421122854886661 1110 1 0.47154078257617016 1110 2 0.4350974766946685 1110 3 -2.3769170760679508 1110 4 -2.076939808875931 1110 5 -0.3519148464871442 1110 6 -0.2862808815488762 1110 7 -0.4639832109832156 1110 8 -3.6797973184592436 1110 9 -6.594594765777117 1110 10 -2.877058114584891 1110 11 -1.1206309302499733 1110 12 1.0147351673940648 1110 13 1.3020501065428363 1110 14 1.4365837610181238 1110 15 1.977597660095146 1110 16 1.5016731060992932 1110 17 0.8905265093466994 1110 18 0.6198391746551515 1110 19 -0.0815536561247292 1110 20 0.007532531776625717 1110 21 -0.15627136597728253 1110 22 -0.5272283473794346 1110 23 -0.11998525688170503 1110 24 -0.08399646373533187 1110 25 0.1547634521710357 1111 1 0.9673376455942737 1111 2 0.9079131491635621 1111 3 0.9803352242346186 1111 4 1.6309042428386995 1111 5 -1.3526207664854404 1111 6 -0.09931726516439454 1111 7 -0.426812939747677 1111 8 1.5038168006899184 1111 9 2.621888747131578 1111 10 1.3757030420513736 1111 11 1.171336141800694 1111 12 2.3289618360283555 1111 13 2.365935324207417 1111 14 2.628468904551738 1111 15 2.919210258968061 1111 16 2.207094494795051 1111 17 1.7522958202905095 1111 18 1.019215015590607 1111 19 0.6855361189380673 1111 20 0.7172367580992507 1111 21 0.5089668009414012 1111 22 0.5254249447599548 1111 23 0.7345887172941055 1111 24 0.6291874819030099 1111 25 0.767905492127035 1112 1 2.004101390309387 1112 2 1.4209238745162622 1112 3 1.9730604855117164 1112 4 2.0376788219724595 1112 5 1.6880240418151224 1112 6 1.0154871739800129 1112 7 0.9178586073527732 1112 8 -2.9533920573896246 1112 9 1.6679851485969124 1112 10 1.111133916696108 1112 11 1.1147248007065684 1112 12 2.150075439438911 1112 13 2.4271139020516337 1112 14 2.5062382573415825 1112 15 3.194525788354916 1112 16 2.2447816912548966 1112 17 1.7400272044977605 1112 18 1.3585178716748842 1112 19 1.0813515753469751 1112 20 0.9332007647330265 1112 21 0.8044866966551076 1112 22 0.60857269222738 1112 23 0.9923469349319909 1112 24 1.0221427640630303 1112 25 1.004670018748751 1113 1 -0.06355013694072298 1113 2 0.08823193859856815 1113 3 -5.5035804468084955 1113 4 -3.146857560537711 1113 5 -0.9782662407723777 1113 6 -1.0150858810245253 1113 7 -0.7024202187575703 1113 8 0.9473766108684529 1113 9 -5.496503974894331 1113 10 -1.9778357568405234 1113 11 -0.6215750219407781 1113 12 -4.370706551281593 1113 13 -0.2815698638764843 1113 14 0.7595468089335533 1113 15 1.9140692523632552 1113 16 1.3271919432469623 1113 17 1.0601467494327004 1113 18 0.5145607851335114 1113 19 0.3759148969688688 1113 20 0.16806169839809115 1113 21 -0.06084789887997388 1113 22 -0.1902646392455729 1113 23 0.05923924993047791 1113 24 0.21041342858913836 1113 25 0.08130149506250461 1114 1 0.6136100682347063 1114 2 0.5534906020806503 1114 3 1.2846108152505984 1114 4 1.6019403071342069 1114 5 1.5275757194730843 1114 6 0.7063722889430295 1114 7 0.9532400907321517 1114 8 -2.7286627551883953 1114 9 -4.6007074812934725 1114 10 -1.5809946814680487 1114 11 -1.4198239805963446 1114 12 1.2488495014654681 1114 13 2.034515760174795 1114 14 2.337489371324292 1114 15 2.8740190376196892 1114 16 2.0084934259487746 1114 17 1.9041767343970548 1114 18 1.7983697676734565 1114 19 0.8688941851856704 1114 20 1.3670663961384264 1114 21 1.2132278404925572 1114 22 1.1107421735570284 1114 23 1.1209771260914252 1114 24 1.2581803832591802 1114 25 1.2608192033134067 1115 1 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13 2.7865812136386268 1116 14 2.718479635102316 1116 15 3.722807586981588 1116 16 3.111400061298159 1116 17 2.486110234744501 1116 18 2.3006980504110857 1116 19 1.689826677807222 1116 20 1.6860225408304288 1116 21 1.3126215382311983 1116 22 1.5325427720385179 1116 23 1.424944309612929 1116 24 1.8386593479426168 1116 25 1.8828591687481513 1201 1 2.7433248209096988 1201 2 2.1162199022840973 1201 3 2.61141024549368 1201 4 3.023911558244354 1201 5 2.4831138248043416 1201 6 1.7714339605893947 1201 7 1.5031824908047895 1201 8 2.635524844653844 1201 9 3.8298846062357192 1201 10 2.49744194868083 1201 11 2.19702442377217 1201 12 3.1891832121776353 1201 13 3.364521491793184 1201 14 3.2383997622944385 1201 15 -0.6922393221727248 1201 16 1.3637565838435108 1201 17 1.8565347846253282 1201 18 -4.828178407714066 1201 19 -1.2397937565674653 1201 20 0.20287751945050658 1201 21 0.6874830847815083 1201 22 0.9716915508819532 1201 23 1.3606587942761195 1201 24 1.3532124604601066 1201 25 1.4593417509894024 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2.017964449421303 2204 3 -0.7948203971252497 2204 4 1.8354965728898591 2204 5 1.8917892111320604 2204 6 1.8172638349598635 2204 7 1.7955410012882673 2204 8 3.207799438136032 2204 9 -2.220697011964475 2204 10 0.6132664731887338 2204 11 1.7272081023868608 2204 12 3.2524538434819164 2204 13 3.5682235556922706 2204 14 3.6943261186690783 2204 15 4.109187717795461 2204 16 3.685622763488208 2204 17 2.830450617553833 2204 18 2.7593453438309368 2204 19 2.20776570172308 2204 20 2.17479648030632 2204 21 1.9302163931078233 2204 22 1.855276081785096 2204 23 2.3063000911156024 2204 24 2.190416962543683 2204 25 2.172535208988883 2205 1 -2.3967342198463832 2205 2 -2.6351728684035542 2205 3 0.14533311337283905 2205 4 -1.4849663805423283 2205 5 -2.134483164042269 2205 6 -0.45734335698477224 2205 7 -0.2546088561201394 2205 8 -3.350909749687272 2205 9 1.3371848822331671 2205 10 0.8236814117017879 2205 11 0.8084348543057445 2205 12 2.067077100948044 2205 13 -3.572238550061652 2205 14 0.22438600108399243 2205 15 2.293216248224084 2205 16 1.9853008475738534 2205 17 1.950553509549529 2205 18 0.9431003971034819 2205 19 0.8510316042683321 2205 20 0.801792611260506 2205 21 0.8105682199554067 2205 22 0.9154251116980454 2205 23 1.0115001936443058 2205 24 0.5759204531617037 2205 25 1.0677714589409497 2206 1 -6.542366489984705 2206 2 -5.1946952719047665 2206 3 -2.280772402920384 2206 4 -1.0364387784021716 2206 5 -0.8196335137303024 2206 6 -1.2960456700788896 2206 7 -1.5656153361091079 2206 8 -2.738003590211761 2206 9 -5.51752638843454 2206 10 -2.567273523970087 2206 11 -1.8744453108753625 2206 12 -7.001962614524508 2206 13 -5.96654366840178 2206 14 -6.879149324126207 2206 15 -2.2752594401056676 2206 16 -5.711771269822968 2206 17 -2.7636205398354505 2206 18 -2.077132550835131 2206 19 -2.03482278413556 2206 20 -2.0595283009393057 2206 21 -2.083637707025236 2206 22 -2.0600044627666243 2206 23 -1.730506599314296 2206 24 -1.5978037557102276 2206 25 -1.9562131657385216 2207 1 -6.279620961625739 2207 2 -4.359116272427638 2207 3 -0.75845902907378 2207 4 0.05586605213759199 2207 5 0.3998217905756434 2207 6 0.2899194398242031 2207 7 0.3458769545221507 2207 8 1.4638086741270868 2207 9 -1.424165459292194 2207 10 -0.37038439684399904 2207 11 -0.12152383010168677 2207 12 -3.667795235196291 2207 13 -3.969032103431541 2207 14 -0.7535386524807279 2207 15 -4.262640529207889 2207 16 -0.6363590536494677 2207 17 0.18462037839632117 2207 18 0.27878627329165084 2207 19 0.2806300273115667 2207 20 0.10831358292998416 2207 21 0.07377288335201015 2207 22 0.08468590116443764 2207 23 -0.10789331418083492 2207 24 -0.11738808358676422 2207 25 0.2421855522691274 2208 1 -3.4001568529910777 2208 2 -3.663919607831094 2208 3 -1.1281212359980124 2208 4 0.00154240693803942 2208 5 -2.355135698896632 2208 6 -0.6808953852524198 2208 7 -0.2721445964594018 2208 8 -4.586406399561575 2208 9 -7.155539734477976 2208 10 -3.119738642953344 2208 11 -1.055007775206537 2208 12 0.31324350845840954 2208 13 1.1160139158452687 2208 14 1.081471105117597 2208 15 1.5011027456074792 2208 16 -4.018620689109383 2208 17 -5.983636276534391 2208 18 -2.2357362575939277 2208 19 -1.5546399263307151 2208 20 -0.9014366150538464 2208 21 -0.744098272934739 2208 22 -0.4390516349319675 2208 23 -0.3091082333503472 2208 24 -0.29347834645285076 2208 25 -0.0189539860244885 2209 1 0.763706148983335 2209 2 0.5907115225247033 2209 3 -1.9339565684291387 2209 4 0.20037479611110243 2209 5 0.5398401244456181 2209 6 0.15251981131087078 2209 7 0.14146486120591817 2209 8 1.5783121767799067 2209 9 -3.550136232624528 2209 10 -0.6564388084387929 2209 11 -0.0495642701849448 2209 12 -4.141922054825066 2209 13 -0.10564279685963385 2209 14 1.0403479265907833 2209 15 1.805457637737458 2209 16 -3.001705888906411 2209 17 -0.5064035715653894 2209 18 -0.043082037597454104 2209 19 -0.2020954125263401 2209 20 -0.10094306642136175 2209 21 -0.0497896389959358 2209 22 -0.14233200241285715 2209 23 0.33506006152488865 2209 24 -0.22709981485891717 2209 25 -0.07471748212515905 2210 1 -0.3676930099660609 2210 2 -2.888633061755179 2210 3 -1.1091175851030322 2210 4 -2.8352713627026382 2210 5 -3.4060393580847155 2210 6 -1.7602041838514637 2210 7 -0.9341497343274554 2210 8 -4.4927356782735615 2210 9 -4.20759391738531 2210 10 -1.845323451527257 2210 11 -1.1184858071562083 2210 12 0.5517977694752219 2210 13 -6.413653125618332 2210 14 -1.5842924488059076 2210 15 -4.932492334845286 2210 16 -1.5681936906156437 2210 17 -0.8076123191030931 2210 18 -0.5727771233169783 2210 19 -0.7166377354079998 2210 20 -0.6321687419653585 2210 21 -0.7570269348711207 2210 22 -0.7128801373707176 2210 23 -0.4507899593962925 2210 24 -0.3964315598383088 2210 25 -0.38679110557828267 2211 1 0.9416620749224904 2211 2 0.648526615935932 2211 3 -2.48081694187281 2211 4 -2.1178412111619593 2211 5 -0.1618130679076053 2211 6 -0.2915822228417184 2211 7 -0.362834928138966 2211 8 -3.841703933028409 2211 9 0.9430304817544091 2211 10 0.5571553139464456 2211 11 -4.8225597637654225 2211 12 0.17693925039140268 2211 13 1.2319602226477215 2211 14 1.7011372468242882 2211 15 -3.260150738960373 2211 16 -0.24150657405122544 2211 17 -4.666133880695607 2211 18 -1.1147744076762756 2211 19 -0.5513201342900302 2211 20 -0.012681542147724656 2211 21 0.06123773107534092 2211 22 0.2952301136773805 2211 23 0.36287347955630267 2211 24 0.4437088804126397 2211 25 0.6153472136978131 2212 1 2.0569649674429087 2212 2 1.2724582144275765 2212 3 1.9581846654251045 2212 4 2.2677127113223534 2212 5 -0.8255118442673925 2212 6 0.5911802768247085 2212 7 1.3284557957198615 2212 8 -1.2563881287533682 2212 9 2.589807880230861 2212 10 1.9072240808430863 2212 11 1.732118335312279 2212 12 3.158507258799439 2212 13 3.137778107377983 2212 14 3.357599179728325 2212 15 3.8117406380813392 2212 16 3.092991519976728 2212 17 2.327344143662245 2212 18 2.2424168648808056 2212 19 1.848624852192657 2212 20 1.6433463382101934 2212 21 1.3959056896017152 2212 22 1.4548672297374274 2212 23 1.5919505867484993 2212 24 1.6038673932412593 2212 25 1.7663262695591122 2213 1 2.2906289576529772 2213 2 1.914025760471631 2213 3 2.3948781974201054 2213 4 2.5783410723114777 2213 5 1.4936863549356119 2213 6 -0.37891936324521325 2213 7 0.8588333001421127 2213 8 -5.089279410690256 2213 9 0.6417759774797354 2213 10 0.797006830258578 2213 11 0.907885753561871 2213 12 2.3963404651374596 2213 13 -1.8034473852196342 2213 14 0.8508253436116924 2213 15 -3.369893755098232 2213 16 0.15102829059192402 2213 17 1.1310628999490326 2213 18 1.368471612194881 2213 19 1.2198995550392624 2213 20 1.267090934828957 2213 21 1.1037306507995233 2213 22 1.14294607037273 2213 23 1.510616604366625 2213 24 1.5779311633029565 2213 25 1.828626590713057 2214 1 2.4126391323292715 2214 2 1.4930294475146937 2214 3 1.1180729135598568 2214 4 2.010708322234102 2214 5 1.8114689005278386 2214 6 0.8519814832421808 2214 7 1.024227982025721 2214 8 2.467781411998132 2214 9 3.107650245404866 2214 10 1.5475415566751742 2214 11 1.8027202873627708 2214 12 2.7418761982039577 2214 13 1.3027124106858565 2214 14 2.709224259434727 2214 15 3.610188399553241 2214 16 2.638346905802255 2214 17 1.8119218277316698 2214 18 1.3498885545634245 2214 19 1.3716822268383417 2214 20 0.9985690274227181 2214 21 0.6583475679266932 2214 22 1.0220375012279028 2214 23 -5.046610090185748 2214 24 -1.739312298731317 2214 25 -0.19446655895925602 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 246503.0 3 6.0 4 1464.0 5 11666.0 6 18819.0 7 14999.0 8 11025.0 9 9437.0 10 9222.0 11 8849.0 12 8335.0 13 8490.0 14 8531.0 15 7561.0 16 6753.0 17 6812.0 18 8845.0 19 13256.0 20 19002.0 21 24995.0 22 29607.0 23 33625.0 24 37891.0 25 41410.0 26 44167.0 27 47524.0 28 50045.0 29 55735.0 30 59638.0 31 65336.0 32 79336.0 33 97229.0 34 113551.0 35 126991.0 36 140930.0 37 131989.0 38 114777.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.96075991992966 19.913171384206684 14.477262735487251 20.648805960376407 2 14.704668265593334 25.580975189351197 37.40856942058593 22.305787124469543 3 15.18747233509083 32.90400707753893 28.905057247723803 23.003463339646437 4 9.589538833843738 20.795716112906078 34.00803462871146 35.60671042453873 5 10.451202653486561 43.07155571877346 31.17425668927027 15.302984938469708 6 27.684193471215103 40.43263187510273 16.604421193136055 15.278753460546119 7 25.663090487738398 39.27204813160151 18.40860622608423 16.65625515457586 8 23.680665672712088 36.731846924755615 20.74711537730403 18.840372025228273 9 24.06012618613918 21.826715755952776 20.796554390718427 33.31660366718962 10 15.097096464752813 36.03319997192391 26.998022975597202 21.871680587726072 11 30.27355939559453 29.89494322512191 20.05566061298034 19.77583676630322 12 21.305119920263728 33.72946054013837 26.97890077186021 17.986518767737696 13 25.48880259376698 28.361166727205834 24.8904299567686 21.259600722258583 14 20.647546899656902 28.625606387968983 26.22257289815724 24.50427381421688 15 20.858703014181287 33.90787252634645 24.936451508377406 20.296972951094858 16 20.995788734865766 34.501900918289756 23.81757033397672 20.684740012867756 17 20.143728783423054 33.89616481493179 24.73656936577788 21.223537035867274 18 19.45442337139428 31.512989891876654 27.935490965155573 21.09709577157349 19 21.250596535881048 31.519435372940197 27.959227357369443 19.270740733809312 20 22.16934681217449 30.152558528163908 27.67498181737329 20.003112842288314 21 23.40098494507937 29.808333430814045 27.083357703510476 19.707323920596114 22 22.90640653404239 29.98271033613072 26.927774125398024 20.183109004428868 23 21.86501063679823 30.455402364603824 27.819453437613145 19.860133560984796 24 21.94751160653651 30.4103832657638 27.418040625948688 20.224064501750995 25 21.06035862352952 30.183932598991138 28.251137076170064 20.504571701309278 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 29135.0 1 29135.0 2 29370.5 3 29606.0 4 29606.0 5 29606.0 6 33233.5 7 36861.0 8 36861.0 9 36861.0 10 32927.0 11 28993.0 12 28993.0 13 28993.0 14 26685.5 15 24378.0 16 24378.0 17 24378.0 18 28833.0 19 33288.0 20 33288.0 21 33288.0 22 43806.0 23 54324.0 24 54324.0 25 54324.0 26 70823.5 27 87323.0 28 87323.0 29 87323.0 30 100094.0 31 112865.0 32 112865.0 33 112865.0 34 123225.0 35 133585.0 36 133585.0 37 133585.0 38 144449.0 39 155313.0 40 155313.0 41 155313.0 42 168823.5 43 182334.0 44 182334.0 45 182334.0 46 190705.5 47 199077.0 48 199077.0 49 199077.0 50 191100.0 51 183123.0 52 183123.0 53 183123.0 54 166373.0 55 149623.0 56 149623.0 57 149623.0 58 130905.0 59 112187.0 60 112187.0 61 112187.0 62 93697.5 63 75208.0 64 75208.0 65 75208.0 66 59192.0 67 43176.0 68 43176.0 69 43176.0 70 33318.5 71 23461.0 72 23461.0 73 23461.0 74 17211.5 75 10962.0 76 10962.0 77 10962.0 78 8263.5 79 5565.0 80 5565.0 81 5565.0 82 4048.0 83 2531.0 84 2531.0 85 2531.0 86 1774.0 87 1017.0 88 1017.0 89 1017.0 90 684.0 91 351.0 92 351.0 93 351.0 94 201.0 95 51.0 96 51.0 97 51.0 98 32.5 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22486643633654954 2 0.23548269870055782 3 0.2421907765679257 4 0.3061800063114263 5 0.25076545001577855 6 0.27543951034531433 7 0.2744478814431817 8 0.27310626586970815 9 0.2807476415273185 10 0.27479786811452267 11 0.27438955033129153 12 0.27503119256208325 13 0.2749728614501931 14 0.27048136583465116 15 0.2702480413870905 16 0.2712980014011133 17 0.2684981080303859 18 0.27048136583465116 19 0.26126505015600654 20 0.30927155524160455 21 0.2689647569255071 22 0.27176465029623453 23 0.27532284812153407 24 0.27730610592579935 25 0.2743312192194014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1714351.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.48100680465195 #Duplication Level Percentage of deduplicated Percentage of total 1 89.60935952844483 58.677110810425994 2 5.394591125483624 7.064865163922164 3 1.8362480187374353 3.6071810702992395 4 0.913271382102793 2.3920771834386754 5 0.5358416153784142 1.7543724231404823 6 0.3438861591611693 1.3510807156834894 7 0.24121739752470203 1.1056610634100814 8 0.1863659851693215 0.9762745874422406 9 0.12530980220620108 0.738487080985846 >10 0.7160847346816643 8.522164350723886 >50 0.05692204081601732 2.5546001397012814 >100 0.03626879303475241 4.469048909194118 >500 0.0024058128076215614 1.045099156642153 >1k 0.0016038752050810407 1.8907706157964277 >5k 4.4552089030028914E-4 1.9364557787961378 >10k+ 1.7820835612011567E-4 1.9147509503978082 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAA 22458 1.3100001108291126 No Hit GTATCAACGCAGAGTACTTTTTTTT 10359 0.6042519880701209 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 7497 0.4373083458404959 No Hit TATCAACGCAGAGTACTTTTTTTTT 7427 0.433225168008185 No Hit GGTATCAACGCAGAGTACTTTTTTT 6357 0.3708108782857186 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 6237 0.3638111448589 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 5671 0.33079573552907193 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 4175 0.2435323921413993 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 3458 0.20170898491615774 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2548 0.14862767309611627 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2385 0.13911970185802092 No Hit ACACAAAAAAAAAAAAAAAAAAAAA 2180 0.12716182392053904 No Hit GAAAACAAAAAAAAAAAAAAAAAAA 2147 0.1252368972281639 No Hit ACAAAAAAAAAAAAAAAAAAAAAAA 1860 0.10849586811568926 No Hit GTATCAACTCAGAGTACTTTTTTTT 1780 0.10382937916447682 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.8331111890155515E-5 0.0 0.0 0.0 0.0 6 5.8331111890155515E-5 0.0 0.0 0.0 0.0 7 5.8331111890155515E-5 0.0 0.0 0.0 0.0 8 5.8331111890155515E-5 0.0 0.0 0.0 0.0 9 5.8331111890155515E-5 0.0 0.0 5.8331111890155515E-5 0.0 10 5.8331111890155515E-5 0.0 0.0 5.8331111890155515E-5 0.0 11 5.8331111890155515E-5 0.0 0.0 5.8331111890155515E-5 0.0 12 5.8331111890155515E-5 0.0 0.0 5.8331111890155515E-5 0.0 13 5.8331111890155515E-5 0.0 0.0 5.8331111890155515E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACCGT 285 0.0 15.334372 19 GTTCGAC 310 0.0 14.098967 16 TCGACCG 310 0.0 14.09773 18 TTCGACC 325 0.0 14.032541 17 AGTTCGA 320 0.0 13.657973 15 GCGTAGG 70 7.276043E-6 13.569173 11 GAACAAA 1735 0.0 13.527787 1 GGTATCA 3175 0.0 13.467842 1 CCTCTAG 395 0.0 13.231067 1 TAGATAG 405 0.0 13.13285 5 GCCGAAT 60 4.088041E-4 12.668265 15 CTCAACG 255 0.0 12.297469 4 TGGTCCG 55 0.0030738306 12.088192 5 GTACAAG 615 0.0 12.051712 1 GGCGTAG 80 2.8724955E-5 11.872678 10 AAGTTCG 360 0.0 11.612917 14 GATAGTC 380 0.0 11.497415 7 TAGTCAA 365 0.0 11.449816 9 GTACTAG 75 2.0693644E-4 11.402774 1 AGTCAAG 400 0.0 11.397771 10 >>END_MODULE