FastQCFastQC Report
Thu 26 May 2016
SRR1364257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714351
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT421432.458248048386824No Hit
TATCAACGCAGAGTACTTTTTTTTT385962.2513475945124424No Hit
GGTATCAACGCAGAGTACTTTTTTT310781.8128142953222532No Hit
GTACTTTTTTTTTTTTTTTTTTTTT163340.9527803816138002No Hit
ACGCAGAGTACTTTTTTTTTTTTTT154280.8999323942413193No Hit
GAGTACTTTTTTTTTTTTTTTTTTT113390.6614164777224734No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA78620.45859920168040263No Hit
ATCAACGCAGAGTACTTTTTTTTTT60960.35558645808238803No Hit
GCAGAGTACTTTTTTTTTTTTTTTT58890.34351191792112584No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT58540.34147032900497043No Hit
GTGGTATCAACGCAGAGTACTTTTT51590.3009302062413123No Hit
GTGGTATCAACGCAGAGTACATGGG26740.15597739319427587No Hit
CAACGCAGAGTACTTTTTTTTTTTT26560.15492743318025307No Hit
TCAACGCAGAGTACTTTTTTTTTTT21800.12716182392053904No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT20870.12173703051475457No Hit
AACGCAGAGTACTTTTTTTTTTTTT20820.12144537495530378No Hit
CCTCTAGATAGTCAAGTTCGACCGT19980.11654556155653072No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT19730.11508728375927685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG3400.014.7800975
ATAACGC1101.2732926E-1114.6533383
ACCGCTC400.005348871514.2219428
CCTCTAG4100.014.1075521
GGTATCA63250.013.9870721
TATCACG955.0258677E-913.975292
CGATCAA707.448767E-613.53838710
CGGGGAT655.5792643E-513.121819510
GATAGTC3950.012.961777
GTGGTAT15000.012.8324761
ATAAACG1700.012.8280093
TAGTCAA3700.012.80658159
TATAACG1207.6215656E-1012.644312
ATTATCC604.1599822E-412.6420963
ATCACGC1052.0487278E-812.6420953
CGACCGT3650.012.55132819
TCGACCG3500.012.54385618
GTCCTAA701.1103741E-412.1913041
AGTCAAG4200.012.18454710
TCTAGAT4450.012.1449353
Produced by FastQC (version 0.11.5)