FastQCFastQC Report
Thu 26 May 2016
SRR1364256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3651547
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN82650.2263424241835036No Hit
GTATCAACGCAGAGTACTTTTTTTT53260.14585598925606053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTG550.00307509612.0881312
GGTATCA24700.012.0802861
GTGCACG2100.011.75952311
CGGTTTG1900.011.00109814
GTATCAA61950.010.9368631
GGACCGT1408.38736E-910.8552446
GTTCGGT1106.0658986E-610.36153911
ACGGCCC1752.3646862E-1010.31489115
ACATGGT14500.010.2906273
TTACGCC750.002639606810.1376884
TGGACCG1502.4554538E-810.1314215
GTCCGCG856.6097645E-410.05678758
GTTCTAG4450.09.8229591
GTATTGG5550.09.7594741
GTCTTAG3450.09.6403661
CTCAACG2500.09.5040834
CGTCTCG1502.6485213E-79.50068717
GGACCGA2201.6370905E-119.4983396
AGGACCG2500.09.4982075
GGACAGT5900.09.3373496
Produced by FastQC (version 0.11.5)