FastQCFastQC Report
Thu 26 May 2016
SRR1364256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3651547
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT177130.48508207617209914No Hit
TATCAACGCAGAGTACTTTTTTTTT127230.34842766641097594No Hit
GGTATCAACGCAGAGTACTTTTTTT114130.3125524606420238No Hit
ACGCAGAGTACTTTTTTTTTTTTTT60630.16603921570775346No Hit
GTGGTATCAACGCAGAGTACATGGG50290.1377224502382141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAT551.0710164E-515.63237418
GGTATCA44800.012.5657661
CGTCCGA1155.5624696E-912.35633311
TATCACG854.0492523E-612.2661382
TGCCGTA1103.5337507E-812.15868115
TAACGCA1103.9495717E-812.0619054
TGGTATC14700.011.7351372
GTGGTAT15800.011.5765931
ATAACGC1408.683855E-910.8310993
TCGTCCG1408.742063E-910.82650110
GGACCGA1855.456968E-1210.7577686
AATACCG750.002702332310.1090255
ACCGAAC750.002702639410.1088878
TAGGACA5450.010.085844
CCGACAT1553.7374775E-89.86046514
ACCGAGT2500.09.8561648
AGGACCG2700.09.8282185
GTATCAA92450.09.8206261
TATAACG1451.6737795E-79.8052212
CTTACAC6550.09.6942463
Produced by FastQC (version 0.11.5)