##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364256_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3651547 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.427658195279975 31.0 30.0 33.0 26.0 34.0 2 29.471688574732845 31.0 30.0 34.0 25.0 34.0 3 29.554133905437887 31.0 29.0 34.0 26.0 34.0 4 33.07360715882885 35.0 33.0 37.0 27.0 37.0 5 32.39577170990815 35.0 32.0 37.0 25.0 37.0 6 32.436638224840046 35.0 32.0 37.0 25.0 37.0 7 32.11695673094171 35.0 32.0 37.0 22.0 37.0 8 31.947688472858214 35.0 32.0 37.0 19.0 37.0 9 32.772270766335474 37.0 32.0 39.0 17.0 39.0 10 32.49103160934256 37.0 32.0 39.0 17.0 39.0 11 32.97569167259794 37.0 32.0 39.0 18.0 39.0 12 32.85147199255548 37.0 32.0 39.0 17.0 39.0 13 32.9596023274519 37.0 32.0 39.0 18.0 39.0 14 33.46730741792451 38.0 32.0 40.0 17.0 41.0 15 33.673673377338424 38.0 32.0 40.0 17.0 41.0 16 33.73529137102713 38.0 32.0 40.0 17.0 41.0 17 33.65359558565178 38.0 32.0 40.0 17.0 41.0 18 33.59009674529727 38.0 32.0 40.0 17.0 41.0 19 33.463185329396005 38.0 32.0 40.0 16.0 41.0 20 33.11715281221904 38.0 32.0 40.0 10.0 41.0 21 32.716731292244084 38.0 31.0 40.0 10.0 41.0 22 32.83442387568885 38.0 32.0 40.0 8.0 41.0 23 32.75724535381853 38.0 32.0 40.0 2.0 41.0 24 32.64165790553976 38.0 32.0 40.0 2.0 41.0 25 32.59388308571682 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7184655361394228 1101 2 0.7212817591181917 1101 3 0.7228483864687085 1101 4 0.7178506897494898 1101 5 0.9661412909682738 1101 6 0.7249842449641761 1101 7 1.003586696439445 1101 8 1.033894347362196 1101 9 2.058899661991987 1101 10 2.052580920991563 1101 11 1.1306385530233882 1101 12 1.1644505124377744 1101 13 0.9610740921881202 1101 14 1.4848257719830045 1101 15 1.2538583108134418 1101 16 1.1791247794366626 1101 17 1.1115862453881107 1101 18 1.211827685984879 1101 19 1.1915707221931982 1101 20 2.007870231893982 1101 21 2.2902131945052915 1101 22 2.18671778286145 1101 23 2.0786623045492902 1101 24 1.8658978423640065 1101 25 2.0158290163459895 1102 1 0.437589904687794 1102 2 0.5948350159855984 1102 3 0.5489883715866064 1102 4 0.5013605936087515 1102 5 0.8862352935118736 1102 6 0.59931699625524 1102 7 0.8368598985810429 1102 8 0.8949030431625964 1102 9 2.050895364118766 1102 10 2.056259386119425 1102 11 1.20817273441898 1102 12 1.2645027072509762 1102 13 1.0430691421113352 1102 14 1.6171818869842767 1102 15 1.1154807079283273 1102 16 0.9854383507551248 1102 17 1.05897435542078 1102 18 0.9394334430153322 1102 19 0.9432007678770944 1102 20 1.569175328271882 1102 21 2.2376186842805197 1102 22 2.131792978967205 1102 23 1.9952526028731228 1102 24 1.8987446664291312 1102 25 2.0196665857908442 1103 1 0.5088676698829815 1103 2 0.6408727891233106 1103 3 0.647727803624349 1103 4 0.6632320543812966 1103 5 0.9309782251736323 1103 6 0.6731041642681461 1103 7 1.0410606606618238 1103 8 0.9333678667447209 1103 9 1.8913126057904108 1103 10 1.945538540696628 1103 11 1.187593973821059 1103 12 1.1991296103203908 1103 13 0.9737403120312464 1103 14 1.7076747704238286 1103 15 1.256261003954279 1103 16 1.0087709383411934 1103 17 1.0744105024014061 1103 18 0.7895810438563089 1103 19 0.9055600802550643 1103 20 1.5741309140016 1103 21 2.2295300454556752 1103 22 1.9323341017893156 1103 23 1.7910512061667774 1103 24 1.7508871007460343 1103 25 1.5388623852863432 1104 1 0.28934926978347164 1104 2 0.5064100714413193 1104 3 0.29495700284649473 1104 4 0.3484612408709964 1104 5 0.47031585391214037 1104 6 0.32951223330934454 1104 7 0.5539003537706755 1104 8 0.6435159206147212 1104 9 1.3981109977803357 1104 10 1.4697666728417964 1104 11 0.8487906671067051 1104 12 0.5683335924420803 1104 13 0.6942569849253886 1104 14 1.2797830236447751 1104 15 0.3064337000057975 1104 16 0.3792168237286049 1104 17 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2204 1 0.8782849095409588 2204 2 0.9198818585782647 2204 3 0.909827286724461 2204 4 0.9481162126253011 2204 5 1.2183015282515939 2204 6 1.0970210451915108 2204 7 1.3652192625093456 2204 8 1.2652123709484435 2204 9 2.434812030562604 2204 10 2.4840817024635733 2204 11 1.5583758363425133 2204 12 1.8092290361661014 2204 13 1.349995838422842 2204 14 2.2115236975300334 2204 15 1.793880866937343 2204 16 1.864536295492357 2204 17 1.7553135116569365 2204 18 1.8725656442116474 2204 19 1.8636989901670518 2204 20 2.5648754180264035 2204 21 2.842902168645672 2204 22 2.689857169050711 2204 23 2.727152109891719 2204 24 2.363767265495575 2204 25 2.646776195942465 2205 1 0.40872899107106164 2205 2 0.5299412858017263 2205 3 0.5624295919284421 2205 4 0.4611318563271709 2205 5 0.7849380800070165 2205 6 0.36843784184432593 2205 7 0.39594202037918436 2205 8 0.9088694657493441 2205 9 1.5496246236902635 2205 10 1.655299908201112 2205 11 1.0267268443021962 2205 12 1.215348644358194 2205 13 0.9870296766956486 2205 14 1.0869648652767196 2205 15 0.17182726030159046 2205 16 0.18997062369953 2205 17 0.1334218555461888 2205 18 0.709804005653389 2205 19 0.7990937467527317 2205 20 1.3347935878214656 2205 21 1.4965773742680568 2205 22 0.9056861317089187 2205 23 0.13346605173469328 2205 24 0.18500721119800545 2205 25 0.4120412804421818 2206 1 -1.1226540061330432 2206 2 -3.076752770621596 2206 3 -0.9115288486835524 2206 4 -1.8921321134241929 2206 5 -0.31133606871954456 2206 6 -0.7711648843945689 2206 7 -0.6427650603506727 2206 8 0.13167337744062024 2206 9 1.434141632059145 2206 10 0.9889042023374977 2206 11 0.7218241410781019 2206 12 0.4250697603822218 2206 13 0.33083206673665444 2206 14 0.8789129437123222 2206 15 0.10594754521247296 2206 16 0.5664388661969681 2206 17 0.6327938843077661 2206 18 0.09261097432505494 2206 19 0.5110427159331863 2206 20 1.0676324670778854 2206 21 1.5989934264231067 2206 22 1.3485186747069875 2206 23 1.044602243436664 2206 24 0.9114276169657103 2206 25 0.6269437955683301 2207 1 0.18307919900395575 2207 2 0.27722697258421647 2207 3 0.08657777874282857 2207 4 0.20713474444943358 2207 5 0.44230480490302426 2207 6 0.21895114560838635 2207 7 0.2621305325935914 2207 8 0.5729297528206452 2207 9 0.9282650796880745 2207 10 1.4025565322963764 2207 11 0.2668622594123562 2207 12 0.38459059590771716 2207 13 0.4741325067208919 2207 14 1.3487466660235015 2207 15 0.7524389320468998 2207 16 -0.13062009172175948 2207 17 0.07315817616462539 2207 18 0.47149174253201664 2207 19 0.7384623180508711 2207 20 1.2551477896764283 2207 21 1.6019113981119375 2207 22 1.3537745724432924 2207 23 0.6663703300174362 2207 24 1.081708355190436 2207 25 0.8151827159234415 2208 1 0.2695906574579823 2208 2 0.4627106751079779 2208 3 0.44457834579810296 2208 4 0.3607411694199598 2208 5 0.3740349769149631 2208 6 0.44593668231180317 2208 7 0.8386024100814247 2208 8 0.7971695946209785 2208 9 1.7183639177892829 2208 10 1.8238936311326377 2208 11 1.1163687463242908 2208 12 1.06753052347198 2208 13 0.9107115446716847 2208 14 1.577860353549518 2208 15 1.1997693337599813 2208 16 0.9984829970359428 2208 17 0.9053298906064242 2208 18 0.6565256677639866 2208 19 1.0697721240002664 2208 20 1.7754249552298944 2208 21 2.2430588368581823 2208 22 1.809446670543572 2208 23 1.9435789630317686 2208 24 1.7251427498730436 2208 25 1.4767625265540758 2209 1 0.36872594826074234 2209 2 0.24723166428403687 2209 3 0.18741688378367982 2209 4 0.20877342827484568 2209 5 0.523438557504619 2209 6 0.2659714365349899 2209 7 0.44374241645178003 2209 8 0.3655189386444384 2209 9 1.3472344243829681 2209 10 1.2997418961543268 2209 11 0.2929509210264598 2209 12 0.7283960242513174 2209 13 0.4496206314686617 2209 14 0.8154772768207081 2209 15 0.6002891393323395 2209 16 0.3950090824928836 2209 17 0.16960616783354965 2209 18 0.21281280851293616 2209 19 0.15683107613405411 2209 20 1.0523231973522371 2209 21 1.5916357795796756 2209 22 1.50411366892083 2209 23 1.30533857462882 2209 24 0.822324529161115 2209 25 0.7782035829015328 2210 1 -0.10826895202495734 2210 2 0.22447740550734352 2210 3 0.28346877312210594 2210 4 0.2987763816068565 2210 5 0.12620626829201598 2210 6 0.32376510666211544 2210 7 0.4594969863716436 2210 8 0.4993991689601778 2210 9 1.574897634433384 2210 10 1.5411437261376868 2210 11 0.7393692029990859 2210 12 0.7634910375783406 2210 13 0.6043680361222172 2210 14 1.1974018415852399 2210 15 0.6345069126367946 2210 16 0.7891971323302016 2210 17 0.6004064723821187 2210 18 0.35123817549131786 2210 19 0.6598966269297577 2210 20 1.3457920855626782 2210 21 1.7777675712551826 2210 22 1.587990719437336 2210 23 1.722303334120987 2210 24 1.5738539128918063 2210 25 1.4462984352089379 2211 1 0.2899606779915729 2211 2 0.19960533771351052 2211 3 0.09328648472726897 2211 4 0.17273298417919847 2211 5 0.3031003354844799 2211 6 -0.08720148909187486 2211 7 0.3047428491323281 2211 8 0.37118279448479186 2211 9 1.3685773952407487 2211 10 1.3315604216258627 2211 11 0.600127366309053 2211 12 -0.0250125685217526 2211 13 -0.26445172663324 2211 14 0.49453630133371007 2211 15 0.2608193735512998 2211 16 0.26909169501733743 2211 17 0.06508137282439463 2211 18 -0.12414315071215754 2211 19 -0.09326846878823147 2211 20 0.5114951021511587 2211 21 1.3842234009405345 2211 22 1.2411506625754427 2211 23 1.2125644640464657 2211 24 1.1229572934077154 2211 25 0.8199926797066155 2212 1 0.9253053918333585 2212 2 1.0502254722959101 2212 3 0.8784439752523596 2212 4 1.054058880143998 2212 5 1.0748539559693953 2212 6 1.1743811052818742 2212 7 1.7282697451380749 2212 8 1.4292088283078321 2212 9 -9.558723995626032 2212 10 -0.9559604504753594 2212 11 0.48929412017977114 2212 12 1.371772786656578 2212 13 1.4545474589567036 2212 14 2.19394278294579 2212 15 1.824246902431014 2212 16 1.665491911586713 2212 17 1.8267658821992043 2212 18 1.997054259554865 2212 19 2.0320241956065743 2212 20 2.6590163837323004 2212 21 2.849284527192296 2212 22 2.7653450108426583 2212 23 2.6884754431129423 2212 24 2.4942099834632643 2212 25 2.5490452796765837 2213 1 0.47372546551018146 2213 2 0.42052387065735886 2213 3 0.19539814798035593 2213 4 0.4169145083148891 2213 5 0.48453260295995904 2213 6 0.24656973386529302 2213 7 0.5494146790726013 2213 8 0.83271547345527 2213 9 1.4498728396963827 2213 10 -8.915110404759062 2213 11 -2.6901978378529066 2213 12 -0.8749010209200243 2213 13 0.0673533299426552 2213 14 0.5142683819016725 2213 15 0.40082924122376085 2213 16 0.7531538184509117 2213 17 0.6242731377014508 2213 18 0.4497047192509456 2213 19 0.8761479022213265 2213 20 1.6567935945797245 2213 21 1.5600176646331008 2213 22 1.8252894516683966 2213 23 1.4186524821925346 2213 24 1.4634036516956286 2213 25 1.0771249177096536 2214 1 -2.1997582237962945 2214 2 -2.300744312636141 2214 3 -2.332599698055464 2214 4 -2.360630204356408 2214 5 -2.4312342954018753 2214 6 -2.4328041622807532 2214 7 -10.338748142634525 2214 8 -4.098678378824658 2214 9 -3.393436452979998 2214 10 -2.961577474960311 2214 11 -3.5593459190263026 2214 12 -12.376931339406038 2214 13 -5.873275661319244 2214 14 -13.663727848309748 2214 15 -7.526817127878303 2214 16 -5.408255096122559 2214 17 -4.657985215759439 2214 18 -4.394290244997183 2214 19 -4.786185896188421 2214 20 -4.866156556534982 2214 21 -13.649820141723694 2214 22 -16.218086651834284 2214 23 -10.749454551420829 2214 24 -9.126706835566761 2214 25 -16.431021823032026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 118440.0 3 4.0 4 1924.0 5 17991.0 6 22252.0 7 18021.0 8 14752.0 9 12454.0 10 10689.0 11 9851.0 12 9206.0 13 9194.0 14 9061.0 15 9213.0 16 9583.0 17 10568.0 18 11927.0 19 14264.0 20 18186.0 21 23903.0 22 30091.0 23 36534.0 24 43094.0 25 50235.0 26 57727.0 27 65559.0 28 73539.0 29 86904.0 30 103453.0 31 126590.0 32 155339.0 33 190960.0 34 231339.0 35 277288.0 36 344699.0 37 464487.0 38 962226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.93668211568769 19.7641912712803 13.972849206177454 24.326277406854565 2 16.125538754144053 20.713357596899055 38.56496383472437 24.596139814232533 3 19.261382588734815 25.0856771659193 28.767262093529833 26.88567815181605 4 12.929506316090139 17.23850192808692 36.33309389143834 33.4988978643846 5 13.832617674308548 37.9722041850648 33.86831344468566 14.326864695940996 6 32.099165740792216 37.10855502514992 17.25563418679924 13.536645047258622 7 28.994531870674233 31.8047565358345 20.892953433402994 18.30775816008827 8 26.467482581666847 35.337640312447775 19.229757609133426 18.965119496751957 9 26.16790666058309 15.934629324619143 19.719710069831475 38.17775394496629 10 14.9038881553849 30.341540818469948 31.830385030712684 22.924185995432467 11 34.826314421576186 23.44247968654189 22.385706073593205 19.345499818288726 12 23.243626070081323 26.314346607481383 29.475745736243887 20.96628158619341 13 28.62218672985185 21.7751443315316 25.952344372391643 23.6503245662249 14 22.26698306588653 21.384141607364633 27.539083199940844 28.809792126807988 15 24.339833511386015 29.449785720603987 23.184923844481002 23.025456923529 16 23.462725741114404 28.724577765801058 25.281224736603413 22.53147175648112 17 22.460181116048847 28.54279823117861 26.357897954002645 22.639122698769892 18 21.972989293485032 27.644224383577775 28.335252628018782 22.04753369491841 19 23.377515037919327 26.882026687582638 27.867721082883556 21.872737191614476 20 23.306578825276052 26.439546079172132 28.243652708369233 22.01022238718258 21 24.022044302254557 25.701032367982073 27.55948647293903 22.71743685682434 22 23.55871053758093 26.154084640587943 27.712629206565353 22.574575615265772 23 23.102592157931422 26.117487240513746 27.860637789442116 22.919282812112712 24 22.97417982514877 26.465891467136178 27.79681589243935 22.763112815275697 25 23.020914635315254 26.28285284717829 28.06523876423726 22.630993753269195 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 505.0 1 505.0 2 814.5 3 1124.0 4 1124.0 5 1124.0 6 2992.5 7 4861.0 8 4861.0 9 4861.0 10 6257.0 11 7653.0 12 7653.0 13 7653.0 14 10468.5 15 13284.0 16 13284.0 17 13284.0 18 25904.0 19 38524.0 20 38524.0 21 38524.0 22 61067.5 23 83611.0 24 83611.0 25 83611.0 26 122194.5 27 160778.0 28 160778.0 29 160778.0 30 198653.0 31 236528.0 32 236528.0 33 236528.0 34 274486.0 35 312444.0 36 312444.0 37 312444.0 38 346224.0 39 380004.0 40 380004.0 41 380004.0 42 406091.0 43 432178.0 44 432178.0 45 432178.0 46 455632.0 47 479086.0 48 479086.0 49 479086.0 50 469581.5 51 460077.0 52 460077.0 53 460077.0 54 420883.0 55 381689.0 56 381689.0 57 381689.0 58 333937.5 59 286186.0 60 286186.0 61 286186.0 62 236068.5 63 185951.0 64 185951.0 65 185951.0 66 144437.0 67 102923.0 68 102923.0 69 102923.0 70 76250.0 71 49577.0 72 49577.0 73 49577.0 74 34717.5 75 19858.0 76 19858.0 77 19858.0 78 14647.5 79 9437.0 80 9437.0 81 9437.0 82 6435.0 83 3433.0 84 3433.0 85 3433.0 86 2365.0 87 1297.0 88 1297.0 89 1297.0 90 898.0 91 499.0 92 499.0 93 499.0 94 267.5 95 36.0 96 36.0 97 36.0 98 20.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02864539330864425 2 0.014541781880392065 3 0.0013418970096783637 4 0.0 5 0.0036696775366714435 6 0.0012049687433846532 7 6.572556782098108E-4 8 0.04537802744973569 9 0.005066345852867291 10 0.0033958210040840224 11 0.004107847988811318 12 0.00517588846590226 13 0.001588367889007043 14 0.001150197436867169 15 1.643139195524527E-4 16 5.203274119161002E-4 17 0.0020813096476644008 18 8.763409042797477E-4 19 0.003039807511720375 20 0.8345777830601661 21 0.0196355133865181 22 0.008763409042797477 23 0.009365893414489804 24 0.002738565325874212 25 0.0010132691705734584 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3651547.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.18052375227748 #Duplication Level Percentage of deduplicated Percentage of total 1 70.02472460845026 30.23704284201876 2 14.46558223737714 12.492628347831735 3 5.846391485282018 7.573507391859989 4 2.9668522964774824 5.124409442301796 5 1.6808966416738922 3.629099868046147 6 1.1218249057655798 2.906459219358424 7 0.7403530489902117 2.2378182691895026 8 0.5169370827974435 1.7857291185734432 9 0.38049633128734345 1.4787027783726816 >10 1.9797425346983495 15.635863458643232 >50 0.1693473691483257 5.078808163199082 >100 0.09999118322737584 7.896375416270707 >500 0.005226876059094556 1.5149497621589727 >1k 0.0013067190123846738 0.9150129532869345 >5k 1.3067190123846738E-4 0.3129320037572435 >10k+ 1.9600785185770105E-4 1.1806609651313453 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 17713 0.48508207617209914 No Hit TATCAACGCAGAGTACTTTTTTTTT 12723 0.34842766641097594 No Hit GGTATCAACGCAGAGTACTTTTTTT 11413 0.3125524606420238 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 6063 0.16603921570775346 No Hit GTGGTATCAACGCAGAGTACATGGG 5029 0.1377224502382141 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.7385653258742117E-5 0.0 0.0 0.0 0.0 3 2.7385653258742117E-5 0.0 0.0 0.0 0.0 4 2.7385653258742117E-5 0.0 0.0 0.0 0.0 5 5.4771306517484234E-5 0.0 0.0 0.0 0.0 6 5.4771306517484234E-5 0.0 0.0 0.0 2.7385653258742117E-5 7 5.4771306517484234E-5 0.0 0.0 0.0 2.7385653258742117E-5 8 5.4771306517484234E-5 0.0 0.0 0.0 2.7385653258742117E-5 9 5.4771306517484234E-5 0.0 0.0 0.0 2.7385653258742117E-5 10 5.4771306517484234E-5 0.0 0.0 0.0 2.7385653258742117E-5 11 8.215695977622635E-5 0.0 0.0 0.0 2.7385653258742117E-5 12 8.215695977622635E-5 0.0 0.0 0.0 2.7385653258742117E-5 13 8.215695977622635E-5 0.0 0.0 2.7385653258742117E-5 2.7385653258742117E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTAT 55 1.0710164E-5 15.632374 18 GGTATCA 4480 0.0 12.565766 1 CGTCCGA 115 5.5624696E-9 12.356333 11 TATCACG 85 4.0492523E-6 12.266138 2 TGCCGTA 110 3.5337507E-8 12.158681 15 TAACGCA 110 3.9495717E-8 12.061905 4 TGGTATC 1470 0.0 11.735137 2 GTGGTAT 1580 0.0 11.576593 1 ATAACGC 140 8.683855E-9 10.831099 3 TCGTCCG 140 8.742063E-9 10.826501 10 GGACCGA 185 5.456968E-12 10.757768 6 AATACCG 75 0.0027023323 10.109025 5 ACCGAAC 75 0.0027026394 10.108887 8 TAGGACA 545 0.0 10.08584 4 CCGACAT 155 3.7374775E-8 9.860465 14 ACCGAGT 250 0.0 9.856164 8 AGGACCG 270 0.0 9.828218 5 GTATCAA 9245 0.0 9.820626 1 TATAACG 145 1.6737795E-7 9.805221 2 CTTACAC 655 0.0 9.694246 3 >>END_MODULE