FastQCFastQC Report
Thu 26 May 2016
SRR1364255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430949
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN32180.22488572269172413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA902.2955646E-914.7737456
AGGACCG1153.783498E-1013.213325
GCCCCCG2150.012.815845518
TAGTACC701.0864281E-412.2195164
GTCCTAC951.0346375E-612.0047181
AGACCCG1353.7289283E-1011.9592785
GGTTTAG1406.7484507E-1011.540251
GTATTAC752.0660306E-411.4044821
GTCTTAG1604.5474735E-1111.2856851
GGCGTAG1104.9817936E-711.22460110
ACATGGT6100.011.0621123
TCAACTC5000.011.0247184
GGTATCA8200.011.0104241
GTCTTAA1658.0035534E-1110.9436941
ACTCATA1658.0035534E-1110.9364087
TCCCGAC700.001486389710.8614112
TACTAGA700.001486389710.8614112
CGCGCCA1053.47485E-610.8545610
CGACCCG700.001495109510.8537995
TTAGTAC803.7553778E-410.6920773
Produced by FastQC (version 0.11.5)