FastQCFastQC Report
Thu 26 May 2016
SRR1364255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430949
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAA902.3719622E-914.7399881
CTCGACG456.909024E-414.735867513
GGACCGA1051.4169927E-913.540516
TCGCGGT802.7097361E-511.94020817
CCAATAC802.934704E-511.8475323
GCCCCCG2100.011.82607518
AAGACCG1052.8007344E-711.7351095
CCTATAA2250.011.3708481
TATAAGC2600.011.3007233
CGCGGTT855.0349146E-511.23744718
ACGGCCC2100.010.91676115
CGTGGCG700.001519917410.8324096
AGTACCG700.001519917410.8324095
AGGACCG1605.657057E-1010.6631525
AATATAG803.8505677E-410.6631525
TAGACGT803.8705158E-410.6571913
GGTATCA11850.010.6351811
TCAATAC909.7388795E-510.531143
TTCGCGG1002.2573226E-510.50738316
TAGGGTG1451.4942998E-810.4588775
Produced by FastQC (version 0.11.5)