FastQCFastQC Report
Thu 26 May 2016
SRR1364254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2352372
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT96310.4094165378605084No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA67760.2880496792174027No Hit
TATCAACGCAGAGTACTTTTTTTTT66790.2839261817433637No Hit
GGTATCAACGCAGAGTACTTTTTTT52030.22118100368479135No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN51010.21684495479456478No Hit
GTACTTTTTTTTTTTTTTTTTTTTT48000.20404935954007275No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27310.11609558352165389No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25180.10704089319206316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT802.1827873E-1117.8168819
GTTCGAC855.638867E-1116.76954316
TCGACCG855.638867E-1116.76882718
TATAACG2150.014.5834242
TAACGCA2300.013.2203594
TTCGACC1102.739398E-912.9577317
ATAACGC2500.012.5428153
CACCGGA1001.4494435E-712.346189513
AGTGTCG550.003074874412.08795310
GGTATCA36950.012.0598651
ATCAGCG1353.7107384E-1011.9656494
CGTAAGA658.016729E-411.6939352
CAACCGT1302.6266207E-911.6899466
ACAACCG1302.6266207E-911.6896975
CATGGTA9450.011.6639934
CGGAATG907.4410455E-611.61446116
ACATGGT11750.011.4834283
AAGTTCG1104.9545633E-711.23027214
CGGTTTG2500.011.02294414
GTATCAA92400.011.0128971
Produced by FastQC (version 0.11.5)