FastQCFastQC Report
Thu 26 May 2016
SRR1364254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2352372
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT324601.379883793889742No Hit
TATCAACGCAGAGTACTTTTTTTTT246981.0499189754001492No Hit
GGTATCAACGCAGAGTACTTTTTTT206300.8769871431899376No Hit
GTACTTTTTTTTTTTTTTTTTTTTT150280.6388445364933777No Hit
ACGCAGAGTACTTTTTTTTTTTTTT110900.47143904110404306No Hit
GAGTACTTTTTTTTTTTTTTTTTTT95260.40495295812056936No Hit
ATCAACGCAGAGTACTTTTTTTTTT42680.18143388885771466No Hit
GCAGAGTACTTTTTTTTTTTTTTTT42350.18003104951087667No Hit
GTGGTATCAACGCAGAGTACATGGG39970.1699136021003481No Hit
GTGGTATCAACGCAGAGTACTTTTT36630.155715167499018No Hit
GGTATCAACGCAGAGTACATGGGAT33410.14202685629653813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2400.016.983972
CGGATCG350.002097791516.3691516
TAACGCA2700.014.3940714
CGACCGT602.4494615E-514.322391519
ATAACGC3000.014.2185333
TCGACCG706.894197E-613.64066518
GGTATCA59450.012.9612471
TATCACG1501.8189894E-1212.6392342
TCGTTGC751.5186513E-512.634397511
CGTAGTC802.721675E-511.93583815
CAATCGT1156.594746E-811.62446716
TAATACC2450.011.6069664
AAGTTCG751.979672E-411.45766714
ATACGCT752.1164546E-411.3755528
ACGGCCC1850.011.35517515
TTCGTTG700.00152397710.82948410
CGTCTTA1158.259922E-710.794147515
GTTCGAC803.598199E-410.74225316
CTTACTC1159.076648E-710.7154163
ACGCCGA2250.010.5329196
Produced by FastQC (version 0.11.5)