FastQCFastQC Report
Thu 26 May 2016
SRR1364253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283484
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN25570.19922336390636736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACG655.42374E-513.1608824
AGGACCG604.1010245E-412.6630485
CGTCTCG853.933723E-612.29636817
GTCTTAC1103.7827704E-812.0973761
ACATGGT7400.012.0740213
CTTATTC1052.6994167E-711.7681963
CGGTTTG658.012043E-411.69399214
ACCGAGT658.043612E-411.6885128
CCTAGAC1157.015842E-811.57140353
TTGCGCG3050.011.526718
TGCGTGA600.005888920311.07973410
GTATAAA1900.011.0058831
CATGGTA5450.010.9875244
TGCGCGC3200.010.98638619
GGTATCA11050.010.9243931
ATTTGCG3050.010.90320916
CATGTTA1502.1118467E-910.7724264
GGACCGA803.779662E-410.6844476
TACATGT8200.010.663822
TAGACAG2050.010.6554925
Produced by FastQC (version 0.11.5)