FastQCFastQC Report
Thu 26 May 2016
SRR1364253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283484
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG15260.11889513231173898No Hit
GTATCAACGCAGAGTACTTTTTTTT15130.1178822642120977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCCG402.8074402E-416.5884045
CCCGTCT400.00535616914.2186328
CGTGCGC400.005374199714.21142910
AGGCGTG752.1161429E-411.3749057
TTGACCG951.2818531E-511.0585518
ATACGAC600.005994415411.05333413
CCACGTC600.005994415411.0533349
GGTGGTA2600.010.9339981
AAAGGCG803.8472476E-410.6639745
ACCGAGT909.726044E-510.5323198
TAGACTA1002.4564886E-510.4269955
GGATATG3050.010.2528641
AGGACCG1303.955447E-710.2082485
TTTTATA1409.982796E-810.1569572
TAGATTG750.002695861310.1110275
TGACCGA951.5581866E-410.05283219
AAGGCGT951.6802612E-49.9779866
TCTATAC1151.0533917E-59.8908373
TTAGACT1252.6410307E-69.8582514
TGTACTG1752.6684575E-99.7499185
Produced by FastQC (version 0.11.5)