##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364253_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1283484 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.205863882993476 31.0 29.0 33.0 25.0 34.0 2 29.241567483505833 31.0 28.0 34.0 25.0 34.0 3 29.316913962308842 31.0 28.0 34.0 25.0 34.0 4 32.860102658077544 35.0 32.0 37.0 27.0 37.0 5 32.11231304792268 35.0 32.0 37.0 22.0 37.0 6 32.13583028693774 35.0 32.0 37.0 23.0 37.0 7 31.79329855300105 35.0 32.0 37.0 19.0 37.0 8 31.624831318504945 35.0 32.0 37.0 18.0 37.0 9 32.34792408787332 37.0 32.0 39.0 15.0 39.0 10 32.02353515898913 37.0 31.0 39.0 11.0 39.0 11 32.59326255722705 37.0 32.0 39.0 17.0 39.0 12 32.450670986159544 37.0 32.0 39.0 17.0 39.0 13 32.583537465211876 37.0 32.0 39.0 17.0 39.0 14 33.02300457193078 38.0 32.0 40.0 11.0 41.0 15 33.24202093676275 38.0 32.0 40.0 16.0 41.0 16 33.30447672117455 38.0 32.0 40.0 16.0 41.0 17 33.20607268964786 38.0 32.0 40.0 11.0 41.0 18 33.14061336175597 38.0 32.0 40.0 10.0 41.0 19 32.98950902387564 38.0 32.0 40.0 10.0 41.0 20 32.65581495367297 38.0 32.0 40.0 2.0 41.0 21 32.18794702544013 38.0 31.0 40.0 2.0 41.0 22 32.232983815926026 38.0 31.0 40.0 2.0 41.0 23 32.09541217498621 38.0 31.0 40.0 2.0 41.0 24 32.05450554895893 38.0 31.0 40.0 2.0 41.0 25 31.983713080957767 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7829798321846226 1101 2 0.685397267264328 1101 3 0.7700744840638158 1101 4 0.8140413925424141 1101 5 0.9940833178560666 1101 6 0.8242644467990345 1101 7 1.2337282287484221 1101 8 1.188091240024022 1101 9 2.079578564147816 1101 10 2.2625499798373276 1101 11 1.514186216293389 1101 12 1.5656348047074928 1101 13 1.3668423789560933 1101 14 1.7591644729160123 1101 15 1.491567239619613 1101 16 1.3675091815020224 1101 17 1.3387687045638117 1101 18 1.434717821696161 1101 19 1.3674896779055246 1101 20 2.1547846494138057 1101 21 2.521503900845971 1101 22 2.4717672772791843 1101 23 2.2653252965793413 1101 24 2.081726905727052 1101 25 2.1021276815791694 1102 1 0.03240100696840287 1102 2 0.3181193889173315 1102 3 0.2734148040934343 1102 4 0.24229067223349432 1102 5 0.6108657702734348 1102 6 0.2871964923769781 1102 7 0.8223995305724578 1102 8 0.7813167330582402 1102 9 1.8242792150660492 1102 10 1.890703777440148 1102 11 1.083523171775255 1102 12 1.0882930086502753 1102 13 0.9395128210064811 1102 14 1.307892952172729 1102 15 0.8573266918493729 1102 16 0.7341635065237995 1102 17 0.8291016866986638 1102 18 0.8253922283964314 1102 19 0.8045233718385276 1102 20 1.4462949747735223 1102 21 1.884947586293059 1102 22 1.8150907683577913 1102 23 1.5663550991668629 1102 24 1.622708569687255 1102 25 1.6696821908559016 1103 1 0.32816233877388257 1103 2 0.3834309223216117 1103 3 0.4995619190328391 1103 4 0.6263611513058507 1103 5 0.7675964569017211 1103 6 0.5579017384523866 1103 7 0.7104740542955845 1103 8 0.6956013698791779 1103 9 1.4168710948131107 1103 10 1.5463842600442241 1103 11 0.7591360150749225 1103 12 0.8514837761809702 1103 13 0.6436011135670512 1103 14 1.2807893480552863 1103 15 1.051943118971252 1103 16 0.75278645691251 1103 17 0.8300740427750171 1103 18 0.47239176506422353 1103 19 0.5897195244129989 1103 20 1.1394556061508894 1103 21 1.839490017962511 1103 22 1.5325183654631331 1103 23 1.4647718988009544 1103 24 1.590070641509854 1103 25 1.3490622489187345 1104 1 0.501733372583562 1104 2 0.7612892589763334 1104 3 0.5207485760222923 1104 4 0.5537663680585894 1104 5 0.859962620778262 1104 6 0.760479675360898 1104 7 1.0596003942263472 1104 8 1.0093221785161148 1104 9 1.78031698122129 1104 10 1.9094674095517554 1104 11 1.2749742052460888 1104 12 0.9294449411371559 1104 13 1.1066258890681056 1104 14 1.559956137589566 1104 15 0.7323363967969598 1104 16 0.9036810441106269 1104 17 0.388826798576666 1104 18 0.9226574920393844 1104 19 1.3158992992307503 1104 20 1.7024912891251844 1104 21 2.2238841189219585 1104 22 2.299387613078949 1104 23 2.0553705129811135 1104 24 1.899554340703304 1104 25 2.032610925579057 1105 1 -0.5235659091402987 1105 2 0.031192348703843464 1105 3 -0.06562241930321022 1105 4 0.26629401598926705 1105 5 0.34783629538777205 1105 6 -0.061458530088877694 1105 7 -0.05126951798434476 1105 8 0.3181375929500483 1105 9 1.1861954443383809 1105 10 1.0542650381003789 1105 11 0.38411797448562623 1105 12 0.7478784150853954 1105 13 0.2676748002526921 1105 14 0.4405170770491509 1105 15 0.5549632352418357 1105 16 -0.024931428811505896 1105 17 0.2232356459144853 1105 18 -0.1261419607026042 1105 19 0.1946930513684748 1105 20 0.8272410325979891 1105 21 1.4073022012386787 1105 22 0.5581540023162077 1105 23 0.7375618252602152 1105 24 0.6919976196908735 1105 25 0.4293661416319594 1106 1 0.11027517772672368 1106 2 0.13242389607783878 1106 3 -0.35233182352599357 1106 4 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16 1.472208931919944 1107 17 1.3177997100722578 1107 18 1.2292696351283894 1107 19 1.6202563041925373 1107 20 1.9950722751867715 1107 21 2.5712303045901166 1107 22 2.211756948081664 1107 23 2.1041598406131428 1107 24 1.839488102122381 1107 25 2.098172232875797 1108 1 -0.5085031476340234 1108 2 -0.2235698406907396 1108 3 0.03322140274443797 1108 4 -1.416284756105341 1108 5 -0.18228410423651553 1108 6 -0.14088641839901328 1108 7 0.03206580777775869 1108 8 0.03688748621279103 1108 9 1.2822196950428477 1108 10 0.7701031390311321 1108 11 0.1946892322774474 1108 12 0.40032654077919005 1108 13 -0.003938213153510617 1108 14 0.5729225078407083 1108 15 0.5083267396394078 1108 16 0.4272639430610923 1108 17 -0.07050528141878942 1108 18 0.018586926668241688 1108 19 0.01814963065290698 1108 20 0.7617402213948523 1108 21 1.0334434359752507 1108 22 1.124771712161646 1108 23 0.8543153048947474 1108 24 0.8478835389121215 1108 25 0.5633478682138033 1109 1 0.015129207806765521 1109 2 0.2275029681506382 1109 3 -0.21571763506698716 1109 4 0.25239904642984357 1109 5 0.1917233093644768 1109 6 0.15676863110797257 1109 7 0.35395704863737976 1109 8 0.5908058953961799 1109 9 1.566557046508997 1109 10 1.7598326061206144 1109 11 0.536740385780881 1109 12 0.7043451314395668 1109 13 0.6055217683382423 1109 14 0.8411041153113104 1109 15 0.46165865366361913 1109 16 0.4155740437919846 1109 17 -0.16124343090294957 1109 18 0.5026552389322774 1109 19 0.6741199144403325 1109 20 1.3034200080864196 1109 21 1.5433110109997656 1109 22 1.3474154730103507 1109 23 0.954950216808875 1109 24 0.79234213829438 1109 25 0.9701253554529004 1110 1 0.7880993486788022 1110 2 0.7651592609125153 1110 3 0.6264026984361912 1110 4 0.8837460145401721 1110 5 1.205155459739899 1110 6 0.8454874909230767 1110 7 0.8110466274087251 1110 8 1.2033181267020083 1110 9 2.0360251750472287 1110 10 1.7532482526879924 1110 11 0.9343172257837793 1110 12 1.3199373451663092 1110 13 1.1059600315049565 1110 14 1.7968267014906374 1110 15 1.0007596366426341 1110 16 1.1182831587986897 1110 17 1.0852571409030105 1110 18 1.2749835072360511 1110 19 1.277907638978668 1110 20 2.181608862570215 1110 21 2.0900868890783144 1110 22 2.24780018031403 1110 23 2.1736806514889295 1110 24 1.855900529967414 1110 25 2.099654754118376 1111 1 -0.024881698613985037 1111 2 0.1378270157285506 1111 3 -0.2354122957801188 1111 4 0.022951507933413495 1111 5 0.0646552400709055 1111 6 -0.3876303211737344 1111 7 -0.03932402926253786 1111 8 -0.21593162026155355 1111 9 1.0600233175929432 1111 10 0.7857436229553763 1111 11 -0.08735423281834898 1111 12 0.031227636352852528 1111 13 -0.13974564818984447 1111 14 -0.014279512639362224 1111 15 0.04433890656299866 1111 16 -0.13705146103492183 1111 17 -0.7828549153100042 1111 18 -0.44054549185190695 1111 19 0.05000488004731096 1111 20 0.2500354069266706 1111 21 1.0743430175652122 1111 22 1.0965441741744186 1111 23 0.8899517285899634 1111 24 0.7428488549218102 1111 25 1.0135622345428104 1112 1 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1 0.7310919957688604 2204 2 0.7801730449637958 2204 3 0.7614460470473787 2204 4 0.7933702133023672 2204 5 1.066824328173361 2204 6 0.9021677646501232 2204 7 1.175289651489397 2204 8 1.281400848000814 2204 9 2.221253939325088 2204 10 2.214618926853408 2204 11 1.3929225941301553 2204 12 1.5178945869967606 2204 13 1.1287961053653817 2204 14 2.014196305215094 2204 15 1.6969306863119442 2204 16 1.5955341644609788 2204 17 1.4919355031283388 2204 18 1.5876669583037284 2204 19 1.7782687004143298 2204 20 2.4475794594260094 2204 21 2.7342289858682634 2204 22 2.5184900625230497 2204 23 2.602530309754897 2204 24 2.3574870763935394 2204 25 2.529014146175875 2205 1 0.6855320690867615 2205 2 0.7514072215892362 2205 3 0.7311059267786106 2205 4 0.7024158301892882 2205 5 0.940478247969736 2205 6 0.6316569680543935 2205 7 0.8148469005893055 2205 8 1.2450097166051108 2205 9 1.7835208006084144 2205 10 1.8584247486408962 2205 11 1.2935634945287902 2205 12 1.436688520776194 2205 13 1.4026600416276196 2205 14 1.5735732233023612 2205 15 0.26449064184988913 2205 16 0.4301934640694469 2205 17 0.4392606562296564 2205 18 0.8554284839604875 2205 19 0.9489603570465803 2205 20 1.604137912656391 2205 21 1.6009759880162235 2205 22 1.1791467975680519 2205 23 0.34377631727786095 2205 24 0.23515977413274314 2205 25 0.46769952786358715 2206 1 -1.307459104661401 2206 2 -3.421986297431612 2206 3 -0.9640555083010831 2206 4 -2.099184723310067 2206 5 -0.6164935399055196 2206 6 -1.0617928653733806 2206 7 -0.8297511225124587 2206 8 0.009122141808351358 2206 9 1.040945220217722 2206 10 0.7565417524036739 2206 11 0.3845627001795222 2206 12 0.288539702856319 2206 13 0.18206000653158583 2206 14 0.6060745661946783 2206 15 -0.07977001723227772 2206 16 0.3610261376776265 2206 17 0.4712027397287528 2206 18 -0.10926382960520442 2206 19 0.3659809980717057 2206 20 0.9941516831631247 2206 21 1.3050708384093141 2206 22 1.2351256488559557 2206 23 0.7453225522900411 2206 24 0.6220336547767431 2206 25 0.5358812851570676 2207 1 0.48916206912900506 2207 2 0.42834645132277416 2207 3 0.3369124060355375 2207 4 0.4625114179675265 2207 5 0.6968949223309835 2207 6 0.35870540044258803 2207 7 0.2817289852586313 2207 8 0.5642625761214326 2207 9 1.2025371801013733 2207 10 1.55749011392815 2207 11 0.32344863107888244 2207 12 0.46321550219032304 2207 13 0.5444934392333138 2207 14 1.4740895747327372 2207 15 0.8554520174834863 2207 16 0.09799017754932038 2207 17 0.21875487805437643 2207 18 0.7246951937167907 2207 19 0.7687017468140525 2207 20 1.3667796513050803 2207 21 1.6154276120549298 2207 22 1.6820417692972711 2207 23 0.9518990153166769 2207 24 1.24918910163872 2207 25 1.0640804511042958 2208 1 0.15728015431445996 2208 2 0.5235203821498899 2208 3 0.47939400794924225 2208 4 0.3156112658034118 2208 5 0.4508245365126555 2208 6 0.3178714298622012 2208 7 0.6836328377875738 2208 8 0.7070091086538639 2208 9 1.547461342001796 2208 10 1.5947085339476068 2208 11 0.8287561352728545 2208 12 0.8499403350355195 2208 13 0.6671654355197205 2208 14 1.360256958900834 2208 15 1.0882868161430537 2208 16 0.9244879817259175 2208 17 0.641189713557992 2208 18 0.4667950401847065 2208 19 0.6001160431268673 2208 20 1.5492826483042066 2208 21 1.9843998134915708 2208 22 1.62699274345308 2208 23 1.6162000020350362 2208 24 1.2008536274288701 2208 25 1.020962422917684 2209 1 0.3149076111089215 2209 2 0.3957304630149885 2209 3 0.28790863797951616 2209 4 0.2642487492844552 2209 5 0.5525387558111916 2209 6 0.3951760302773266 2209 7 0.5525711584731532 2209 8 0.4843509568002595 2209 9 1.4236126355139191 2209 10 1.2592062295302249 2209 11 0.37432237400243906 2209 12 0.8950654581269148 2209 13 0.5727520461042985 2209 14 0.9817879779328678 2209 15 0.6417780359284677 2209 16 0.5912800277337311 2209 17 0.17905610493921387 2209 18 0.2261916269380606 2209 19 0.1369748238905686 2209 20 1.0157180495730032 2209 21 1.6494987808050894 2209 22 1.696760398145564 2209 23 1.4917102135875098 2209 24 0.7608128654153319 2209 25 0.7956205125392017 2210 1 -0.06809595536526203 2210 2 0.23687235876642276 2210 3 0.300360096969861 2210 4 0.2899652841883835 2210 5 0.14632250651320788 2210 6 0.3164405925802214 2210 7 0.49363913268701864 2210 8 0.26898127711092457 2210 9 1.5625905262540414 2210 10 1.593693986875575 2210 11 0.7764861390087532 2210 12 0.9452895027870412 2210 13 0.7259352323145762 2210 14 1.3610258660809365 2210 15 0.604150053266423 2210 16 0.7653160476829441 2210 17 0.7093807356915391 2210 18 0.6028404612338107 2210 19 0.7529176355010492 2210 20 1.4417239104949147 2210 21 1.6488807679504234 2210 22 1.5776867214130874 2210 23 1.547025364142641 2210 24 1.6214221660887844 2210 25 1.411610725135624 2211 1 -0.02191015249801964 2211 2 -0.034360211943056385 2211 3 -0.09376258102827961 2211 4 -0.013474948200176584 2211 5 -0.1875814943696419 2211 6 -0.3521631893110566 2211 7 -0.011192506047493112 2211 8 -0.013914775167009452 2211 9 0.992618239871689 2211 10 1.0715388155358845 2211 11 0.27318227945289664 2211 12 -0.16793213554995035 2211 13 -0.5759601631666413 2211 14 0.1712448398523847 2211 15 -0.027947815971103296 2211 16 0.06306241023354886 2211 17 -0.32670659513287603 2211 18 -0.4558916719078425 2211 19 -0.2965660937990293 2211 20 0.2782383371801984 2211 21 1.0662086317354067 2211 22 0.9603565437101196 2211 23 0.9751436504431155 2211 24 0.804573668978513 2211 25 0.43857676927924416 2212 1 0.8537068181324265 2212 2 0.9140680444366822 2212 3 0.6774016994351939 2212 4 0.6011363165458548 2212 5 0.9226322137551151 2212 6 0.9115099300224401 2212 7 1.114055157340335 2212 8 1.0015698749537592 2212 9 -10.121346299631934 2212 10 -1.3319223459441432 2212 11 -0.4013644002824641 2212 12 1.379149287070554 2212 13 1.1104113494178094 2212 14 1.8474280232458113 2212 15 1.773256370677828 2212 16 1.392165046142118 2212 17 1.6383098574172692 2212 18 1.791399783267714 2212 19 1.3868544613870242 2212 20 2.11059910309892 2212 21 2.2220702902924856 2212 22 2.507578863169634 2212 23 2.463396704666522 2212 24 1.81587517071128 2212 25 2.033336457030849 2213 1 0.5076184986558019 2213 2 0.5305438974192818 2213 3 0.23153145274035936 2213 4 0.574522350801459 2213 5 0.5253683456891522 2213 6 0.1893005801519223 2213 7 0.5923001257652096 2213 8 0.8393078241609757 2213 9 1.488490827259163 2213 10 -9.229892678959455 2213 11 -2.7499139891244084 2213 12 -0.8299359430416615 2213 13 0.06191400717983697 2213 14 0.5346832592641633 2213 15 0.36932197423968205 2213 16 0.7712402812518349 2213 17 0.573291230367154 2213 18 0.3688440156969506 2213 19 0.8888500544995637 2213 20 1.6153171985966281 2213 21 1.653241068539728 2213 22 1.7905055816285866 2213 23 1.2502748564996935 2213 24 1.4627767199366701 2213 25 1.0316327634231044 2214 1 -1.9645165489940482 2214 2 -2.307678673196687 2214 3 -2.1393425252614975 2214 4 -2.2150677718702987 2214 5 -1.9420387186032002 2214 6 -2.0320416737668268 2214 7 -9.760708013675124 2214 8 -3.480622247366334 2214 9 -2.791634649307852 2214 10 -2.08131157645769 2214 11 -2.8465987363303284 2214 12 -11.658946940285269 2214 13 -5.615419629475326 2214 14 -12.807429048384574 2214 15 -6.961328970854495 2214 16 -4.822846587562388 2214 17 -3.8220316749078087 2214 18 -3.365094316782148 2214 19 -3.5588867717856516 2214 20 -3.7921015783037717 2214 21 -12.435483307846123 2214 22 -14.789662286905152 2214 23 -9.317170853917492 2214 24 -7.833759198575738 2214 25 -15.271466600971479 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46461.0 3 1.0 4 794.0 5 7418.0 6 8777.0 7 7152.0 8 5733.0 9 4717.0 10 4246.0 11 3812.0 12 3617.0 13 3574.0 14 3528.0 15 3697.0 16 3732.0 17 4207.0 18 4525.0 19 5348.0 20 6752.0 21 8880.0 22 11227.0 23 13604.0 24 16062.0 25 18377.0 26 21413.0 27 23812.0 28 26918.0 29 31599.0 30 37622.0 31 45950.0 32 55999.0 33 68035.0 34 82247.0 35 98459.0 36 120101.0 37 159087.0 38 316001.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.30656744870435 18.805846149289884 12.901322655036509 24.986263746969257 2 17.18543626906668 21.643231449554868 36.83501840921045 24.336313872168 3 19.417765446613824 26.045895927365603 29.067160952075977 25.4691776739446 4 13.409360771151025 17.521994820348365 36.25475658442178 32.81388782407883 5 14.103024603390416 38.206197246308946 33.141219580798804 14.54955856950183 6 31.107894117628724 36.05996905245345 19.12943273739308 13.702704092524751 7 29.26102962659966 31.92146321509963 20.91470422096262 17.902802937338087 8 27.457865715920377 33.633611396483964 20.073161111899243 18.83536177569642 9 27.61465459475464 15.967259353913763 19.193794704773186 37.22429134655842 10 15.54307699739607 29.93285264826101 31.432019734371373 23.092050619971545 11 34.89662061292102 23.043365112807262 22.653471795846748 19.406542478424967 12 24.432195815986955 25.932185297126637 28.528317940734404 21.107300946152005 13 29.129037410447502 21.409465782082098 25.654303000081807 23.807193807388593 14 23.273290236523415 20.81946789438295 26.929636679400403 28.97760518969323 15 24.984767998304612 28.379595506598456 22.975546209452098 23.660090285644834 16 24.45511337163551 27.815280043756108 24.735289580343412 22.994317004264975 17 23.419244802887214 27.85462342602339 25.635687477453402 23.090444293635993 18 22.898434870414004 27.174570228255856 27.149170375356846 22.777824525973298 19 24.276739654324757 26.59142208672034 26.723954563410285 22.407883695544616 20 24.690913003289754 26.0254274757784 26.629033562728775 22.654625958203066 21 25.417092005704088 25.218773620927458 25.932330182577594 23.43180419079086 22 25.069034944460373 25.912726116714246 25.70024497276003 23.31799396606535 23 24.31126788108857 25.774090722443116 25.861903726715337 24.05273766975298 24 24.106262841275022 26.09713282621416 26.142011548479537 23.654592784031276 25 24.115355038368314 25.956831460691664 26.348189026561357 23.57962447437866 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 77.0 1 77.0 2 128.5 3 180.0 4 180.0 5 180.0 6 430.0 7 680.0 8 680.0 9 680.0 10 827.5 11 975.0 12 975.0 13 975.0 14 1271.5 15 1568.0 16 1568.0 17 1568.0 18 3766.0 19 5964.0 20 5964.0 21 5964.0 22 12048.5 23 18133.0 24 18133.0 25 18133.0 26 30428.0 27 42723.0 28 42723.0 29 42723.0 30 57162.0 31 71601.0 32 71601.0 33 71601.0 34 89533.0 35 107465.0 36 107465.0 37 107465.0 38 122138.0 39 136811.0 40 136811.0 41 136811.0 42 149031.0 43 161251.0 44 161251.0 45 161251.0 46 171546.5 47 181842.0 48 181842.0 49 181842.0 50 178960.5 51 176079.0 52 176079.0 53 176079.0 54 158571.5 55 141064.0 56 141064.0 57 141064.0 58 122057.0 59 103050.0 60 103050.0 61 103050.0 62 84503.0 63 65956.0 64 65956.0 65 65956.0 66 50833.0 67 35710.0 68 35710.0 69 35710.0 70 26790.0 71 17870.0 72 17870.0 73 17870.0 74 12808.5 75 7747.0 76 7747.0 77 7747.0 78 5867.0 79 3987.0 80 3987.0 81 3987.0 82 2806.5 83 1626.0 84 1626.0 85 1626.0 86 1155.0 87 684.0 88 684.0 89 684.0 90 552.5 91 421.0 92 421.0 93 421.0 94 220.0 95 19.0 96 19.0 97 19.0 98 10.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029840652474047203 2 0.012388155987920379 3 0.001168693961124564 4 0.0 5 0.003350256021890417 6 0.0017140844763160275 7 7.012163766747384E-4 8 0.043787067076800336 9 0.004986427567464807 10 0.002960691368182229 11 0.004363124121531706 12 0.00584346980562282 13 0.0014803456840911146 14 0.0013245198226078394 15 1.558258614832752E-4 16 4.6747758444982566E-4 17 0.0024153008529907657 18 9.349551688996513E-4 19 0.0019478232685409401 20 0.7898033789279804 21 0.015115108563877695 22 0.005921382736364458 23 0.00584346980562282 24 0.0019478232685409401 25 2.3373879222491283E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1283484.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.14453047360029 #Duplication Level Percentage of deduplicated Percentage of total 1 79.68174810906848 47.92421327335611 2 11.865758201422825 14.273209114756954 3 3.681857944627269 6.643308520503062 4 1.6302296394177105 3.921975849076996 5 0.8371378902944226 2.5174632676709177 6 0.5128403651317331 1.8506725781254691 7 0.3241756525082319 1.364817468775643 8 0.2223012915876644 1.0696165444971988 9 0.17062213562677767 0.9235789412107943 >10 0.9514294724550607 10.703616990147736 >50 0.08002238821436912 3.3254274024779624 >100 0.03960969786772877 4.28076503240477 >500 0.0013336539868854655 0.504373011075204 >1k 9.335577908198259E-4 0.696962005921191 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTATCAACGCAGAGTACATGGG 1526 0.11889513231173898 No Hit GTATCAACGCAGAGTACTTTTTTTT 1513 0.1178822642120977 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 7.79129307416376E-5 10 0.0 0.0 0.0 0.0 7.79129307416376E-5 11 0.0 0.0 0.0 0.0 7.79129307416376E-5 12 0.0 0.0 0.0 0.0 7.79129307416376E-5 13 0.0 0.0 0.0 0.0 7.79129307416376E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACCCG 40 2.8074402E-4 16.588404 5 CCCGTCT 40 0.005356169 14.218632 8 CGTGCGC 40 0.0053741997 14.211429 10 AGGCGTG 75 2.1161429E-4 11.374905 7 TTGACCG 95 1.2818531E-5 11.05855 18 ATACGAC 60 0.0059944154 11.053334 13 CCACGTC 60 0.0059944154 11.053334 9 GGTGGTA 260 0.0 10.933998 1 AAAGGCG 80 3.8472476E-4 10.663974 5 ACCGAGT 90 9.726044E-5 10.532319 8 TAGACTA 100 2.4564886E-5 10.426995 5 GGATATG 305 0.0 10.252864 1 AGGACCG 130 3.955447E-7 10.208248 5 TTTTATA 140 9.982796E-8 10.156957 2 TAGATTG 75 0.0026958613 10.111027 5 TGACCGA 95 1.5581866E-4 10.052832 19 AAGGCGT 95 1.6802612E-4 9.977986 6 TCTATAC 115 1.0533917E-5 9.890837 3 TTAGACT 125 2.6410307E-6 9.858251 4 TGTACTG 175 2.6684575E-9 9.749918 5 >>END_MODULE