FastQCFastQC Report
Thu 26 May 2016
SRR1364252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501287
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTT42550.2834234893128363No Hit
GTATCAACGCAGAGTACTTTTTTTT42510.2831570512500275No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32130.2140163739511499No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN30550.20349207047020323No Hit
GGTATCAACGCAGAGTACTTTTTTT29180.19436656681900263No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA20200.13455122171843226No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15570.10371101594831633No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA250.006027075519.0026742
ATCACGC651.9093568E-716.0797233
CTCAACG1550.015.3252494
TATAACG755.8347723E-815.2021392
TCTCGTC456.772048E-414.77491912
TAACGCA1058.913048E-1114.4787114
CGCATAT759.696778E-713.9301748
ACATGGT10050.013.8033983
GGTATCA22800.013.7102621
CATGGTA8150.013.6404124
TCAACGG1351.8189894E-1213.3726993
ATAACGC1001.00462785E-813.3023173
TCACGCA905.3931035E-712.6688724
GTATAAC2250.012.2461681
CAACGGA1650.012.0930154
TTTGCGC2850.012.002892517
AGTATCG951.0421063E-611.997685
TTGCGCG2950.011.91693118
GTCCTAA1209.98989E-911.8766711
TACATGT12000.011.5599592
Produced by FastQC (version 0.11.5)