FastQCFastQC Report
Thu 26 May 2016
SRR1364252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501287
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTT171371.1414872705885017No Hit
GTATCAACGCAGAGTACTTTTTTTT152711.0171939142882074No Hit
GGTATCAACGCAGAGTACTTTTTTT123000.8192970431369885No Hit
GTACTTTTTTTTTTTTTTTTTTTTT79890.5321434209448294No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59640.39725915164788617No Hit
GAGTACTTTTTTTTTTTTTTTTTTT50140.33398011173080167No Hit
GCAGAGTACTTTTTTTTTTTTTTTT25780.17171933148025662No Hit
GGTATCAACGCAGAGTACATGGGAT25690.17111984583893686No Hit
ATCAACGCAGAGTACTTTTTTTTTT19660.13095430787051376No Hit
GGTATCAACGCAGAGTACATGGGAG19660.13095430787051376No Hit
GTGGTATCAACGCAGAGTACATGGG19390.12915585094655452No Hit
GGTATCAACGCAGAGTACATGGGAA16730.11143771976977086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCGA509.0342524E-515.13572910
CAACGCC2250.014.7211774
GGTATCA35500.014.3929581
CGCGCCA400.00542947214.18974610
GCCGAGT2250.013.8804298
TATCACG751.0095882E-613.8804282
AACGCCG2250.013.8799675
GTTAGAC552.0249235E-413.7652563
CGAGTAC2350.013.686563510
ACGCCGA2300.013.578686
TATAACG1051.4497346E-913.5198992
TGCACCG500.001543493813.2490585
CCGAGTA2300.012.7502069
TGACCGA701.0053824E-412.32146519
GTGCGCC550.003168067412.03938311
ACCAGTT1501.4551915E-1111.98205412
ATCACGC951.0889908E-611.9540393
CGCCGAG2550.011.8763037
TCCGGTG1604.9112714E-1111.23317512
TCACGCA1201.3442877E-711.0408834
Produced by FastQC (version 0.11.5)