FastQCFastQC Report
Thu 26 May 2016
SRR1364251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5255532
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN113700.21634346437239843No Hit
GTATCAACGCAGAGTACTTTTTTTT86220.16405570359004568No Hit
TATCAACGCAGAGTACTTTTTTTTT59490.11319501051463488No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA52630.10014209788847257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43050.012.7570131
TAACGCA2050.011.5888024
TGACCGA6400.011.4325319
GTATCAA108250.011.2701961
TTGACCG7100.010.97424618
ACATGGT22650.010.9502663
CTCAACG3950.010.82599454
TACATGT25750.010.1476712
ACGGCCC3200.010.09587515
GTACTAG3400.010.0604611
AACGCAT12300.09.7296696
ACGCATA11500.09.66317
TCAACTC25550.09.6329934
GTATTAG7350.09.5661831
GGCGTAG1901.0586518E-99.49746610
TGTGCGA1804.2109605E-99.49746510
GCTTATA11350.09.459691
TATCACG1356.837894E-69.1499872
ATGCACT10000.09.12270454
CCCGTAC950.00182004449.0026683
Produced by FastQC (version 0.11.5)