FastQCFastQC Report
Thu 26 May 2016
SRR1364251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5255532
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT295300.5618841251466073No Hit
TATCAACGCAGAGTACTTTTTTTTT227020.431963881106613No Hit
GGTATCAACGCAGAGTACTTTTTTT186620.35509250062600706No Hit
GTACTTTTTTTTTTTTTTTTTTTTT104130.19813408043181926No Hit
ACGCAGAGTACTTTTTTTTTTTTTT97840.18616573926293284No Hit
GAGTACTTTTTTTTTTTTTTTTTTT72780.13848265028164608No Hit
GTGGTATCAACGCAGAGTACATGGG59190.11262418343185808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69950.013.2885611
ACGGCCC4500.012.52797315
TATCACG2050.012.0207612
TATAACG3200.011.8473842
TAACGCA3050.011.4961474
CGCGCCA2350.011.28573410
GCCCCCG5150.010.94666918
GGTTGCG803.8600233E-410.66142757
TCGCGGT1907.2759576E-1210.561048517
CGGCCCC5250.010.55625816
TGCACGG5900.010.27446712
GTATCAA147700.010.2003091
CCGAGTA2800.010.1488129
GTGGTAT21300.010.1426611
ACGCCGA2850.09.9756046
TGGTATC21550.09.8517462
TGACCGA4850.09.65346419
CCTATAA9550.09.6242081
AACTGGC8600.09.55523517
ATAACGC3700.09.4764643
Produced by FastQC (version 0.11.5)